[2] Separation and purification of cyclic nucleotides by ion-exchange resin column chromatography

Author(s):  
G. Schultz ◽  
E. Böhme ◽  
J.G. Hardman
1967 ◽  
Vol 50 (4) ◽  
pp. 783-786
Author(s):  
Eugene A Breatjlt

Abstract The metals Mn, Mg, Co, Cu, and Zn were separated by using an anion exchange resin column and progressively less concentrated IIC1 solution as eluting agent. The position of Mg on an anion exchange resin, not previously reported in the literature, was determined. It has been shown that by using two different ion exchange resin columns, simple hydrochloric acid solutions as eluants, and polarographic or flame photometric determinations, 12 metals can be separately estimated in an aqueous solution containing about 100 /μg of each. It is recommended that the study of ion exchange resin separation of metals found in plants be continued.


Polyhedron ◽  
2020 ◽  
Vol 188 ◽  
pp. 114694
Author(s):  
Na Xia ◽  
Wenjing Wan ◽  
Siming Zhu ◽  
Huan Wang ◽  
Kouame Ally

2010 ◽  
Vol 115 (1) ◽  
pp. 324-329 ◽  
Author(s):  
Noureddine Charef ◽  
Lekhmici Arrar ◽  
Afaf Lamaaoui ◽  
Hiba Boudjellal ◽  
Abderrahmane Baghiani ◽  
...  

1959 ◽  
Vol 37 (3) ◽  
pp. 405-416 ◽  
Author(s):  
Strathearn Wilson ◽  
David B. Smith

Horse globin has been fractionated into two components by stepwise addition of acid acetone to an acidified globin solution and by gradient elution with urea from a carboxylic-type ion exchange resin at low pH.The components obtained by both methods are similar to those prepared earlier by electrophoresis. They have similar molecular weights (about 16,000) and appear to be present in nearly equal amounts. One has the N-terminal sequence valyl-leucyl- and the other valyl-glutamyl-. They differ electrokinetically and in other properties.


Biomolecules ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1601 ◽  
Author(s):  
Laura Mitrea ◽  
Loredana Florina Leopold ◽  
Cosmina Bouari ◽  
Dan Cristian Vodnar

In the present work, was investigated the separation and purification procedure of the biogenic 1,3-propanediol (1,3-PD), which is a well-known valuable compound in terms of bio-based plastic materials development. The biogenic 1,3-PD was obtained as a major metabolite through the glycerol fermentation by Klebsiella pneumoniae DSMZ 2026 and was subjected to separation and purification processes. A strong acidic ion exchange resin in H+ form was used for 1,3-PD purification from the aqueous solution previously obtained by broth flocculation. The eluent volume was investigated considering the removal of the secondary metabolites such as organic acids (acetic, citric, lactic, and succinic acids) and 2,3-butanediol (2,3-BD), and unconsumed glycerol. It was observed that a volume of 84 mL of ethanol 75% loaded with a flow rate of 7 mL/min completely remove the secondary metabolites from 10 mL of concentrated fermented broth, and pure biogenic 1,3-PD was recovered in 128 mL of the eluent.


2002 ◽  
Vol 2 ◽  
pp. 122-130 ◽  
Author(s):  
Mark E. Fenn ◽  
Mark A. Poth ◽  
Michael J. Arbaugh

Measurement of ionic deposition in throughfall is a widely used method for measuring deposition inputs to the forest floor. Many studies have been published, providing a large database of throughfall deposition inputs to forests. However, throughfall collection and analysis is labor intensive and expensive because of the large number of replicate collectors needed and because sample collection and chemical analyses are required on a stochastic precipitation event-based schedule. Therefore we developed and tested a throughfall collector system using a mixed bed ion exchange resin column. We anticipate that this method will typically require only one to three samplings per year. With this method, bulk deposition and bulk throughfall are collected by a funnel or snow tube and ions are retained as the solution percolates through the resin column. Ions retained by the resin are then extracted in the same column with 2N KCl and analyzed for nitrate and ammonium. Deposition values in throughfall from conventional throughfall solution collectors and colocated ion exchange samplers were not significantly different during consecutive 3- and 4-month exposure periods at a high (Camp Paivika; >35 kg N ha-1year-1) and a low deposition (Barton Flats; 5–9 kg N ha-1year-1) site in the San Bernardino Mountains in southern California. N deposition in throughfall under mature pine trees at Camp Paivika after 7 months of exposure was extremely high (87 and 92 kg ha-1based on the two collector types) compared to Barton Flats (11 and 13 kg ha-1). A large proportion of the N deposited in throughfall at Camp Paivika occurred as fog drip, demonstrating the importance of fog deposition as an input source of N at this site. By comparison, bulk deposition rates in open areas were 5.1 and 5.4 kg ha-1at Camp Paivika based on the two collector types, and 1.9 and 3.0 kg ha-1at Barton Flats.


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