Alterations in the pattern of polypeptide synthesis resulting from amino acid limitation in Neurospora crassa

Two-dimensional electrophoresis was used to examine the pattern of polypeptide synthesis in Neurospora crassa mycelium of an arg-6 strain grown in the presence of [14C]arginine. Reduction in the arginine supply rate led to amino acid limited growth, and to major alterations in the pattern of polypeptide synthesis. Strains carrying wild-type (cpc-1+) or mutant (cpc-1) alleles at a locus governing cross-pathway control of amino acid synthetic enzymes differed markedly with respect to their pattern of polypeptide synthesis under limitation conditions, but differed little during arginine sufficient growth. Among 160 abundant polypeptide species classified for their response to limitation in two dimensional fluorographs, 31 were induced by limitation only in a cpc-1+ strain, 13 only in a cpc-1 strain, and 9 showed induction in both strains.