SEPARATION OF THE VALYL-LEUCYL- AND VALYL-GLUTAMYL-POLYPEPTIDE CHAINS OF HORSE GLOBIN BY FRACTIONAL PRECIPITATION AND COLUMN CHROMATOGRAPHY

1959 ◽  
Vol 37 (3) ◽  
pp. 405-416 ◽  
Author(s):  
Strathearn Wilson ◽  
David B. Smith
Keyword(s):  
Ion Exchange ◽  
Column Chromatography ◽  
Exchange Resin ◽  
Gradient Elution ◽  
Ion Exchange Resin ◽  
The Other ◽  
Terminal Sequence ◽  
Fractional Precipitation ◽  
Molecular Weights ◽  
Polypeptide Chains

Horse globin has been fractionated into two components by stepwise addition of acid acetone to an acidified globin solution and by gradient elution with urea from a carboxylic-type ion exchange resin at low pH.The components obtained by both methods are similar to those prepared earlier by electrophoresis. They have similar molecular weights (about 16,000) and appear to be present in nearly equal amounts. One has the N-terminal sequence valyl-leucyl- and the other valyl-glutamyl-. They differ electrokinetically and in other properties.

SEPARATION OF THE VALYL-LEUCYL- AND VALYL-GLUTAMYL-POLYPEPTIDE CHAINS OF HORSE GLOBIN BY FRACTIONAL PRECIPITATION AND COLUMN CHROMATOGRAPHY

1959 ◽  
Vol 37 (1) ◽  
pp. 405-416 ◽  
Author(s):  
Strathearn Wilson ◽  
David B. Smith
Keyword(s):  
Ion Exchange ◽  
Column Chromatography ◽  
Exchange Resin ◽  
Gradient Elution ◽  
Ion Exchange Resin ◽  
The Other ◽  
Terminal Sequence ◽  
Fractional Precipitation ◽  
Molecular Weights ◽  
Polypeptide Chains

Horse globin has been fractionated into two components by stepwise addition of acid acetone to an acidified globin solution and by gradient elution with urea from a carboxylic-type ion exchange resin at low pH.The components obtained by both methods are similar to those prepared earlier by electrophoresis. They have similar molecular weights (about 16,000) and appear to be present in nearly equal amounts. One has the N-terminal sequence valyl-leucyl- and the other valyl-glutamyl-. They differ electrokinetically and in other properties.

A pilot plant for the removal of cationic fission products from milk: I. Design and construction

1968 ◽  
Vol 35 (2) ◽  
pp. 257-268 ◽  
Author(s):  
R. F. Glascock ◽  
H. S. Hall ◽  
S. F. Suffolk ◽  
D. T. W. Bryant
Keyword(s):  
Citric Acid ◽  
Ion Exchange ◽  
Pilot Plant ◽  
Potassium Hydroxide ◽  
Exchange Resin ◽  
Ionic Composition ◽  
Fission Products ◽  
Ion Exchange Resin ◽  
The Other ◽  
Design And Construction

SummaryA pilot plant with a capacity of 2300 1./5 h day for the removal of cationic fission products from milk is described. The process involves the acidification of the milk with citric acid to pH 5.25 and its passage through an ion exchange resin charged with the ions of Ca, K, Na and Mg in the same proportions as those in which they occur in milk. The effluent milk is neutralized with potassium hydroxide. At the end of the day the plant and resin bed are washed and sterilized.Two resin beds are provided and are used on alternate days, one being washed and regenerated while the other is in use. Regeneration is carried out with a solution which removes radioactive cations and restores the resin bed to its original ionic composition.Bacteriological tests show that the method of cleaning both plant and resin bed is satisfactory.Conclusions are drawn as to a suitable design for a larger scale plant.

