scholarly journals The fractionation of phosphate esters on ion-exchange resin by a new system of pH-gradient elution

1960 ◽  
Vol 77 (3) ◽  
pp. 534-542 ◽  
Author(s):  
H. E. Wade
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Gradient Elution ◽  
Ion Exchange Resin ◽  
Ph Gradient ◽  
Phosphate Esters ◽  
Ph Gradient Elution ◽  
New System

SEPARATION OF THE VALYL-LEUCYL- AND VALYL-GLUTAMYL-POLYPEPTIDE CHAINS OF HORSE GLOBIN BY FRACTIONAL PRECIPITATION AND COLUMN CHROMATOGRAPHY

1959 ◽  
Vol 37 (3) ◽  
pp. 405-416 ◽  
Author(s):  
Strathearn Wilson ◽  
David B. Smith
Keyword(s):  
Ion Exchange ◽  
Column Chromatography ◽  
Exchange Resin ◽  
Gradient Elution ◽  
Ion Exchange Resin ◽  
The Other ◽  
Terminal Sequence ◽  
Fractional Precipitation ◽  
Molecular Weights ◽  
Polypeptide Chains

Horse globin has been fractionated into two components by stepwise addition of acid acetone to an acidified globin solution and by gradient elution with urea from a carboxylic-type ion exchange resin at low pH.The components obtained by both methods are similar to those prepared earlier by electrophoresis. They have similar molecular weights (about 16,000) and appear to be present in nearly equal amounts. One has the N-terminal sequence valyl-leucyl- and the other valyl-glutamyl-. They differ electrokinetically and in other properties.

SEPARATION OF THE VALYL-LEUCYL- AND VALYL-GLUTAMYL-POLYPEPTIDE CHAINS OF HORSE GLOBIN BY FRACTIONAL PRECIPITATION AND COLUMN CHROMATOGRAPHY

1959 ◽  
Vol 37 (1) ◽  
pp. 405-416 ◽  
Author(s):  
Strathearn Wilson ◽  
David B. Smith
Keyword(s):  
Ion Exchange ◽  
Column Chromatography ◽  
Exchange Resin ◽  
Gradient Elution ◽  
Ion Exchange Resin ◽  
The Other ◽  
Terminal Sequence ◽  
Fractional Precipitation ◽  
Molecular Weights ◽  
Polypeptide Chains

Horse globin has been fractionated into two components by stepwise addition of acid acetone to an acidified globin solution and by gradient elution with urea from a carboxylic-type ion exchange resin at low pH.The components obtained by both methods are similar to those prepared earlier by electrophoresis. They have similar molecular weights (about 16,000) and appear to be present in nearly equal amounts. One has the N-terminal sequence valyl-leucyl- and the other valyl-glutamyl-. They differ electrokinetically and in other properties.

Modeling of salt and pH gradient elution in ion-exchange chromatography

2014 ◽  
Vol 37 (1-2) ◽  
pp. 5-13 ◽  
Author(s):  
Michael Schmidt ◽  
Mathias Hafner ◽  
Christian Frech
Keyword(s):  
Ion Exchange ◽  
Gradient Elution ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Ph Gradient ◽  
Ph Gradient Elution

Characterization of Fruit Juices by Acid Profiles

1963 ◽  
Vol 46 (3) ◽  
pp. 365-371 ◽  
Author(s):  
David Jorysch
Keyword(s):  
Ion Exchange ◽  
Analytical Methods ◽  
Exchange Resin ◽  
Gradient Elution ◽  
Ion Exchange Resin ◽  
Fruit Juices ◽  
Acid Recovery ◽  
Valuable Adjunct ◽  
Elution Chromatography

Abstract The gradient elution technique has been applied to the investigation of organic acids present in fruit juices. In this technique, fruit juices are placed on columns containing Dowex ion exchange Resin, 1-X10, soluble fruit solids other than acids are eluted with water, and the acids are eluted with formic acid. Recovery data are given and evaluated on a number of fruit juices. Gradient elution chromatography appears to be a valuable adjunct to present analytical methods for fruit juices. Further work is necessary to determine the constancy of values, especially with regard to the varying degrees of ripeness of the fruit at the time of picking.

