Erratum: Evaluation of β-glucuronidase assay for the detection of Escherichia coli from environmental waters

1992 ◽  
Vol 38 (5) ◽  
pp. 443-443
Author(s):  
Lois C. Shadix ◽  
Eugene W. Rice
Keyword(s):  
Escherichia Coli ◽  
Environmental Waters ◽  
Glucuronidase Assay

An Improved Membrane Filtration Method for Enumeration of Faecal Coliforms and E. Coli by a Fluorogenic β-Glucuronidase Assay

1993 ◽  
Vol 27 (3-4) ◽  
pp. 267-270 ◽  
Author(s):  
M. T. Augoustinos ◽  
N. A. Grabow ◽  
B. Genthe ◽  
R. Kfir
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Membrane Filtration ◽  
Faecal Coliforms ◽  
Environmental Waters ◽  
Filtration Method ◽  
E Coli ◽  
Wide Range ◽  
Pure Cultures ◽  
Glucuronidase Assay

A fluorogenic β-glucuronidase assay comprising membrane filtration followed by selective enumeration on m-FC agar at 44.5°C and further confirmation using tlie 4-metliylumbelliferyl-β-D-glucuronide (MUG) containing medium was evaluated for the detection of Escherichia coli in water. A total of 200 typical blue and non-typical blue colonies were isolated from sea and fresh water samples using initial selective enumeration on m-FC agar. Pure cultures of the selected colonies were further tested using the MUG assay and identified using the API 20E method. Of the colonies tested which were shown to be positive using the MUG assay 99.4% were Escherichia coli. The results of this study indicate the combination of the m-FC method followed by the MUG assay to be highly efficient for the selection and confirmation of E. coli from a wide range of environmental waters.

Evaluation of β-glucuronidase assay for the detection of Escherichia coli from environmental waters

1991 ◽  
Vol 37 (12) ◽  
pp. 908-911 ◽  
Author(s):  
Lois C. Shadix ◽  
Eugene W. Rice
Keyword(s):  
Escherichia Coli ◽  
Accurate Method ◽  
Fecal Coliforms ◽  
Fluorogenic Substrate ◽  
Environmental Waters ◽  
E Coli ◽  
Glucuronidase Activity ◽  
Gas Fermentation ◽  
Fermentation Method ◽  
Glucuronidase Assay

The new United States Drinking Water Regulations state that water systems must analyze for Escherichia coli or fecal coliforms on any routine or repeat sample that is positive for total coliforms. The proposed methods for the detection of E. coli are based on β-glucuronidase activity, using the fluorogenic substrate 4-methylumbelliferyl β-D-glucuronide (MUG). This study was conducted to determine whether β-glucuronidase negative E. coli were present in significant numbers in environmental waters. Two hundred and forty E. coli cultures were isolated from 12 water samples collected from different environmental sources. β-glucuronidase activity was determined using lauryl tryptose broth with MUG, EC broth with MUG, and the Autoanalysis Colilert (AC) procedure. The isolates were also evaluated by the standard EC broth gas fermentation method for fecal coliforms. The results confirm that assaying for the enzyme β-glucuronidase utilizing the MUG substrate is an accurate method for the detection of E. coli in environmental waters. Key words: Escherichia coli, β-glucuronidase, 4-methylumbelliferyl β-D-glucuronide, water.

Comparison of the β-Glucuronidase Assay and the Conventional Method for Identification of Escherichia coli on Eosin-Methylene Blue Agar

1997 ◽  
Vol 60 (1) ◽  
pp. 6-9 ◽  
Author(s):  
SHIU W. HUANG ◽  
CHUNG H. CHANG ◽  
TUNG F. TAI ◽  
TSUNG C. CHANG
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Conventional Method ◽  
Meat Products ◽  
Mixed Cultures ◽  
Effective Alternative ◽  
Methyl Red ◽  
Raw Meat ◽  
E Coli ◽  
Glucuronidase Assay

The purpose of this study was to compare the IMViC (indole, methyl red, Voges-Proskauer and citrate utilization) tests with the β-glucuronidase (GUD) assay for the identification of suspect Escherichia coli on Levine's eosin-methylene blue (EMB) agar. After testing 258 suspect E. coli colonies from raw meat and meat products, 163 and 44 were found to be E. coli and non-E. coli, respectively, by both methods. Nine isolates were IMViC positive (i.e., + + − − or − + − −) but GUD negative; among these isolates, six were confirmed to be E. coli by API 20E (bioMérieux, Marcy-I'Etoile, France) with the remaining three being non-E. coli. There were 42 isolates that were IMViC negative but GUD positive; among these isolates, seven were pure E. coli cultures, 33 were mixed cultures containing E. coli, and the remaining two were Proteus spp. The sensitivities for the identification of E. coli on EMB were 80.9% (169/209) and 97.1% (203/209), respectively, by the IMViC tests and GUD assay; whereas the specificities were 93.9% (46/49) and 95.9% (47/49), respectively, by the IMViC tests and GUD assay. It is proposed that the GUD assay can be an effective alternative to the conventional IMViC tests for the identification of suspect E. coli on EMB.

