Ferrous iron oxidation in moderately thermophilic acidophile Sulfobacillus sibiricus N1T

2010 ◽  
Vol 56 (10) ◽  
pp. 803-808 ◽  
Author(s):  
Tatiana Y. Dinarieva ◽  
Anna E. Zhuravleva ◽  
Oksana A. Pavlenko ◽  
Iraida A. Tsaplina ◽  
Alexander I. Netrusov
Keyword(s):  
Ferrous Iron ◽  
High Performance ◽  
Iron Oxidation ◽  
Early Exponential Phase ◽  
Moderately Thermophilic ◽  
Terminal Oxidases ◽  
Cytochrome Bo ◽  
Ferrous Iron Oxidation ◽  
Transport Chain ◽  
Membrane Bound

The iron-oxidizing system of a moderately thermophilic, extremely acidophilic, gram-positive mixotroph, Sulfobacillus sibiricus N1T, was studied by spectroscopic, high-performance liquid chromatography and inhibitory analyses. Hemes B, A, and O were detected in membranes of S. sibiricus N1T. It is proposed that the electron transport chain from Fe2+ to O2 is terminated by 2 physiological oxidases: aa3-type cytochrome, which dominates in the early-exponential phase of growth, and bo3-type cytochrome, whose role in iron oxidation becomes more prominent upon growth of the culture. Both oxidases were sensitive to cyanide and azide. Cytochrome aa3 was more sensitive to cyanide and azide, with Ki values of 4.1 and 2.5 µmol·L–1, respectively, compared with Ki values for cytochrome bo3, which were 9.5 µmol·L–1 for cyanide and 7.0 µmol·L–1 for azide. This is the first evidence for the participation of a bo3-type oxidase in ferrous iron oxidation. The respiratory chain of the mixotroph contains, in addition to the 2 terminal oxidases, a membrane-bound cytochrome b573.

Kinetics of Ferrous Iron Oxidation by Moderately Thermophilic Microorganisms

2007 ◽  
Vol 20-21 ◽  
pp. 517-520
Author(s):  
Pablo S. Pina ◽  
V.A. Leão ◽  
C.A. Silva ◽  
S.N. Medrício ◽  
J. Frenay
Keyword(s):  
Ferrous Iron ◽  
Iron Oxidation ◽  
Thermophilic Microorganisms ◽  
Moderately Thermophilic ◽  
Ferrous Iron Oxidation ◽  
Kinetics Of

Resistance of Moderately Thermophilic Acidophilic Microorganisms to Ferric Iron Ions

2017 ◽  
Vol 262 ◽  
pp. 471-475
Author(s):  
Aleksander Bulaev
Keyword(s):  
Ferrous Iron ◽  
Ferric Iron ◽  
Iron Oxidation ◽  
Iron Concentration ◽  
Iron Ions ◽  
Moderately Thermophilic ◽  
Ferrous Iron Oxidation ◽  
Low Concentrations ◽  
The Media ◽  
High Concentrations

Resistance of microorganisms predominating in biohydrometallurgical processes including bacteria of the genus Sulfobaсillus and archaea of the genus Acidiplasma to ferric iron ions was studied. Capabilities of the strains for growth and ferrous iron oxidation in the media containing high concentrations of ferric iron ions (of 250 to 1000 mM) were evaluated. Ferric iron ions significantly inhibited oxidative activity and growth of the studied microorganisms. It was revealed that bacteria of the genus Sulfobacillus were not able to oxidize ferrous iron actively when ferric iron concentration exceeded 500 mM, whereas archaea of the genus Acidiplasma completely oxidized ferrous iron in the medium containing 1000 mM of Fe3+. Growth of the microorganisms was inhibited by relatively low concentrations of ferric iron. Microorganisms did not grow in the medium containing more than 750 mM of Fe3+ and cells of all studied strains lysed in presence of high concentrations of ferric iron. It was shown, that archaea of the genus Acidiplasma of the family Ferroplasmaceae were more resistant to high concentrations of ferric iron than bacteria of the genus Sulfobacillus. The results obtained are important for understanding of the regularities of the formation of microbial communities performing technological processes.