OPTIMIZATION OF DNA EXTRACTION METHODS FROM BIOLOGICAL MATERIALS OF HONEY BEES IN A DIFFERENT METAMOTPHOSIS PHASE

2016 ◽  
Vol 52 ◽  
pp. 171-176
Author(s):  
M. Palkina ◽  
O. Metlitska
Keyword(s):  
Ion Exchange ◽  
Dna Extraction ◽  
Honey Bees ◽  
Biological Material ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Extraction Methods ◽  
Chelex 100 ◽  
Drone Brood ◽  
Dna Extraction Methods

The aim of the research – adaptation, optimization and using of existing DNA extraction methods from bees’ biological material with the reagent «Chelex-100" under complex economic conditions of native laboratories, which will optimize labour costs and improve the economic performance of DNA extraction protocol. Materials and methods. In order to conduct the research the samples of honey bees’ biological material: queen pupae exuviae, larvae of drone brood, some adult bees’ bodies (head and thorax) were selected. Bowl and drone brood were obtained from the experimental bee hives of Institute of Apiculture nd. a. P. I. Prokopovich of NAAS. DNA extraction from biosamples of Apis mellifera ssp. was carried out using «Chelex-100®» ion exchange resin in different concentrations and combinations. Before setting tests for determination of quantitative and quality indexes, dilution of DNA samples of the probed object was conducted in ratio 1:40. The degree of contamination with protein and polysaccharide fractions (OD 260/230), quantitative content of DNA (OD 260/280) in the extracted tests were conducted using spectrophotometer of «Biospec – nano» at the terms of sample volume in 2 µl and length of optical way in 0,7 mm [7]. Verification of DNA samples from biological material of bees, isolated by «Chelex-100®», was conducted after cold keeping during 24 hours at 20°C using PСR with primaries to the fragment of gene of quantitative trait locus (QTL) Sting-2 of next structure [8]:  3' – CTC GAC GAG ACG ACC AAC TTG – 5’; 3' – AAC CAG AGT ATC GCG AGT GTT AC – 5’ Program of amplification: 94 °C – 5 minutes – 1 cycle; 94 °C – 1 minute, 57°C – 1 minute, 72 °C – 2 minutes – 30 cycles; elongation after 72°C during 2 minutes – 1 cycle. The division of obtained amplicons was conducted by gel electrophoresis at a low current – 7 µÀ, in 1,5 % agarose gel (Sigma ®) in TAE buffer [7]. The results. At the time of optimization of DNA isolation methods, according to existing methods of foreign experts, it was found optimal volume of ion exchange resin solution was in the proposed concentration: instead of 60 µl of solution used 120 µl of «Chelex-100®», time of incubation was also amended from 30 minutes to 180 minutes [9]. The use of the author's combination of method «Chelex-100®» with lysis enzymes, proteinase K and detergents (1M dithiothreitol), as time of incubation was also amended, which was reduced to 180 minutes instead of the proposed 12 hours [10]. Changes in quality characteristics of obtained DNA in samples after reduction in incubation time were not found. Conclusions. The most economical method of DNA isolation from bees’ biological material is 20% solution of «Chelex-100» ion exchange resin with the duration of the incubation period of 180 minutes. It should also be noted that the best results can be obtained from exuviae, selected immediately after the queen’s exit from bowl, that reduces the likelihood of DNA molecules destruction under the influence of nucleases activation, but not later than 12 hours from release using the technology of isolated obtain of queens.

Adsorption and Recovery of Dissolved Organic Phosphorus and Nitrogen by Mixed-Bed Ion-Exchange Resin

2003 ◽  
Vol 67 (3) ◽  
pp. 889 ◽  
Author(s):  
Jacques L. Langlois ◽  
Dale W. Johnson ◽  
Guy R. Mehuys
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Organic Phosphorus ◽  
Ion Exchange Resin ◽  
Dissolved Organic Phosphorus ◽  
Mixed Bed Ion Exchange

Dissolution Kinetics of Phlogopite. II. Open System Using an Ion-Exchange Resin

1981 ◽  
Vol 29 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Charles V. Clemency
Keyword(s):  
Ion Exchange ◽  
Open System ◽  
Exchange Resin ◽  
Dissolution Kinetics ◽  
Ion Exchange Resin ◽  
Kinetics Of

Recycling of Wastewater Containing Iron-Complex Cyanides Using UV Photodecomposition and UV Ozone Oxidation in Combination with an Ion-Exchange Resin Method

2005 ◽  
Vol 78 (3) ◽  
pp. 512-518 ◽  
Author(s):  
Hiromutsu Wada ◽  
Kazuko Yanaga ◽  
Yasuhiro Kuroda ◽  
Sergio Hanela ◽  
Yoshio Hirayama
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Iron Complex ◽  
Ozone Oxidation ◽  
Uv Ozone ◽  
Resin Method

Chromatography of chlorinated biphenyls on an ion-exchange resin

1977 ◽  
Vol 49 (6) ◽  
pp. 764-766 ◽  
Author(s):  
Toshihiko. Hanai ◽  
Harold F. Walton
Keyword(s):  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin
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