The nephritogenic glycopeptide from rat glomerular basement membrane: synthesis of α-D-glucopyranosylamine derivatives

1980 ◽  
Vol 58 (23) ◽  
pp. 2600-2603 ◽  
Author(s):  
Tadahiro Takeda ◽  
Yoshihiro Sugiura ◽  
Yukio Ogihara ◽  
Seiichi Shibata
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Nmr Spectra ◽  
Protecting Groups ◽  
Membrane Synthesis ◽  
Basement Membrane Synthesis ◽  
C Nmr

N-(L-β-aspartyl)-α-D-glucopyranosylamine has been prepared, as a model of a corresponding derivative possibly present in glomerular basement membrane of rats, by the condensation of α-ethyl benzyloxycarbonyl-L-aspartate with 2,3,4,6-tetra-O-acetyl-α-D-glucopyranosylamine in the presence of diethylphosphorocyanidate. This was followed by hydrogenolysis, de-O-acetylation, and deethoxylation of the resulting 2,3,4,6-tetra-O-acetyl-N-(α-ethyl benzyloxycarbonyl-L-β-aspartyl)-α-D-glucopyranosylamine to remove the protecting groups. The 13C nmr spectra of the corresponding glycosylamine derivatives are analyzed and discussed.

scholarly journals The prognostic significance of type IV collagen expression in colorectal carcinomas

2003 ◽  
Vol 11 (2) ◽  
pp. 65-70 ◽  
Author(s):  
Attila Fenyvesi
Keyword(s):  
Colorectal Cancer ◽  
Basement Membrane ◽  
Type Iv Collagen ◽  
Histological Grade ◽  
Colorectal Carcinomas ◽  
Membrane Synthesis ◽  
Stage Of Disease ◽  
Collagen Expression ◽  
Type Iv ◽  
Basement Membrane Synthesis

BACKGROUND: Breakdown of basement membrane is believed to be an essential step for tumor invasion and metastasis. The interaction between tumor cells and extracellular matrix can also result in induction of basement membrane synthesis by tumor and stromal cells. The aim of this study was to investigate distribution patterns of type IV collagen expression at the basement membrane in the tumor tissue of colorectal carcinomas by immunohistochemical staining. The degree of expression of type IV collagen correlated with classical clinicopathologic prognostic factors and their potential relationship with patients' prognosis. PATIENTS AND METHODS: This study included 40 patients who underwent curative resection of colorectal cancer at the Department of Surgery in the General Hospital Senta with complete follow-up for 5 years or until death. The identification of basement membrane was performed immunohistochemically using monoclonal antibodies to collagen IV. The basement membrane synthesis at the invasive front of colorectal cancer was semiquantitatively assessed as mild, moderate or severe. RESULTS: The deposition of basement membrane type of collagen IV had a statistically significant correlation with the stage of disease and histological grade of tumor with tendency of lesser synthesis of basement membrane in the advanced stage of disease and poorer histological grade of tumor. There were no significant correlations between intratumoral basement membrane synthesis and sex and age of patients, and localization and histological type of tumor. Five-year survival rates in patients with mild synthesis of basement membrane in colorectal tumor were significantly lower than in patients with severe expression of basement membrane. The basement membrane synthesis in colorectal cancer was a prognostic factor with significance behind stage of disease and histological grade of tumor by univariate Cox hazard-model. Multivariate Cox analysis verified that explicit synthesis of basement membrane showed a trend toward better prognosis, but it was not statistically significant. CONCLUSION Immunohistochemical staining for type IV collagen is an important additional technique with prognostic value.

Basement Membrane Synthesis, Accumulation and Turnover in vitro

1975 ◽  
pp. 357-361 ◽  
Author(s):  
R.R. MINOR ◽  
C.C. CLARK ◽  
T.R. KOSZALKA ◽  
R.L. BRENT ◽  
N.A. KEFALIDES
Keyword(s):  
Basement Membrane ◽  
Membrane Synthesis ◽  
Basement Membrane Synthesis

scholarly journals Epithelial HVEM promotes basement membrane synthesis and intraepithelial T cell survival and migration

2020 ◽  
Author(s):  
Daisuke Takahashi ◽  
Qingyang Wang ◽  
Goo-Young Seo ◽  
Jr-wen Shui ◽  
Zbigniew Mikulski ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Basement Membrane ◽  
Collagen Iv ◽  
Membrane Synthesis ◽  
Basement Membrane Synthesis ◽  
And Migration ◽  
Membrane Components ◽  
T Cell Survival ◽  
Lymphocyte Populations

SummaryIntraepithelial T cells (IET) provide continuous surveillance of the intestinal epithelium, but little was known about how epithelial-derived signals regulate the IET population. We show that epithelial expression of the herpes virus entry mediator (HVEM), a member of the TNF receptor superfamily (TNFRSF), maintained the survival of small intestine IET, especially innate-like TCRαβ+ cells lacking CD4 and CD8β. Patrolling movement of all CD8α+ IET also was impaired in the absence of HVEM. HVEM-deficient epithelial cells exhibited downregulation of synthesis of basement membrane components, including collagen IV. Collagen IV supported IET survival in vitro via interactions with β1 integrins expressed by the IET; absence of β1 integrins decreased some IET subsets. Therefore, these data define a circuit whereby epithelial cells regulate intestine resident T lymphocyte populations through basement membrane synthesis.

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