scholarly journals Glomerular basement membrane synthesis and serum concentration of type IV collagen in streptozotocin-diabetic rats

Diabetologia ◽  
1984 ◽  
Vol 26 (2) ◽  
Author(s):  
Ch. Hasslacher ◽  
R. Reichenbacher ◽  
F. Gechter ◽  
R. Timpl
Keyword(s):  
Basement Membrane ◽  
Serum Concentration ◽  
Glomerular Basement Membrane ◽  
Type Iv Collagen ◽  
Diabetic Rats ◽  
Membrane Synthesis ◽  
Type Iv ◽  
Basement Membrane Synthesis ◽  
Streptozotocin Diabetic Rats

scholarly journals The prognostic significance of type IV collagen expression in colorectal carcinomas

2003 ◽  
Vol 11 (2) ◽  
pp. 65-70 ◽  
Author(s):  
Attila Fenyvesi
Keyword(s):  
Colorectal Cancer ◽  
Basement Membrane ◽  
Type Iv Collagen ◽  
Histological Grade ◽  
Colorectal Carcinomas ◽  
Membrane Synthesis ◽  
Stage Of Disease ◽  
Collagen Expression ◽  
Type Iv ◽  
Basement Membrane Synthesis

BACKGROUND: Breakdown of basement membrane is believed to be an essential step for tumor invasion and metastasis. The interaction between tumor cells and extracellular matrix can also result in induction of basement membrane synthesis by tumor and stromal cells. The aim of this study was to investigate distribution patterns of type IV collagen expression at the basement membrane in the tumor tissue of colorectal carcinomas by immunohistochemical staining. The degree of expression of type IV collagen correlated with classical clinicopathologic prognostic factors and their potential relationship with patients' prognosis. PATIENTS AND METHODS: This study included 40 patients who underwent curative resection of colorectal cancer at the Department of Surgery in the General Hospital Senta with complete follow-up for 5 years or until death. The identification of basement membrane was performed immunohistochemically using monoclonal antibodies to collagen IV. The basement membrane synthesis at the invasive front of colorectal cancer was semiquantitatively assessed as mild, moderate or severe. RESULTS: The deposition of basement membrane type of collagen IV had a statistically significant correlation with the stage of disease and histological grade of tumor with tendency of lesser synthesis of basement membrane in the advanced stage of disease and poorer histological grade of tumor. There were no significant correlations between intratumoral basement membrane synthesis and sex and age of patients, and localization and histological type of tumor. Five-year survival rates in patients with mild synthesis of basement membrane in colorectal tumor were significantly lower than in patients with severe expression of basement membrane. The basement membrane synthesis in colorectal cancer was a prognostic factor with significance behind stage of disease and histological grade of tumor by univariate Cox hazard-model. Multivariate Cox analysis verified that explicit synthesis of basement membrane showed a trend toward better prognosis, but it was not statistically significant. CONCLUSION Immunohistochemical staining for type IV collagen is an important additional technique with prognostic value.

scholarly journals Glomerular Basement Membrane Selective Permeability in Short-term Streptozotocin-induced Diabetic Rats

2000 ◽  
Vol 1 (1) ◽  
pp. 19-30 ◽  
Author(s):  
Michele Doucet ◽  
Irene Londoño ◽  
Amparo Gómez-Pascual ◽  
Moise Bendayan
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Type Iv Collagen ◽  
Serum Proteins ◽  
Diabetic Rats ◽  
Glomerular Permeability ◽  
Structural Alterations ◽  
Early Stages ◽  
Type Iv ◽  
Basement Membrane Thickening

In diabetes, the glomerular basement membrane undergoes thickening and structural alterations with loss of glomerular permselectivity properties. However, the onset of the alterations at early phases of diabetes is unclear. Aiming to determine the functional and structural alterations of the glomerular wall in the early stages of diabetes, we have studied the distribution of endogenous circulating albumin and type IV collagen in the glomerular basement membrane, using the immunocytochemical approach. The streptozotocin-injected hyperglycemic rat was our animal model. Renal tissues were examined after 10 days, 2, 4 and 6 months of hyperglycemia. Upon immunogold labelings, changes in the glomerular permeability to endogenous albumin were found altered as early as upon ten days of hyperglycemia. In contrast, no structural modifications were detected at this time point. Indeed, glomerular basement membrane thickening and an altered type IV collagen labeling distribution were only observed after four months of hyperglycemia, suggesting that functional alterations take place early in diabetes prior to any structural modification. In order to evaluate the reversibility of the glomerular alterations, two-month-old diabetic animals were treated with insulin. These animals showed a significant restoring of their glomerular permselectivity. Our results suggest a link between glycemic levels and alteration of glomerular permeability in early stages of diabetes, probably through high levels of glycated serum proteins.