Ferrous Iron Oxidation in Packed-Bed Reactors at Elevated Temperatures

2015 ◽  
Vol 1130 ◽  
pp. 226-229 ◽  
Author(s):  
Aleksander Bulaev
Keyword(s):  
Ferrous Iron ◽  
Elevated Temperatures ◽  
Iron Oxidation ◽  
Packed Bed ◽  
Thermophilic Microorganisms ◽  
Packed Bed Reactors ◽  
Moderately Thermophilic ◽  
Oxidation Rates ◽  
Ferrous Iron Oxidation ◽  
In Series

The ferrous iron oxidation by mixed culture of moderately thermophilic microorganisms (Sulfobacillus thermosulfidooxidansSh 10-1 andAcidiplasmaMBA-1) was investigated in continuous experiments in three packed-bed reactors connected in series at temperature 55°C, and a pH of 1.0. Two solutions were used in the experiments. The first one contained (g L-1) 59 Fe2+, the second one contained (g L-1) 59 Fe2+, 16 Fe3+, 2 Cu2+, 2 Zn2+. The hydraulic retention time was 120 hours. Iron oxidation rates in the experiment with the first solution were 0.5, 0.35, and 0.2 g L-1h-1in first, second and third reactor, respectively. The oxidation rates in the experiment with the second solution were 0.3, 0.2, and 0.185 g L-1h-1in first, second and third reactor, respectively. Iron oxidation efficiencies in the experiments with the first and second solutions were 77% and 47%. Stable continuous iron oxidation at high temperature was successfully demonstrated, but further investigations are required for improving the rate and efficiencies of oxidation.

scholarly journals A kinetic model of ferrous iron oxidation by Acidithiobacillus ferrooxidans in a batch culture

2005 ◽  
Vol 11 (2) ◽  
pp. 59-62 ◽  
Author(s):  
Dragisa Savic ◽  
Miodrag Lazic ◽  
Vlada Veljkovic ◽  
Miroslav Vrvic
Keyword(s):  
Kinetic Model ◽  
Acidithiobacillus Ferrooxidans ◽  
Ferrous Iron ◽  
Bubble Column ◽  
Iron Oxidation ◽  
Yield Coefficient ◽  
Transfer Rates ◽  
Ferrous Iron Oxidation ◽  
Menten Kinetic ◽  
Kinetics Of

The batch oxidation kinetics of ferrous iron by Acidithiobacillus ferrooxidans were examined at different oxygen transfer rates and pH in an aerated stirred tank and a bubble column. The microbial growth, oxygen consumption rate and ferrous and ferric iron were monitored during the biooxidation. A kinetic model was established on the basis of the Michaelis-Menten kinetic equation for bacterial growth and the constants estimated from experimental data (maximum specific growth rate 0.069 h-1, saturation constant 2.9 g/dm3, and biomass yield coefficient based on ferrous iron 0.003 gd.w./gFe). Values calculated from the model agreed well with the experimental ones regardless of the bioreactor type and pH conditions.

Iron targeted transcriptome study draws attention to novel redox protein candidates involved in ferrous iron oxidation in “Ferrovum” sp. JA12

2018 ◽  
Vol 169 (10) ◽  
pp. 618-627 ◽  
Author(s):  
Sophie R. Ullrich ◽  
Anja Poehlein ◽  
Gloria Levicán ◽  
Martin Mühling ◽  
Michael Schlömann
Keyword(s):  
Ferrous Iron ◽  
Iron Oxidation ◽  
Redox Protein ◽  
Ferrous Iron Oxidation

The kinetics of ferrous-iron oxidation by Leptospirillum ferriphilum in continuous culture: The effect of temperature

2009 ◽  
Vol 46 (2) ◽  
pp. 161-168 ◽  
Author(s):  
Tunde V. Ojumu ◽  
Geoffrey S. Hansford ◽  
Jochen Petersen
Keyword(s):  
Continuous Culture ◽  
Ferrous Iron ◽  
Iron Oxidation ◽  
Effect Of Temperature ◽  
Leptospirillum Ferriphilum ◽  
Ferrous Iron Oxidation ◽  
Kinetics Of

scholarly journals Increasing cell concentration does not affect specific ferrous iron oxidation rate in a continuously stirred tank bioreactor

2018 ◽  
Vol 181 ◽  
pp. 189-194
Author(s):  
Naomi J. Boxall ◽  
Ka Yu Cheng ◽  
Chris A. du Plessis ◽  
David Collinson ◽  
Christina Morris ◽  
...  
Keyword(s):  
Oxidation Rate ◽  
Ferrous Iron ◽  
Cell Concentration ◽  
Iron Oxidation ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Ferrous Iron Oxidation

Effects of the oxygen transfer rate on ferrous iron oxidation by Thiobacillus ferrooxidans

1998 ◽  
Vol 23 (7-8) ◽  
pp. 427-431 ◽  
Author(s):  
D.S Savić ◽  
V.B Veljković ◽  
M.L Lazić ◽  
M.M Vrvić ◽  
J.I Vučetić
Keyword(s):  
Oxygen Transfer ◽  
Ferrous Iron ◽  
Transfer Rate ◽  
Thiobacillus Ferrooxidans ◽  
Iron Oxidation ◽  
Oxygen Transfer Rate ◽  
Ferrous Iron Oxidation
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