Corrosion Behavior of Calcium Phosphate-Coated Biomedical Magnesium Alloy Under in Vitro and In Vivo Environments

In this study a calcium phosphate (Ca–P) coating was fabricated on the surface of an AZ31 alloy by a chemical deposition process, and the in vitro and in vivo studies were carried out on a Ca–P-coated and uncoated AZ31 alloy to determine the effect of Ca–P coating on the corrosion behavior and biocompatibility of the AZ31 alloy. The morphology and composition of the Ca–P coating were characterized by scanning electron microscopy and energy dispersive spectroscopy. The corrosion behavior of the Ca-P coating was evaluated by a static immersion test and the effects of the Ca–P coating on biocompatibility were also investigated by in vitro cell experiments and in vivo animal experiments. The results indicated that the Ca–P coating reduced the in vitro and in vivo corrosion rates of the AZ31 alloy. Cell experiments showed significantly good adherence and high proliferation on the Ca–P-coated AZ31 alloy than those on the uncoated AZ31 alloy ( P < 0.05). The blood cell aggregation tests showed that the Ca–P-coated AZ31 alloy had decreased the blood cell aggregation compared to the uncoated AZ31 alloy. The animal experiments showed that the uncoated AZ31 alloy degraded more rapidly than the Ca–P-coated AZ31 alloy and the Ca–P coating provided significantly good biocompatibility, thus suggesting that the Ca–P coating not only slowed down the corrosion rate of the AZ31 alloy, but also improved its biocompatibility. Therefore, the Ca–P-coated AZ31 alloy can be considered as a promising biomaterial for orthopedic applications.

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A Comprehensive Study of Osteogenic Calcium Phosphate Silicate Cement: Material Characterization and In Vitro/In Vivo Testing

Advanced Healthcare Materials ◽

10.1002/adhm.201500469 ◽

2015 ◽

Vol 5 (4) ◽

pp. 457-466 ◽

Cited By ~ 10

Author(s):

Tianxing Gong ◽

Zhiqin Wang ◽

Yixi Zhang ◽

Yubiao Zhang ◽

Mingxiao Hou ◽

...

Keyword(s):

Calcium Phosphate ◽

Material Characterization ◽

Cement Material ◽

Phosphate Silicate ◽

In Vivo Testing ◽

Comprehensive Study

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Calcium Phosphate Coating on Blast-Treated Titanium Implants by RF Magnetron Sputtering

Materials Science Forum ◽

10.4028/www.scientific.net/msf.631-632.211 ◽

2009 ◽

Vol 631-632 ◽

pp. 211-216 ◽

Cited By ~ 3

Author(s):

Kyosuke Ueda ◽

Takayuki Narushima ◽

Takashi Goto ◽

T. Katsube ◽

Hironobu Nakagawa ◽

...

Keyword(s):

Calcium Phosphate ◽

Magnetron Sputtering ◽

Bonding Strength ◽

Rf Magnetron Sputtering ◽

Titanium Implants ◽

Calcium Phosphate Coating ◽

Phosphate Coating ◽

Coating Films

Calcium phosphate coating films were fabricated on Ti-6Al-4V plates and screw-type implants with a blast-treated surface using radiofrequency (RF) magnetron sputtering and were evaluated in vitro and in vivo. Amorphous calcium phosphate (ACP) and oxyapatite (OAp) films obtained in this study could cover the blast-treated substrate very efficiently, maintaining the surface roughness. For the in vitro evaluations of the calcium phosphate coating films, bonding strength and alkaline phosphatase (ALP) activity were examined. The bonding strength of the coating films to a blast-treated substrate exceeded 60 MPa, independent of film phases except for the film after post-heat-treatment in silica ampoule. When compared with an uncoated substrate, the increase in the ALP activity of osteoblastic SaOS-2 cells on a calcium phosphate coated substrate was confirmed by a cell culture test. The removal torque of screw-type Ti-6Al-4V implants with a blast-treated surface from the femur of Japanese white rabbit increased with the duration of implantation and it was statistically improved by coating an ACP film 2 weeks after implantation. The in vitro and in vivo studies suggested that the application of the sputtered ACP film as a coating on titanium implants was effective in improving their biocompatibility with bones.

