Polyol–protein interactions. Thermodynamical evidence for a selective solvation of glycerol and hexitols by aqueous β-lactoglobulin
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Apparent specific heat capacities and volumes of the protein β-lactoglobulin have been determined under a variety of solute–solvent and solvent–cosolvent compositions. These quantities were derived from measurements of volumetric specific heats and densities with a flow microcalorimeter and flow densimeter. The effect of homologous polyols on the isothermal denaturation of the protein by urea at 25 °C was investigated. The results suggest that polyol binding on the protein is sensitive to structural effects, maximum discrimination being exerted in favor of glycerol and its doubles, the hexitols.
2013 ◽
Vol 58
(3)
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pp. 625-633
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2010 ◽
Vol 55
(6)
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pp. 2267-2270
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2013 ◽
Vol 115
(3)
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pp. 2071-2077
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2007 ◽
Vol 16
(07n08)
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pp. 1912-1916
2005 ◽
Vol 79
(2)
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pp. 283-287
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