Effect of adsorbed metal ions and buffer nature on IgG separation from human plasma by column chromatography using an ion exchange resin, Amberlite IRC-718

2010 ◽  
Vol 115 (1) ◽  
pp. 324-329 ◽  
Author(s):  
Noureddine Charef ◽  
Lekhmici Arrar ◽  
Afaf Lamaaoui ◽  
Hiba Boudjellal ◽  
Abderrahmane Baghiani ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Metal Ions ◽  
Human Plasma ◽  
Column Chromatography ◽  
Exchange Resin ◽  
Ion Exchange Resin

Evaluation of Ion Exchange Resins and Various Enzymes in Thiamine Analysis

1981 ◽  
Vol 64 (6) ◽  
pp. 1336-1338
Author(s):  
Wayne C Ellefson ◽  
Earl Richter ◽  
Mark Adams ◽  
N Thomas Baillies
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
The Other ◽  
Ion Exchange Resins ◽  
Enzyme Preparations ◽  
Hydrogen Form ◽  
Aoac Method ◽  
Exchange Resins

Abstract Four ion exchange resins and 9 enzyme preparations are evaluated for use in the official AOAC thiamine method because Decalso and Clarase or Mylase P either are no longer available or are available in a form that is not suitable for use in the assay. The enzymes are prepared in the same manner described for Clarase or Mylase P in the AOAC method and are compared with Clarase T300 for their effectiveness in releasing thiamine from thiamine phosphate, and their ability to produce similar results on samples. Rhozyme S is 90–100– as effective as Clarase T300 in both of these respects. The other enzymes tested were not satisfactory. Further study is necessary because Rhozyme S also is no longer manufactured. The ion exchange resins are prepared for use in the manner described for Decalso in the AOAC method. Recoveries of thiamine range from 95 to 100%, using Bio-Rex 70 (hydrogen form) ion exchange resin. The other resins tested were not satisfactory.

scholarly journals Comparative study of various methods in the estimation of blood HbA1c and its outcome

2020 ◽  
Vol 11 (SPL2) ◽  
pp. 43-47
Author(s):  
Sabarinathan M ◽  
Ananthi N ◽  
Ashwin Raj H
Keyword(s):  
Diabetes Mellitus ◽  
Ion Exchange ◽  
Column Chromatography ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Test Method ◽  
Clinical Laboratories ◽  
Turbidimetric Assay ◽  
Hba1c Levels

Diabetes mellitus is a major health problem all over the world. Blood glucose measurement has limited value in assessing the long term glycaemic control. Estimation of HbA1c is now routinely used in clinical laboratories for long term assessment of glyceamic control. The result of different methods of HbA1C estimation has a lot of variations and hence it is essential to compare their results. This study is aimed to assess the accuracy and reliability of estimation of HbA1c levels by PEITT and Column Chromatography with ion-exchange resin by comparing it with the hba1c levels of High-performance liquid chromatography (HPLC) which is a reference method. We have included 50 patients of type 2 diabetes mellitus in our study. HbA1c was determined using BIO RAD D-10 HbA1c Analyzer, Immuno-turbidimetric assay in ERBA-Chem semi automated analyzer and Column chromatography with Ion-exchange resin method in ERBA-Chem semi automated analyzer. The results obtained from Immuno-turbidimetric assay and Ion-exchange resin methods were compared with the results of BIO RAD D-10 HbA1c Analyzer. The results obtained from Particle Enhanced Immuno-Turbidimetric Test method showed a better correlation with BIO RAD D-10 HbA1c Analyzer than Column chromatography with Ion-exchange resin (CCG-IER). Hence, Particle Enhanced Immuno-Turbidimetric Test method is more reliable and accurate and can be used as an alternative method to HPLC in clinical laboratories.

Characterization of Fruit Juices by Acid Profiles

1963 ◽  
Vol 46 (3) ◽  
pp. 365-371 ◽  
Author(s):  
David Jorysch
Keyword(s):  
Ion Exchange ◽  
Analytical Methods ◽  
Exchange Resin ◽  
Gradient Elution ◽  
Ion Exchange Resin ◽  
Fruit Juices ◽  
Acid Recovery ◽  
Valuable Adjunct ◽  
Elution Chromatography

Abstract The gradient elution technique has been applied to the investigation of organic acids present in fruit juices. In this technique, fruit juices are placed on columns containing Dowex ion exchange Resin, 1-X10, soluble fruit solids other than acids are eluted with water, and the acids are eluted with formic acid. Recovery data are given and evaluated on a number of fruit juices. Gradient elution chromatography appears to be a valuable adjunct to present analytical methods for fruit juices. Further work is necessary to determine the constancy of values, especially with regard to the varying degrees of ripeness of the fruit at the time of picking.

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