Wen-Jing-Tang, a Traditional Chinese Herbal Medicine Increases Luteinizing Hormone Release In Vitro

1986 ◽  
Vol 14 (03n04) ◽  
pp. 157-160 ◽  
Author(s):  
Akira Miyake ◽  
Jin-Woo Lee ◽  
Keiichi Tasaka ◽  
Shirou Ohtsuka ◽  
Toshihiro Aono
Keyword(s):  
Herbal Medicine ◽  
Luteinizing Hormone ◽  
Chinese Herbal Medicine ◽  
Female Rats ◽  
Normal Female ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Lh Release ◽  
Lh Rh

For examination of the effect on luteinizing hormone (LH) release of Wen-Jing-Tang, a traditional Chinese herbal medicine, the pituitary from normal female rats in diestrus was perifused alone or in sequence with the mediobasal hypothalamus (MBH) in a sequential double-chamber perifusion system. Wen-Jing-Tang at 5 or 500 μg/ml induced significant LH release (60-95 % increase) from the pituitary in series with the MBH, but had no effect on LH release from the pituitary perifused alone. These data suggest that Wen-Jing-Tang induces LH release from the pituitary through hypothalamic LH-RH.

Effect of a Traditional Chinese Herbal Medicine (San Zhuang Wan) on the Hypothalamus-Pituitary-Testis Axis of the Immature Rat

1989 ◽  
Vol 17 (03n04) ◽  
pp. 171-175
Author(s):  
Ting-Xin Zhang ◽  
David K. Pomerantz
Keyword(s):  
Herbal Medicine ◽  
Cyclic Amp ◽  
Chinese Herbal Medicine ◽  
Testicular Tissue ◽  
Male Rat ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Male Impotence ◽  
Testicular Weight

San Zhuang Wan (SZW) is a traditional Chinese herbal medicine used to treat male impotence. The mechanism of its therapeutic effect is not known nor have any of its potential physiologic actions been investigated. We examined the effect of SZW adminstered by gavage, on the reproductive system of the prepubertal male rat. After 10 days treatment of 21-day-old rats the concentration of testosterone in serum and testicular weight and decrease whereas the serum concentration of luteinizing hormone (LH) had significantly increased. When administered to gonadectomized rats SZW partially restored the weight of the accessory sex organs and concentration of LH toward normal, but serum androgen concentration was not changed. In vitro assessment of fragments of testicular tissue or purified Leydig cells for responsiveness to maximal doses of LH or cyclic AMP showed that SWZ significantly reduced testosterone secretion in response to either stimulant. The data demonstrate that SWZ has potent effects on the reproductive axis of the pubertal male rat. At least one locus for such action is on the Leydig cell at a point distal to the formation of cyclic AMP. Further study is necessary to determine whether these effects could be involved with or independent of the mechanism of the therapeutic action of SZW.

scholarly journals TheIn VitroandIn VivoWound Healing Properties of the Chinese Herbal Medicine “Jinchuang Ointment”

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Tsung-Jung Ho ◽  
Shinn-Jong Jiang ◽  
Guang-Huey Lin ◽  
Tzong Shiun Li ◽  
Lih-Ming Yiin ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Scientific Basis ◽  
Tube Formation ◽  
University Hospital ◽  
Chemical Compositions ◽  
Dragon’S Blood ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal

“Jinchuang ointment” is a traditional Chinese herbal medicine complex for treatment of incised wounds. For more than ten years, it has been used at China Medical University Hospital (Taichung, Taiwan) for the treatment of diabetic foot infections and decubitus ulcers. Three different cases are presented in this study. “Jinchuang” ointment is a mixture of natural product complexes from nine different components, making it difficult to analyze its exact chemical compositions. To further characterize the herbal ingredients used in this study, the contents of reference standards present in a subset of the ointment ingredients (dragon’s blood, catechu, frankincense, and myrrh) were determined by HPLC. Twoin vitrocell based assay platforms, wound healing and tube formation, were used to examine the biological activity of this medicine. Our results show that this herbal medicine possesses strong activities including stimulation of angiogenesis, cell proliferation, and cell migration, which provide the scientific basis for its clinically observed curative effects on nonhealing diabetic wounds.

