scholarly journals Tn5tac1, a derivative of transposon Tn5 that generates conditional mutations.

1988 ◽  
Vol 85 (17) ◽  
pp. 6468-6472 ◽  
Author(s):  
W. Y. Chow ◽  
D. E. Berg
Keyword(s):  
Transposon Tn5 ◽  
Conditional Mutations

scholarly journals Suppressor Analysis of the Saccharomyces cerevisiae Gene REC104 Reveals a Genetic Interaction With REC102

Genetics ◽  
1999 ◽  
Vol 151 (4) ◽  
pp. 1261-1272 ◽  
Author(s):  
Laura Salem ◽  
Natalie Walter ◽  
Robert Malone
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Null Allele ◽  
Genetic Interaction ◽  
In Vitro Mutagenesis ◽  
Conditional Mutations ◽  
Suppressor Analysis

Abstract REC104 is a gene required for the initiation of meiotic recombination in Saccharomyces cerevisiae. To better understand the role of REC104 in meiosis, we used an in vitro mutagenesis technique to create a set of temperature-conditional mutations in REC104 and used one ts allele (rec104-8) in a screen for highcopy suppressors. An increased dosage of the early exchange gene REC102 was found to suppress the conditional recombinational reduction in rec104-8 as well as in several other conditional rec104 alleles. However, no suppression was observed for a null allele of REC104, indicating that the suppression by REC102 is not “bypass” suppression. Overexpression of the early meiotic genes REC114, RAD50, HOP1, and RED1 fails to suppress any of the rec104 conditional alleles, indicating that the suppression might be specific to REC102.

Chromosome mobilization of Legionella pneumophila with RK2::Mu and Tn5-Mob

1992 ◽  
Vol 38 (7) ◽  
pp. 664-671 ◽  
Author(s):  
Clifford S. Mintz ◽  
Chang Hua Zou
Keyword(s):  
Genetic Mapping ◽  
Key Words ◽  
Legionella Pneumophila ◽  
Transposon Tn5 ◽  
Helper Plasmid ◽  
Chromosome Transfer ◽  
Chromosomal Markers

RK2::Mu plasmids and transposon Tn5-Mob were used to mobilize the Legionella pneumophila chromosome. Plate matings between L. pneumophila donors that contained RK2::Mu plasmids and auxotrophic recipients yielded recombinants at fequencies ranging from 10−6 to 10−7 per recipient for the markers tested. The presence of a Mu insertion in the chromosome of donors that harbored RK2::Mu plasmids increased the frequency of chromosome transfer of certain selected markers as compared with strains that contained RK2::Mu alone. Cotransfer experiments with Mu-containing donors and a thymidine and tryptophan auxotroph failed to reveal any linkage between the thy and trp loci in L. pneumophila. A strain that contained a chromosomal Tn5-Mob insertion and helper plasmid pRK24.4 transferred chromosomal markers at frequencies of 10−7 per recipient. These findings suggest that RK2::Mu plasmids and Tn5-Mob may be useful for genetic mapping experiments with L. pneumophila. Key words: Legionella pneumophila, chromosome transfer, Tn5-Mob, RK2::Mu.

Localized transposon Tn5 mutagenesis of the photosynthetic gene cluster of Rhodobacter sphaeroides

1990 ◽  
Vol 4 (6) ◽  
pp. 977-989 ◽  
Author(s):  
S. A. Coomber ◽  
M. Chaudhri ◽  
A. Connor ◽  
G. Britton ◽  
C. N. Hunter
Keyword(s):  
Gene Cluster ◽  
Rhodobacter Sphaeroides ◽  
Transposon Tn5 ◽  
Tn5 Mutagenesis ◽  
Photosynthetic Gene ◽  
Transposon Tn5 Mutagenesis
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