Regulated functions | ScienceGate

We isolated the matrix attachment region-binding protein (MBP) DMBP-1 fromDunaliella salinain our previous studies. MBPs are part of the cis-acting protein family cluster. The regulatory function possibly works through the interaction of the MBPs with each other. In the present study, DMBP-1 was used as the bait in screening theD. salinacDNA library for DMBP-1 interactors that could potentially mediate the DMBP-1-regulated functions. A novel MBP, namely, DMBP-2, was identified as a DMBP-1 binding partner. The cDNA of DMBP-1 was 823 bp long and contained a 573 bp open reading frame, which encoded a polypeptide of 191 amino acids. The interaction between DMBP-2 and DMBP-1 was further confirmed through glutathione S-transferase pull-down assays.

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Breast cancer and sphingolipid signalling

Journal of Dairy Research ◽

10.1017/s0022029905001238 ◽

2005 ◽

Vol 72 (S1) ◽

pp. 5-13 ◽

Cited By ~ 2

Author(s):

Paola Signorelli ◽

Riccardo Ghidoni

Keyword(s):

Breast Cancer ◽

Cancer Prevention ◽

Biological Membranes ◽

Breast Cancer Prevention ◽

Intracellular Signalling ◽

Regulated Functions ◽

Tumour Initiation ◽

Prevention And Therapy

Cancer develops from overlapping events that tend to deregulate the metabolism and unbalance the homeostasis of cells. Sphingolipids, major components of biological membranes, are also mediators of intracellular signalling. Their metabolism can be influenced by diverse stimuli and the accumulation or deficiency of intermediates may trigger proliferation and/or impair the ability of damaged cells to undergo apoptosis. Many sphingolipid-regulated functions are implicated in tumour initiation, promotion, progression and responsiveness to chemotherapy. In this review, evidence of the alteration of sphingolipids metabolism and signalling will be discussed in breast cancer prevention and therapy.

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Distinctly regulated functions and mobilization of CD11c-positive cells elicited by TLR3- and IPS-1 signaling in the cornea

Immunology Letters ◽

10.1016/j.imlet.2018.12.004 ◽

2019 ◽

Vol 206 ◽

pp. 49-53 ◽

Cited By ~ 1

Author(s):

Mayumi Ueta ◽

Junji Hamuro ◽

Suzuko Ohsako ◽

Shigeru Kinoshita

Keyword(s):

Regulated Functions

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Deficient cyclic adenosine 3',5'-monophosphate control in mutants of two genes of Neurospora crassa.

Molecular and Cellular Biology ◽

10.1128/mcb.1.1.1 ◽

1981 ◽

Vol 1 (1) ◽

pp. 1-8 ◽

Cited By ~ 13

Author(s):

M L Pall ◽

J M Trevillyan ◽

N Hinman

Keyword(s):

Plasma Membrane ◽

Membrane Potential ◽

Cyclic Amp ◽

Adenylate Cyclase ◽

Neurospora Crassa ◽

Alternative Oxidase ◽

Oscillatory Behavior ◽

Wild Type ◽

Glucose Transport System ◽

Regulated Functions

Strains of Neurospora crassa mutant in either of two genes, Crisp-1 (cr1) and Frost (fr), showed no increase of cyclic adenosine 3',5'-monophosphate (cyclic AMP) levels when subjected to several treatments which produce large increases of cyclic AMP in wild-type Neurospora. Evidently, the previously reported deficiencies of adenylate cyclase in these mutants were sufficient to block the normal increases. This fact suggests that both mutants could be used to help determine which control phenomena involve cyclic AMP and to interrupt the control of established cyclic AMP-regulated functions. Earlier studies had suggested an interdependence of the cyclic AMP level and the electric potential difference across the plasma membrane of Neurospora. Present experiments, therefore, employed several strains with the cr1 mutation to test for possible roles of cyclic AMP in recovery and oscillatory behavior of the Neurospora membrane potential. The results showed all such phenomena to be normal in the adenylate cyclase-defective strains, which demonstrates that variations of cyclic AMP are not obligatorily involved in the apparent control processes. Evidence is also presented that the induction of both glucose transport system II and the alternative oxidase do not require elevated cyclic AMP levels.

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