Purification and properties of NAD(P)-independent polyol dehydrogenase complex from the plasma membrane ofGluconobacter oxydans
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Gluconobacter oxydans rapidly oxidizes many different polyhydroxy alcohols (polyols). Polyol oxidations are catalyzed by constitutively synthesized membrane-bound dehydrogenases directly linked to the electron transport chain. A polyol-oxidizing enzyme was isolated from the membranes of G. oxydans and tested for its ability to oxidize various substrates. The enzyme was composed of three subunits: a 67 kDa catalytic unit, a 46 kDa c-type cytochrome, and a 15 kDa subunit. The enzyme oxidized compounds containing three or more hydroxyl groups but did not oxidize mono-, di-, or cyclic alcohols; aldehydes; carboxylic acids; or mono- or di-saccharides. Therefore, we propose this enzyme be considered a polyol dehydrogenase.
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2010 ◽
Vol 56
(10)
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pp. 803-808
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1972 ◽
Vol 247
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pp. 3001-3007
1963 ◽
Vol 238
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pp. 4052-4057