IDENTIFICATION OF ??3, ??4, AND ??5 CHAINS OF TYPE IV COLLAGEN AS ALLOANTIGENS FOR ALPORT POSTTRANSPLANT ANTI-GLOMERULAR BASEMENT MEMBRANE ANTIBODIES

2000 ◽  
Vol 69 (4) ◽  
pp. 679-684 ◽  
Author(s):  
Raghu Kalluri ◽  
Adriana Torre ◽  
Charles F. Shield ◽  
Eric D. Zamborsky ◽  
Michelle C. Werner ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Type Iv Collagen ◽  
Type Iv

scholarly journals RETRACTED: Production and Characterization of Recombinant Rat Non-collagen Domain of α3 Chain of Type IV Collagen α3 (IV) NC1 Antigen

2016 ◽  
Vol 12 (24) ◽  
pp. 98
Author(s):  
Afsana Munni
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Type Iv Collagen ◽  
Kidney Diseases ◽  
Cell Epitope ◽  
Affinity Column ◽  
Igg Antibody ◽  
Amino Terminal ◽  
Type Iv ◽  
Collagenous Domain

Glomerulonephritis disease is characterized by inflammation of glomeruli or small blood vessels in the kidney which causes kidney diseases. Glomerulonephritis disease deposits the anti-GBM auto antibody in the glomerular basement membrane. The type IV collagen is the main component of glomerular basement membrane that has α3 chain of type (IV) collagen of non-collagenous domain which contains N-terminal 7S domain, a triple helical collagenous domain, and a C- terminal non-collagenous glomerular domain (NC1). The amino terminal of α3 (IV) NC1 that induces the experimental autoimmuno glomerulonephritis (EAG) in rat model has been identified. The recombinant rat α3 (IV)NC1 antigen has nine amino acid span that is consistent with antibody or T cell epitope which is induced in EAG. The research is carried out on the recombinant rat α3 (IV) NC1 production, purification, quantification, and characterization. The circulation of Anti-GBM antibody in glomerular basement membrane can be measured by the ELISA assay. In addition, the recombinant rat antigen is secreted in HEK293 cell supernatant which is purified by Anti-FLAG M2 monoclonal IgG antibody affinity column. In addition, it is characterized and quantified by SDS-PAGE gel electrophoresis and Western blotting techniques.

scholarly journals The prevalence and immunological features of anti-glomerular basement membrane antibody in patients with HIV

2020 ◽  
Author(s):  
Wenjing Wang ◽  
Xiao-yu Jia Jia ◽  
Zhao Cui ◽  
Yan Chen ◽  
Wei Wang ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Type Iv Collagen ◽  
Solid Phase ◽  
Chimeric Protein ◽  
Kidney Injury ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hiv Viral Load ◽  
Hiv Patients ◽  
Type Iv

Abstract Background: Anti-glomerular basement membrane disease (GBM) is a kind of chronic autoimmune disease caused by the deposition of circulating anti-GBM antibodies. Non-collagen region of α3 chain of type IV collagen (α3(IV)NC1) is one of the main target antigens. And on α3, EA and EB are the most classical antigen epitopes. It has been reported that anti-GBM antibodies can be detected in HIV patients, but its immunological characteristics are still unclear. In this study, the immunological characteristics of the target antigens were clarified. Methods: Total 93 HIV patients and 20 healthy volunteers were selected in Beijing Youan Hospital from 2017 to 2018. Recombinant human α1-α5(IV)NC1, chimeric protein EA and EB were used as solid phase antigens. Enzyme-linked immunosorbent assay was employed to measure concentrations and subtypes of serum IgG autoantibodies specifically against GBM.Results: Five out of the 93 patients with HIV had low to moderate levels of anti-GBM antibodies. However, these patients presented with no clinical manifestation of any kidney injury or pulmonary hemorrhages. Compared with HIV patients with negative antibodies, there were no significant differences in gender, age, CD4+T cell count and HIV viral load. All sera of five patients recognized non-collagenous domain1 (NC1) of alpha 3 chain of type IV collagen [(α3(IV)NC1] as classic anti-GBM patients, followed by α5(IV)NC1. The antibodies against α3(IV)NC1 were IgG3 predominant, but did not react with either of the classic epitopes on α3 (EA and EB).Conclusion: These data suggest a distinct immunological profile of anti-GBM antibodies in patients with HIV, and might explain the non-pathogenic features of HIV associated anti-GBM antibodies.

A Case of IgA Nephropathy with Split Reduction of α5(V) Chain of Type IV Collagen in Glomerular Basement Membrane

Nephron ◽  
1998 ◽  
Vol 80 (4) ◽  
pp. 482-483 ◽  
Author(s):  
Shigeru Horita ◽  
Kosaku Nitta ◽  
Kazuho Honda ◽  
Hideo Kobayashi ◽  
Keiko Uchida ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Iga Nephropathy ◽  
Glomerular Basement Membrane ◽  
Type Iv Collagen ◽  
Type Iv

The nephritogenic glycopeptide from rat glomerular basement membrane: synthesis of α-D-glucopyranosylamine derivatives

1980 ◽  
Vol 58 (23) ◽  
pp. 2600-2603 ◽  
Author(s):  
Tadahiro Takeda ◽  
Yoshihiro Sugiura ◽  
Yukio Ogihara ◽  
Seiichi Shibata
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Nmr Spectra ◽  
Protecting Groups ◽  
Membrane Synthesis ◽  
Basement Membrane Synthesis ◽  
C Nmr

N-(L-β-aspartyl)-α-D-glucopyranosylamine has been prepared, as a model of a corresponding derivative possibly present in glomerular basement membrane of rats, by the condensation of α-ethyl benzyloxycarbonyl-L-aspartate with 2,3,4,6-tetra-O-acetyl-α-D-glucopyranosylamine in the presence of diethylphosphorocyanidate. This was followed by hydrogenolysis, de-O-acetylation, and deethoxylation of the resulting 2,3,4,6-tetra-O-acetyl-N-(α-ethyl benzyloxycarbonyl-L-β-aspartyl)-α-D-glucopyranosylamine to remove the protecting groups. The 13C nmr spectra of the corresponding glycosylamine derivatives are analyzed and discussed.

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