REAL-TIME MONITORING OF MITOCHONDRIAL FUNCTION AND CEREBRAL BLOOD FLOW FOLLOWING FOCAL ISCHEMIA IN RATS

Focal ischemia due to reduction of cerebral blood flow (CBF), creates 2 zones of damage: the core area, which suffers severe damage, and penumbra area, which surrounds the core and suffers intermediate levels of injury. Objectives: A novel method is introduced, which evaluates mitochondrial function in the core and in the penumbra, during focal cerebral ischemia. Methods: Wistar rats underwent focal cerebral ischemia by middle cerebral artery occlusion (MCAO) for 60 minutes, followed by 60 minutes of reperfusion. Mitochondrial function was assessed by a unique Multi-Site – Multi-Parametric (MSMP) monitoring system, which measures mitochondrial NADH using fluorometric technique, and CBF using Laser Doppler Flowmetry (LDF). Results: At the onset of occlusion, CBF dropped and NADH increased significantly only in the right hemisphere. CBF levels were significantly lower and NADH significantly higher in the core than in the penumbra. After reperfusion, CBF and NADH recovered correspondingly to the intensity of ischemia. Conclusion: Application of the MSMP system can add significant information for the understanding of the cerebral metabolic state under ischemic conditions, with an emphasis on mitochondrial function.

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Real-time monitoring of cerebral blood flow by laser speckle contrast imaging after cardiac arrest in rat

2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) ◽

10.1109/embc.2015.7319996 ◽

2015 ◽

Cited By ~ 2

Author(s):

Junyun He ◽

Hongyang Lu ◽

Ruoxian Deng ◽

Leanne Young ◽

Shanbao Tong ◽

...

Keyword(s):

Blood Flow ◽

Cardiac Arrest ◽

Cerebral Blood Flow ◽

Real Time ◽

Laser Speckle ◽

Real Time Monitoring ◽

Contrast Imaging ◽

Laser Speckle Contrast Imaging ◽

Speckle Contrast

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1618: Real-Time Monitoring of Nitric Oxide and Blood Flow During Ischemia-Reperfusion in the Rat Testis

The Journal of Urology ◽

10.1016/s0022-5347(18)33810-2 ◽

2006 ◽

Vol 175 (4S) ◽

pp. 521-521

Author(s):

Motoaki Saito ◽

Tomoharu Kono ◽

Yukako Kinoshita ◽

Itaru Satoh ◽

Keisuke Satoh

Keyword(s):

Nitric Oxide ◽

Blood Flow ◽

Real Time ◽

Ischemia Reperfusion ◽

Real Time Monitoring ◽

Rat Testis

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A Study on the Effect of Neurogenesis and Regulation of GSK3β/PP2A Expression in Acupuncture Treatment of Neural Functional Damage Caused by Focal Ischemia in MCAO Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/962343 ◽

2014 ◽

Vol 2014 ◽

pp. 1-12 ◽

Cited By ~ 8

Author(s):

Ding Luo ◽

Xiaonong Fan ◽

Congcong Ma ◽

Tongtao Fan ◽

Xiaoguang Wang ◽

...

Keyword(s):

Blood Flow ◽

Cerebral Blood Flow ◽

Gene Transcription ◽

Acupuncture Treatment ◽

Sharp Decrease ◽

Underlying Mechanism ◽

Focal Ischemia ◽

Acupuncture Group ◽

Self Healing ◽

Model Group

170 SD rats were randomly divided to five groups. Rats in model group, no-acupuncture group, and acupuncture group were subjected to MCAO surgery. Acupuncture group received 3 consecutive acupuncture treatments at a parameter that deep in 2 mm towards apex nasi and thrust/lifted at 3 times per second for 1 minute, while model group and no-acupuncture group were no-intervention control groups. Serious neural functional damage and sharp decrease of cerebral blood flow, obvious infarction volume, increased nestin mRNA expression, and immunopositive cells population (nestin+, BrdU+and nestin/BrdU+) were found in MCAO rats which had not been observed in normal group and sham-operated group. However, the damage was attenuated by rat’s “self-healing” capacity 3 days after MCAO. And the “self-healing” capacity can be strengthen by acupuncture treatment through increasing cerebral blood flow, neurogenesis, and regulation of gene transcription or GSK-3βand PP2A expression. In conclusion, the present study indicates that the underlying mechanism of acupuncture treatment on neural functional damage caused by focal ischemia injury is a multiple interaction which may involve improved cerebral blood supply, neurogenesis, and regulation of gene transcription or GSK-3βand PP2A expression in MCAO rats.

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Late Reversal of Cerebral Perfusion and Water Diffusion after Transient Focal Ischemia in Rats

Journal of Cerebral Blood Flow & Metabolism ◽

10.1097/00004647-200203000-00002 ◽

2002 ◽

Vol 22 (3) ◽

pp. 253-261 ◽

Cited By ~ 22

Author(s):

Lei Wang ◽

Victor E. Yushmanov ◽

Serguei M. Liachenko ◽

Pei Tang ◽

Ronald L. Hamilton ◽

...

Keyword(s):

Blood Flow ◽

Cerebral Blood Flow ◽

Focal Ischemia ◽

Artery Occlusion ◽

Tissue Water ◽

Apparent Diffusion ◽

Reperfusion Period ◽

Adc Values ◽

Transient Focal Ischemia ◽

Late Reperfusion

Region-specific cerebral blood flow (CBF) and the apparent diffusion coefficient (ADC) of tissue water in the rat brain were quantified by high-field magnetic resonance imaging at 9.4 T in the rat suture occlusion model. Cerebral blood flow and ADC were compared during the short- (4.5 hours) and long-term (up to 6 days) reperfusion after 80 minutes of transient middle cerebral artery occlusion, and correlated with the histology analysis. On occlusion, average CBF fell from ∼100 to less than 50 mL 100 g−1 min−1 in the cortex, and to less than 20 mL 100 g−1 min−1 in the caudate putamen (CP). Corresponding ADC values decreased from (6.98 ± 0.82) × 10−4 to (5.49 ± 0.54) × 10−4 mm2/s in the cortex, and from (7.16 ± 0.58) × 10−4 to (4.86 ± 0.62) × 10−4 mm2/s in the CP. On average, CBF recovered to ∼50% of baseline in the first 24 hours of reperfusion. After 2 to 4 days, a strong hyperperfusion in the ipsilateral cortex and CP, up to ∼300 mL 100 g−1 min−1, was observed. The ADC ratio in the ipsilateral and contralateral CP was also inverted in the late reperfusion period. Histology revealed more severe tissue damage at the late stage of reperfusion than at 4.5 hours. Significant reversal of CBF and ADC during the late reperfusion period may reflect the impairment of autoregulation in the ischemic regions. Vascular factors may play an important role in the infarct development after 80-minute focal ischemia.

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