Spectrophotometric Determination of Isocarboxazid Bulk Drug and Tablets by Reaction
with/7-Dimethylaminocinnamaldehyde
Abstract A Spectrophotometric Method Is Described For The Determination Of Isocarboxazid. The Method Is Based On The Reaction Of The Drug With p-Dimethylaminocinnamaldehyde In The Presence Of Trichloroacetic Acid In A Methanolic Medium To Produce A Very Intense Red Chromophore (ℷmax = 500 Nm, Emax= 1.05 X 105). The Reaction Is Proposed To Proceed Via Electrophilic Attack At The C-4 Position Of The Isoxazole Nucleus. Job's Plot Indicated A 1:1 Drug-To-Reagent Ratio. Regression Analysis Of Beer's Plot Showed Excellent Correlation (R = 0.9996) In The Concentration Range 0.25-2.10 Fig Isocarboxazid/Ml. The Developed Color Is Stable For At Least 12 H. Results Of Analyses Of Bulk Drug And Tablets By The Proposed Method Are Comparable To Those For Usp Xxi Methods.