THE DETERMINATION OF TERMINAL PROTEIN-BOUND FUCOSE

1965 ◽  
Vol 43 (11) ◽  
pp. 1807-1811 ◽  
Author(s):  
G. Gyorky ◽  
J. C. Houck
Keyword(s):  
Protein Degradation ◽  
Spectrophotometric Determination ◽  
Trichloroacetic Acid ◽  
Degradation Products ◽  
Dilute Acid ◽  
Terminal Protein ◽  
Residual Protein ◽  
Color Yield ◽  
Fucose Content

The spectrophotometric determination of protein-bound fucose is badly compromised by spurious chromagens developed from protein degradation products. To minimize the contribution of these spurious products to the color yield of fucose, the glycoprotein was partially hydrolyzed in dilute acid, thus releasing the terminal carbohydrate from the protein moieties, and the residual protein was removed with trichloroacetic acid. That the fucose content of this supernatant was real was confirmed by paper chromatography and spectral studies.The spurious chromagens were shown to result from the interaction of protein degradation products and galactose.

Spectrophotometric Determination of Isocarboxazid Bulk Drug and Tablets by Reaction with/7-Dimethylaminocinnamaldehyde

1988 ◽  
Vol 71 (6) ◽  
pp. 1134-1136
Author(s):  
Michael E El-Kommos
Keyword(s):  
Regression Analysis ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Trichloroacetic Acid ◽  
Excellent Correlation ◽  
Electrophilic Attack ◽  
Reagent Ratio ◽  
Bulk Drug ◽  
Methanolic Medium

Abstract A Spectrophotometric Method Is Described For The Determination Of Isocarboxazid. The Method Is Based On The Reaction Of The Drug With p-Dimethylaminocinnamaldehyde In The Presence Of Trichloroacetic Acid In A Methanolic Medium To Produce A Very Intense Red Chromophore (ℷmax = 500 Nm, Emax= 1.05 X 105). The Reaction Is Proposed To Proceed Via Electrophilic Attack At The C-4 Position Of The Isoxazole Nucleus. Job's Plot Indicated A 1:1 Drug-To-Reagent Ratio. Regression Analysis Of Beer's Plot Showed Excellent Correlation (R = 0.9996) In The Concentration Range 0.25-2.10 Fig Isocarboxazid/Ml. The Developed Color Is Stable For At Least 12 H. Results Of Analyses Of Bulk Drug And Tablets By The Proposed Method Are Comparable To Those For Usp Xxi Methods.

Second Derivative Spectrophotometric Determination of Acetaminophen and Phenacetin in Presence of Their Degradation Products

1986 ◽  
Vol 69 (4) ◽  
pp. 608-611
Author(s):  
Mohamed A Korany ◽  
Mona Bedair ◽  
Hoda Mahgoub ◽  
Mahmoud A Elsayed
Keyword(s):  
Spectrophotometric Determination ◽  
Degradation Products ◽  
Spectral Response ◽  
Accurate Method ◽  
Second Derivative ◽  
The Mean ◽  
The Stability

Abstract A rapid and accurate method for determining acetaminophen and phenacetin in presence of their degradation products is presented. Solutions of these drugs in 0.1N HC1 were analyzed by measuring their second derivative spectral response at 295 nm where the degradation products do not interfere. The mean percent recoveries for mixtures of acetaminophen and/or phenacetin with the corresponding degradation products were 100.2 ± 0.6 and 100.6 ± 1.1, respectively. The method can be used for assessing the stability of the 2 drugs. The proposed method is also applied to the determination of acetaminophen in tablets and syrups.

Flameless Atomic Absorption Spectrophotometric Determination of Vendex, an Organic Tin Miticide, in Apples, Oranges, and Tea Leaves

1979 ◽  
Vol 62 (4) ◽  
pp. 764-768
Author(s):  
Mitsuaki Sano ◽  
Masako Furukawa ◽  
Miyako Kourai ◽  
Isao Tomita
Keyword(s):  
Acetic Acid ◽  
Atomic Absorption ◽  
Silica Gel ◽  
Spectrophotometric Determination ◽  
Ethyl Ether ◽  
Degradation Products ◽  
Tea Leaves ◽  
Tin Compounds ◽  
Average Recovery

Abstract A method is described for the determination of an organic tin insecticide, Vendex or hexakis(2-methyl-2-phenylpropyl)distannoxane (HMPD). Tin compounds are extracted from the sample homogenate with ethyl ether containing 1% acetic acid. HMPD and its degradation products are separated through a silica gel column, and analyzed by an atomic absorption spectrophotometer coupled with a heated graphite atomizer. By this technique, as little as 5 ng HMPD and its degradation products can be determined with an average recovery of 67–96%.

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