Transposon Tn5

2008 ◽  
Vol 42 (1) ◽  
pp. 269-286 ◽  
Author(s):  
William S. Reznikoff
Keyword(s):  
Transposon Tn5

Chromosome mobilization of Legionella pneumophila with RK2::Mu and Tn5-Mob

1992 ◽  
Vol 38 (7) ◽  
pp. 664-671 ◽  
Author(s):  
Clifford S. Mintz ◽  
Chang Hua Zou
Keyword(s):  
Genetic Mapping ◽  
Key Words ◽  
Legionella Pneumophila ◽  
Transposon Tn5 ◽  
Helper Plasmid ◽  
Chromosome Transfer ◽  
Chromosomal Markers

RK2::Mu plasmids and transposon Tn5-Mob were used to mobilize the Legionella pneumophila chromosome. Plate matings between L. pneumophila donors that contained RK2::Mu plasmids and auxotrophic recipients yielded recombinants at fequencies ranging from 10−6 to 10−7 per recipient for the markers tested. The presence of a Mu insertion in the chromosome of donors that harbored RK2::Mu plasmids increased the frequency of chromosome transfer of certain selected markers as compared with strains that contained RK2::Mu alone. Cotransfer experiments with Mu-containing donors and a thymidine and tryptophan auxotroph failed to reveal any linkage between the thy and trp loci in L. pneumophila. A strain that contained a chromosomal Tn5-Mob insertion and helper plasmid pRK24.4 transferred chromosomal markers at frequencies of 10−7 per recipient. These findings suggest that RK2::Mu plasmids and Tn5-Mob may be useful for genetic mapping experiments with L. pneumophila. Key words: Legionella pneumophila, chromosome transfer, Tn5-Mob, RK2::Mu.

Localized transposon Tn5 mutagenesis of the photosynthetic gene cluster of Rhodobacter sphaeroides

1990 ◽  
Vol 4 (6) ◽  
pp. 977-989 ◽  
Author(s):  
S. A. Coomber ◽  
M. Chaudhri ◽  
A. Connor ◽  
G. Britton ◽  
C. N. Hunter
Keyword(s):  
Gene Cluster ◽  
Rhodobacter Sphaeroides ◽  
Transposon Tn5 ◽  
Tn5 Mutagenesis ◽  
Photosynthetic Gene ◽  
Transposon Tn5 Mutagenesis
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