Metabolism of [3H]noradrenaline by the isolated rat uterus

The metabolism of [3H]noradrenaline by uterine horns from estrous, ovariectomized, and ovariectomized and estrogen-treated rats was explored. Uterine strips are able to take up [3H]noradrenaline and store it for up to 85 min after the isotope incubation. The major compounds retained in the tissue from rats either ovariectomized or during estrus were [3H]noradrenaline and 3H-labelled O-methylated deaminate metabolites. The [3H]3,4-dihydroxyphenylglycol ([3H]DOPEG) and 3,4-dihydroxymandelic acid ([3H]DOMA) fractions were higher in tissues from ovariectomized rats than during estrus. Ovariectomy also increased significantly the spontaneous efflux of [3H]DOPEG and [3H]DOMA. The metabolic pattern of [3H]noradrenaline both in the tissue and in the efflux from ovariectomized rats was changed by estrogen treatment and became similar to those obtained during estrus. These data suggest a possible modulation of the noradrenaline metabolism by estrogens on the isolated rat uterus.

Download Full-text

Prostaglandins alter the 3H-noradrenaline metabolism by the isolated rat uterus from ovariectiomized animals

Prostaglandins Leukotrienes and Medicine ◽

10.1016/s0262-1746(82)80021-8 ◽

1982 ◽

Vol 9 (3) ◽

pp. 341-348 ◽

Cited By ~ 14

Author(s):

J. Fernández Pardal ◽

M.F. Gimeno ◽

A.L. Gimeno

Keyword(s):

Noradrenaline Metabolism ◽

Rat Uterus ◽

Isolated Rat

Download Full-text

Influence of endogenous and exogenous cyclic 3′ , 5′ -AMP on contractile responses induced by oxytocin and calcium in isolated rat uterus

Life Sciences ◽

10.1016/0024-3205(70)90356-5 ◽

1970 ◽

Vol 9 (3) ◽

pp. 121-128 ◽

Cited By ~ 39

Author(s):

P. Mitznegg ◽

F. Heim ◽

B. Meythaler

Keyword(s):

Rat Uterus ◽

Contractile Responses ◽

Isolated Rat

Download Full-text

Effect of estrogen treatment on plasma oxytocin and vasopressin in ovariectomized rats.

Endocrinologia Japonica ◽

10.1507/endocrj1954.26.197 ◽

1979 ◽

Vol 26 (2) ◽

pp. 197-205 ◽

Cited By ~ 73

Author(s):

KEN'ICHI YAMAGUCHI ◽

TAKAO AKAISHI ◽

HIDEO NEGORO

Keyword(s):

Estrogen Treatment ◽

Ovariectomized Rats

Download Full-text

Stability of A+U-Rich Element Binding Factor 1 (AUF1)-Binding Messenger Ribonucleic Acid Correlates with the Subcellular Relocalization of AUF1 in the Rat Uterus upon Estrogen Treatment

Molecular Endocrinology ◽

10.1210/me.2004-0103 ◽

2004 ◽

Vol 18 (9) ◽

pp. 2255-2267 ◽

Cited By ~ 15

Author(s):

Yukitomo Arao ◽

Atsumi Kikuchi ◽

Mitsuyo Kishida ◽

Masami Yonekura ◽

Akira Inoue ◽

...

Keyword(s):

Ribonucleic Acid ◽

Estrogen Treatment ◽

Rat Uterus ◽

Messenger Ribonucleic Acid ◽

Binding Factor

Download Full-text

Endocrine pharmacology of reproduction (Report 40): Human chorionic gonadotropin receptor in the isolated rat uterus

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)58978-8 ◽

1986 ◽

Vol 40 ◽

pp. 75

Author(s):

Yoshiko Masubuchi ◽

Taiichiro Ohno ◽

Toshio Kumai ◽

Masami Tanaka ◽

Mitsuko Suetsuna ◽

...

Keyword(s):

Chorionic Gonadotropin ◽

Human Chorionic Gonadotropin ◽

Rat Uterus ◽

Gonadotropin Receptor ◽

Isolated Rat

Download Full-text

Estrogen modulates the recruitment of myelopoietic cell progenitors in rat through a stromal cell-independent mechanism involving apoptosis

Blood ◽

10.1182/blood.v87.7.2683.bloodjournal8772683 ◽

1996 ◽

Vol 87 (7) ◽

pp. 2683-2692 ◽

Cited By ~ 33

Author(s):

NK Shevde ◽

JW Pike

Keyword(s):

Estrogen Receptor ◽

Ovarian Function ◽

Morphological Changes ◽

Estrogen Treatment ◽

Ovariectomized Rats ◽

Terminal Deoxynucleotidyl Transferase ◽

Protective Effects ◽

Hematopoietic Stem

Loss of ovarian function leads to a significant increase in the number of bone-resorbing osteoclasts. Estrogen replacement is known to manifest bone protective effects in the treatment of postmenopausal osteoporosis. In the present study, we used ovariectomized rats to examine the effects of estrogen loss at the osteoclast progenitor colony forming unit-granulocyte macrophage (CFU-GM) level. A significant increase in CFU-GM number was observed as early as 7 days following ovariectomy, and correlated directly with an increase in the number of osteoclast-like cells generated in marrow cultures. The increase in CFU-GM following ovariectomy was abrogated in animals that received estrogen treatment in vivo. A similar suppressive effect was observed on CFU-GM number when ovariectomized rat marrow was treated with estrogen in vitro. This effect was blocked in the presence of the estrogen antihormone ICI 164,384. Thus, the data suggest the possibility that estrogen exerts a direct effect on osteoclast progenitors, and does so through the estrogen receptor-mediated mechanism. Ovariectomy also led to an increase in the early hematopoietic stem/progenitor cell population (Thy 1.1+ cells) as determined by FLOW cytometry methods. Morphological changes as well as terminal deoxynucleotidyl transferase assays revealed that estrogen treatment negated growth factor-induced proliferation of these early progenitors by promoting apoptosis. The cellular effects of estrogen in vitro together with the immunocytochemical detection of the estrogen receptor in these cells, strongly support the contention that in addition to osteoclast progenitors such as CFU-GM, earlier hematopoietic progenitors are also unique cellular targets for estrogen action.

Download Full-text