scholarly journals Corrigendum to “A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters” [Water Res. 126, 101–110]

2019 ◽  
Vol 161 ◽  
pp. 652
Author(s):  
David I. Walker ◽  
Jonathan McQuillan ◽  
Michael Taiwo ◽  
Rachel Parks ◽  
Craig A. Stenton ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Environmental Waters

Glucuronidase Assay in a Rapid MPN Determination for Recovery of Escherichia coli from Selected Foods

1987 ◽  
Vol 70 (1) ◽  
pp. 31-34
Author(s):  
Wallace H Andrews ◽  
Clyde R Wilson ◽  
Paul L Poelma
Keyword(s):  
Escherichia Coli ◽  
False Positive ◽  
Uv Light ◽  
Most Probable Number ◽  
Probable Number ◽  
Rapid Methods ◽  
Glucuronidase Assay

Abstract Glucuronidase is present in most strains of Escherichia coli but absent in most other enteric microorganisms; therefore, an assay for this enzyme is useful for determining the presence of the organism. The substrate 4-methylumbelliferyl beta-D-glucuronide (MUG) is incorporated into either lauryl tryptose (LT) broth or EC medium; the inoculated tubes are then incubated under specified conditions and examined under longwave UV light for the presence of a fluorogenic glucuronidase end product. When compared with the 10-day most probable number (MPN) procedure of AOAC, the LT-MUG and the EC-MUG tests required 24 and 96 h, respectively, and gave comparable mean log MPN values for samples of crabmeat, sunflower kernels, and walnut pieces. However, false-positive and falsenegative reactions were observed with foods tested by both of these rapid methods. Overall, method sensitivity was not compromised by using the LT-MUG rather than the EC-MUG method. Incorporation of 25 αg MUG/mL into LT broth resulted in diminished fluorescence of positive reactions, whereas MUG concentrations of 50 and 100 αg/mL provided decisive fluorogenic reactions.

scholarly journals Rapid and Quantitative Detection of Escherichia coli and Enterococcus in Environmental Waters Using Real-Time PCR

2013 ◽  
Vol 36 (6) ◽  
pp. 191-197
Author(s):  
Yudai OGURA ◽  
Junichi YAGUCHI ◽  
Toshiya KOMATSU
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Detection ◽  
Environmental Waters

scholarly journals Conjugative multiple-antibiotic resistance plasmids in Escherichia coli isolated from environmental waters contaminated by human faecal wastes

2014 ◽  
Vol 118 (2) ◽  
pp. 399-411 ◽  
Author(s):  
E. Laroche-Ajzenberg ◽  
A. Flores Ribeiro ◽  
J. Bodilis ◽  
W. Riah ◽  
S. Buquet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Multiple Antibiotic Resistance ◽  
Environmental Waters ◽  
Resistance Plasmids

scholarly journals Pathogenic Escherichia coli Found in Sewage Treatment Plants and Environmental Waters

2012 ◽  
Vol 78 (16) ◽  
pp. 5536-5541 ◽  
Author(s):  
E. M. Anastasi ◽  
B. Matthews ◽  
H. M. Stratton ◽  
M. Katouli
Keyword(s):  
Escherichia Coli ◽  
Animal Species ◽  
Sewage Treatment ◽  
Environmental Waters ◽  
Phylogenetic Groups ◽  
Content Type ◽  
Sewage Treatment Plants ◽  
E Coli ◽  
Nonpoint Sources ◽  
Pathogenic Escherichia Coli

ABSTRACTWe previously demonstrated that someEscherichia colistrains with uropathogenic properties survived treatment stages of sewage treatment plants (STPs), suggesting that they may be released into the environment. We investigated the presence of such strains in the surrounding environmental waters of four STPs from which these persistent strains were isolated. In all, 264E. coliisolates were collected from 129 receiving water sites in a 20-km radius surrounding STPs. We also included 93E. colistrains collected from 18 animal species for comparison. Isolates were typed using a high-resolution biochemical fingerprinting method (the PhPlate system), and grouped into common (C) types. One hundred forty-seven (56%) environmental isolates were identical to strains found in STPs' final effluents. Of these, 140 (95%) carried virulence genes (VGs) associated with intestinal pathogenicE. coli(IPEC) or uropathogenicE. coli(UPEC) and were found in a variety of sites within areas sampled. Of the remaining 117 environmental strains not identical to STP strains, 105 belonged to 18 C types and 102 of them carried VGs found among IPEC or UPEC strains. These strains belonged mainly to phylogenetic groups A (A0 and A1) and B1 and to a lesser extent B22, B23, D1, and D2. Eight of 18 environmental C types, comprising 50 isolates, were also identical to bird strains. The presence of a high percentage of environmentalE. coliin waters near STPs carrying VGs associated with IPEC and UPEC suggests that they may have derived from STP effluents and other nonpoint sources.

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