Clomiphene citrate induces luteinizing hormone release through hypothalamic luteinizing hormone-releasing hormone in vitro

1983 ◽  
Vol 103 (3) ◽  
pp. 289-292 ◽  
Author(s):  
A. Miyake ◽  
K. Tasaka ◽  
T. Sakumoto ◽  
Y. Kawamura ◽  
Y. Nagahara ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Clomiphene Citrate ◽  
Female Rats ◽  
Normal Female ◽  
Releasing Hormone ◽  
Superfusion System ◽  
In Series ◽  
Lh Release ◽  
Lh Releasing Hormone

Abstract. The releasing effects of clomiphene citrate (clomiphene) on luteinizing hormone (LH) and LH-releasing hormone (LRH) were examined in a sequential double chamber superfusion system by superfusing the mediobasal hypothalami (MBH) and/or pituitaries excised from normal female rats in dioestrus. When the MBH and the pituitary were superfused in sequence with medium containing 2 × 10−10 m oestradiol (E2), two significant peaks in LH release (60–130% increase, P < 0.05) were observed 40 min and 90 min after the administration of 3 × 10−8 mol clomiphene. Administration of clomiphene in medium without E2 induced a low peak (25–50% increase, P < 0.05) of LH released from the pituitary perfused in series with the MBH. Administration of clomiphene did not cause a marked increase of LH from the pituitary superfused alone, when superfused with or without E2 containing medium. The concentration of LRH in the efflux was significantly increased (50–100%) 40 min and 90 min after clomiphene administration when MBH was superfused with medium containing E2, whereas clomiphene had no effect when superfused with medium alone. These data indicate: 1) that clomiphene induces LRH release from the MBH, that it may induce LH release, in part, by acting directly at the pituitary level; 2) that changes in LH after clomiphene administration coincide with LRH release, and 3) that a certain concentration of E2 may be necessary for the secretion of LRH by clomiphene.

Hydrocortisone elicits the effect of clomiphene citrate on luteinizing hormone-releasing hormone release in vitro

1984 ◽  
Vol 107 (2) ◽  
pp. 145-148 ◽  
Author(s):  
Akira Miyake ◽  
Keiichi Tasaka ◽  
Tetsuro Sakumoto ◽  
Yasuhito Nagahara ◽  
Toshihiro Aono
Keyword(s):  
Luteinizing Hormone ◽  
Clomiphene Citrate ◽  
Female Rats ◽  
Normal Female ◽  
Releasing Hormone ◽  
In Series ◽  
Significant Release ◽  
Lh Release ◽  
Lh Releasing Hormone

Abstract. The effect of hydrocortisone on the release of luteinizing hormone (LH) and LH-releasing hormone (LRH) in response to clomiphene citrate (clomiphene) were examined in a sequential double chamber perifusion system by perifusing the mediobasal hypothalami (MBH) and/or pituitaries excised from normal female rats in dioestrus. When the MBH and the pituitary were perifused in sequence with medium containing 5 × 10−6 m hydrocortisone, a significant release in LH (100– 150% increase, P < 0.01–P < 0.05) was observed 40 min after the administration of 3 × 10−8 mol clomiphene. Clomiphene had no effect on LH release from the pituitary when perifused in series with the MBH without basal hydrocortisone infusion. Administration of clomiphene did not cause a significant increase in LH from the pituitary perifused alone, with or without medium containing hydrocortisone. The concentration of LRH in the efflux was significantly increased 40 min after clomiphene administration when MBH was perifused with medium containing hydrocortisone, whereas clomiphene had no effect when perifused with medium only. These data indicate that hydrocortisone stimulates the effect of clomiphene on LRH release from the hypothalamus, which in turn induces LH release from the pituitary.

Study of the ESI and APCI interfaces for the UPLC–MS/MS analysis of pesticides in traditional Chinese herbal medicine

2013 ◽  
Vol 406 (5) ◽  
pp. 1481-1491 ◽  
Author(s):  
Lina Chen ◽  
Fengrui Song ◽  
Zhiqiang Liu ◽  
Zhong Zheng ◽  
Junpeng Xing ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Traditional Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Ms Analysis
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