In vitro culture and multiplication of Cryptobia catostomi and experimental infection of white sucker (Catostomus commersoni)

1992 ◽  
Vol 70 (2) ◽  
pp. 201-204 ◽  
Author(s):  
Philip T. Thomas ◽  
Patrick T. K. Woo
Keyword(s):  
Experimental Infection ◽  
Infected Fish ◽  
Nutritional Requirements ◽  
White Sucker ◽  
Catostomus Commersoni ◽  
Minimum Essential Medium ◽  
Large Numbers ◽  
Division Process ◽  
Foetal Bovine Serum

Cryptobia catostomi, a parasitic haemoflagellate of the white sucker (Catostomus commersoni), was cultured in minimum essential medium (MEM) supplemented with Hanks' salts, L-glutamine, and 25% foetal bovine serum (MEM-plus). Parasite numbers were significantly higher in MEM-plus cultures supplemented with white sucker plasma than in unsupplemented cultures. This procedure is useful when large numbers of the parasite are required, e.g., for studies on their nutritional requirements, metabolism, or antigenic nature. Cultures could not be maintained at 10 °C beyond the fourth subculture; this was about 11 months after the primary culture was started. The division process in culture was similar to that reported in fish. The culture forms were infective to white suckers. Parasitaemias in white suckers infected with blood forms increased from 2 to 5 weeks postinfection and stayed relatively constant thereafter. Neither anorexia nor anaemia was evident in infected fish, confirming the nonpathogenicity of C. catostomi to white suckers.

scholarly journals Purification of erythroblastic nests

Blood ◽  
1981 ◽  
Vol 57 (5) ◽  
pp. 922-927
Author(s):  
AJ Macario ◽  
C Dugan ◽  
IL Perez-Lloret ◽  
E Conway de Macario
Keyword(s):  
Spleen Cell ◽  
Trypan Blue ◽  
Single Cells ◽  
Cell Suspensions ◽  
Differential Centrifugation ◽  
Minimum Essential Medium ◽  
Trypan Blue Exclusion ◽  
Large Numbers ◽  
Very High

We describe a method for preparing purified erythroblastic nests in large numbers (approximately 10(6)/run) in three steps: (1) induction of splenic erythropoiesis in mice, (2) preparative differential centrifugation for the removal of erythrocytes and single cells from spleen cell suspensions, and (3) sedimentation in an isokinetic gradient of Ficoll 400 in Joklik's modification of minimum essential medium. Viability of isolated EN is very high, as demonstrated by the trypan blue exclusion and in vitro erythrocyte formation methods.

scholarly journals Purification of erythroblastic nests

Blood ◽  
1981 ◽  
Vol 57 (5) ◽  
pp. 922-927 ◽  
Author(s):  
AJ Macario ◽  
C Dugan ◽  
IL Perez-Lloret ◽  
E Conway de Macario
Keyword(s):  
Spleen Cell ◽  
Trypan Blue ◽  
Single Cells ◽  
Cell Suspensions ◽  
Differential Centrifugation ◽  
Minimum Essential Medium ◽  
Trypan Blue Exclusion ◽  
Large Numbers ◽  
Very High

Abstract We describe a method for preparing purified erythroblastic nests in large numbers (approximately 10(6)/run) in three steps: (1) induction of splenic erythropoiesis in mice, (2) preparative differential centrifugation for the removal of erythrocytes and single cells from spleen cell suspensions, and (3) sedimentation in an isokinetic gradient of Ficoll 400 in Joklik's modification of minimum essential medium. Viability of isolated EN is very high, as demonstrated by the trypan blue exclusion and in vitro erythrocyte formation methods.

Observations on the growth of Eimeria tenella in cultured cells from the parasitized chorioallantoic membranes of the developing chick embryo

Parasitology ◽  
1969 ◽  
Vol 59 (4) ◽  
pp. 757-765 ◽  
Author(s):  
P. L. Long
Keyword(s):  
Technical Assistance ◽  
Cultured Cells ◽  
Simple Procedure ◽  
Eimeria Tenella ◽  
Nutritional Requirements ◽  
Chick Embryos ◽  
Large Numbers ◽  
Different Types

Eimeria tenella infections were established in the chorioallantoic membranes CAM) of developing chick embryos. At different times after infection the CAM ells were cultured in vitro in modified 199 medium. Different types of schizont were grown, some similar to those occurring in infections of the usual in vivo site and others markedly different. In schizogony of one type the merozoites appeared to develop by growing out from the periphery of cellular masses; a process similar to that described by Hammond et al. (1966) for E. bovis.Gametocytes and infective oocysts were also grown by the tissue culture of CAM cells but only in cells infected 4–6 days previously in embryo; CAM cells infected for only 3 days before culture supported the growth of large numbers of schizonts of different types.The majority of the stages grown developed within densely populated areas of epithelial-like cells. A temperature of 41 °C was found to be necessary for the growth of the parasite. The nutritional requirements for the growth of E. tenella are fairly well provided by the medium used. The relatively simple procedure described should be of value for the observation of E. tenella at various stages of its growth and provide a means of in vitro testing of antiparasitic substances.I wish to thank Dr P. P. Levine for his interest and encouragement during the course of the work and Mr D. L'Amoreaux for technical assistance.