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In vitro and in vivo biocompatibility and corrosion behaviour of a bioabsorbable magnesium alloy coated with octacalcium phosphate and hydroxyapatite

Acta Biomaterialia ◽

10.1016/j.actbio.2014.09.026 ◽

2015 ◽

Vol 11 ◽

pp. 520-530 ◽

Cited By ~ 104

Author(s):

Sachiko Hiromoto ◽

Motoki Inoue ◽

Tetsushi Taguchi ◽

Misao Yamane ◽

Naofumi Ohtsu

Keyword(s):

Magnesium Alloy ◽

Corrosion Behaviour ◽

Octacalcium Phosphate

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Enhanced effect of β-tricalcium phosphate phase on neovascularization of porous calcium phosphate ceramics: In vitro and in vivo evidence

Acta Biomaterialia ◽

10.1016/j.actbio.2014.09.028 ◽

2015 ◽

Vol 11 ◽

pp. 435-448 ◽

Cited By ~ 60

Author(s):

Y. Chen ◽

J. Wang ◽

X.D. Zhu ◽

Z.R. Tang ◽

X. Yang ◽

...

Keyword(s):

Calcium Phosphate ◽

Tricalcium Phosphate ◽

Calcium Phosphate Ceramics ◽

Phosphate Phase

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Biocompatibility and Bone Formation of Flexible, Cotton Wool-like PLGA/Calcium Phosphate Nanocomposites in Sheep

The Open Orthopaedics Journal ◽

10.2174/1874325001105010063 ◽

2011 ◽

Vol 5 (1) ◽

pp. 63-71 ◽

Cited By ~ 35

Author(s):

Oliver D Schneider ◽

Dirk Mohn ◽

Roland Fuhrer ◽

Karina Klein ◽

Käthi Kämpf ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Formation ◽

Antimicrobial Properties ◽

Drill Hole ◽

Bone Defects ◽

Long Bone ◽

Minimal Amount ◽

Cotton Wool

Background: The purpose of this preliminary study was to assess the in vivo performance of synthetic, cotton wool-like nanocomposites consisting of a biodegradable poly(lactide-co-glycolide) fibrous matrix and containing either calcium phosphate nanoparticles (PLGA/CaP 60:40) or silver doped CaP nanoparticles (PLGA/Ag-CaP 60:40). Besides its extraordinary in vitro bioactivity the latter biomaterial (0.4 wt% total silver concentration) provides additional antimicrobial properties for treating bone defects exposed to microorganisms. Materials and Methods: Both flexible artificial bone substitutes were implanted into totally 16 epiphyseal and metaphyseal drill hole defects of long bone in sheep and followed for 8 weeks. Histological and histomorphological analyses were conducted to evaluate the biocompatibility and bone formation applying a score system. The influence of silver on the in vivo performance was further investigated. Results: Semi-quantitative evaluation of histology sections showed for both implant materials an excellent biocompatibility and bone healing with no resorption in the adjacent bone. No signs of inflammation were detectable, either macroscopically or microscopically, as was evident in 5 µm plastic sections by the minimal amount of inflammatory cells. The fibrous biomaterials enabled bone formation directly in the centre of the former defect. The area fraction of new bone formation as determined histomorphometrically after 8 weeks implantation was very similar with 20.5 ± 11.2 % and 22.5 ± 9.2 % for PLGA/CaP and PLGA/Ag-CaP, respectively. Conclusions: The cotton wool-like bone substitute material is easily applicable, biocompatible and might be beneficial in minimal invasive surgery for treating bone defects.

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