Survival of and plasmid stability in Pseudomonas and Klebsiella spp. introduced into agricultural drainage water

1989 ◽  
Vol 35 (7) ◽  
pp. 675-680 ◽  
Author(s):  
J. T. Trevors ◽  
J. D. Van Elsas ◽  
M. E. Starodub ◽  
L. S. Van Overbeek
Keyword(s):  
Pseudomonas Fluorescens ◽  
Plasmid Stability ◽  
Drainage Water ◽  
Good Survival ◽  
Agricultural Drainage ◽  
Klebsiella Aerogenes ◽  
Plasmid Pbr322 ◽  
Bacterial Strains ◽  
Agricultural Drainage Water ◽  
Aerobic And Anaerobic

Cell survival and plasmid stability in Pseudomonas fluorescens R2f and Pseudomonas putida CYM 318 containing respectively, plasmid RP4 and pRK2501, and Klebsiella aerogenes NCTC 418 harboring plasmid pBR322 were studied in sterile and nonsterile agricultural drainage water under both aerobic and anaerobic conditions and in the absence and presence of added nutrients. Both Pseudomonas strains survived well in sterile drainage water incubated aerobically, with or without added nutrients. However, Klebsiella aerogenes NCTC 418 (pBR322) only survived in the presence of added nutrients. Pseudomonas fluorescens R2f (RP4) and K. aerogenes NCTC 418 (pBR322) did not survive under anerobic conditions without added nutrients, but showed good survival in the presence of nutrients. Survival of all three strains was negatively affected in nonsterile agricultural drainage water when compared with survival in sterile water. Maintenance of the three plasmids was host, plasmid, and environment dependent. Plasmid pBR322 was not stably maintained in K. aerogenes NCTC 418 under all conditions used in the study, and pRK2501 was readily lost from P. putida CYM 318. Maintenance of RP4 by P. fluorescens R2f was markedly influenced by added nutrients, which caused a loss of the plasmid from cells. The results of the present study demonstrate the influence of nutrients, O2, and native microorganisms on the survival of introduced bacterial strains and plasmid stability in agricultural drainage water.Key words: bacteria, survival, stability, plasmids, water.

scholarly journals Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water

2021 ◽  
Vol 9 (6) ◽  
pp. 1331
Author(s):  
Arnaud Jéglot ◽  
Sebastian Reinhold Sørensen ◽  
Kirk M. Schnorr ◽  
Finn Plauborg ◽  
Lars Elsgaard
Keyword(s):  
Temperature Response ◽  
Microbial Transformation ◽  
Drainage Water ◽  
Microbial Populations ◽  
Agricultural Drainage ◽  
Bacterial Populations ◽  
Cold Temperatures ◽  
Cold Adapted ◽  
Agricultural Drainage Water ◽  
Temperature Responses

Denitrifying woodchip bioreactors (WBR), which aim to reduce nitrate (NO3−) pollution from agricultural drainage water, are less efficient when cold temperatures slow down the microbial transformation processes. Conducting bioaugmentation could potentially increase the NO3− removal efficiency during these specific periods. First, it is necessary to investigate denitrifying microbial populations in these facilities and understand their temperature responses. We hypothesized that seasonal changes and subsequent adaptations of microbial populations would allow for enrichment of cold-adapted denitrifying bacterial populations with potential use for bioaugmentation. Woodchip material was sampled from an operating WBR during spring, fall, and winter and used for enrichments of denitrifiers that were characterized by studies of metagenomics and temperature dependence of NO3− depletion. The successful enrichment of psychrotolerant denitrifiers was supported by the differences in temperature response, with the apparent domination of the phylum Proteobacteria and the genus Pseudomonas. The enrichments were found to have different microbiomes’ composition and they mainly differed with native woodchip microbiomes by a lower abundance of the genus Flavobacterium. Overall, the performance and composition of the enriched denitrifying population from the WBR microbiome indicated a potential for efficient NO3− removal at cold temperatures that could be stimulated by the addition of selected cold-adapted denitrifying bacteria.

Hydrology of Wetland Buffer Areas for Pumped Agricultural Drainage Water

1988 ◽  
pp. 260-274 ◽  
Author(s):  
G. M. Chescheir ◽  
R. W. Skaggs ◽  
J. W. Gilliam ◽  
R. G. Broadhead
Keyword(s):  
Drainage Water ◽  
Agricultural Drainage ◽  
Agricultural Drainage Water
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