The Eosinophilic Cell of the White Sucker, Catostomus commersoni

1976 ◽  
Vol 33 (1) ◽  
pp. 139-144 ◽  
Author(s):  
R. J. G. Lester ◽  
B. A. Daniels
Keyword(s):  
Inflammatory Reaction ◽  
White Sucker ◽  
Catostomus Commersoni ◽  
Large Numbers ◽  
Eosinophilic Cell ◽  
Granular Cytoplasm ◽  
Eosinophilic Granulocytes ◽  
Reticular Cells ◽  
Spherical Nuclei

The inflammatory reaction of Catostomus commersoni to the parasites Actinobdella inequiannulata and Lernea cyprinacea, and to an experimental incision, produced tissue that contained large numbers of eosinophilic granulocytes. The cells had spherical nuclei and orange granular cytoplasm after H & E staining. In imprints of the same tissue treated with Romanowsky stains the cells had a flocculent weakly basophilic cytoplasm and sometimes contained purple granules. They were peroxidase negative. "Finely reticular cells" found in the blood had an identical appearance to the cells observed in the imprints, and were thought to be the same type of cell. They may be equivalent to mammalian eosinophils.

Cytoplasmic Granules in Lymphocytes from Rats Dosed with SK&F 14336-D

1971 ◽  
Vol 29 ◽  
pp. 556-557
Author(s):  
T. G. Merrill ◽  
B. J. Payne ◽  
A. J. Tousimis
Keyword(s):  
Lipid Storage ◽  
Pokeweed Mitogen ◽  
Electron Microscopic ◽  
Cytoplasmic Granules ◽  
Storage Diseases ◽  
Tay Sachs Disease ◽  
Large Numbers ◽  
Microscopic Studies ◽  
Neurovisceral Lipidosis

Rats given SK&F 14336-D (9-[3-Dimethylamino propyl]-2-chloroacridane), a tranquilizing drug, developed an increased number of vacuolated lymphocytes as observed by light microscopy. Vacuoles in peripheral blood of rats and humans apparently are rare and are not usually reported in differential counts. Transforming agents such as phytohemagglutinin and pokeweed mitogen induce similar vacuoles in in vitro cultures of lymphocytes. These vacuoles have also been reported in some of the lipid-storage diseases of humans such as amaurotic familial idiocy, familial neurovisceral lipidosis, lipomucopolysaccharidosis and sphingomyelinosis. Electron microscopic studies of Tay-Sachs' disease and of chloroquine treated swine have demonstrated large numbers of “membranous cytoplasmic granules” in the cytoplasm of neurons, in addition to lymphocytes. The present study was undertaken with the purpose of characterizing the membranous inclusions and developing an experimental animal model which may be used for the study of lipid storage diseases.

Responses in a Fathead Minnow (Pimephales promelas) Lifecycle Test and in Wild White Sucker (Catostomus commersoni) Exposed to a Canadian Bleached Kraft Mill Effluent

2010 ◽  
Vol 45 (2) ◽  
pp. 187-200 ◽  
Author(s):  
Joanne L. Parrott ◽  
L. Mark Hewitt ◽  
Tibor G. Kovacs ◽  
Deborah L. MacLatchy ◽  
Pierre H. Martel ◽  
...  
Keyword(s):  
Egg Production ◽  
Fathead Minnow ◽  
Pimephales Promelas ◽  
Fish Reproduction ◽  
White Sucker ◽  
Catostomus Commersoni ◽  
Liver Size ◽  
Paper Mill Effluent ◽  
Kraft Mill Effluent ◽  
Kraft Mill

Abstract To evaluate currently available bioassays for their use in investigating the causes of pulp and paper mill effluent effects on fish reproduction, the responses of wild white sucker (Catostomus commersoni) collected from the receiving environment at the bleached kraft mill at La Tuque, Quebec, were compared with responses of fathead minnow (Pimephales promelas) exposed to effluent in a laboratory lifecycle test. White sucker collected at effluent exposed sites had increased liver size but none of the reproductive effects that had been documented in earlier field studies at this site. Exposure to 1, 3, 10, 30, and 100% bleached kraft mill effluent (BKME) in the lab led to significantly decreased length, but increased weight and liver size in male fathead minnow. Female length was also decreased and liver size was increased at high effluent exposures. Most effluent concentrations (1 to 30%) significantly increased egg production compared with controls. The fathead minnow lifecycle assay mirrored the effects seen in wild fish captured downstream of the BKME discharge. These results will be used to select short-term fish tests for investigating the causes of and solutions to the effects of mill effluents on fish reproduction.

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