Age-related changes of bradykinin B1 and B2 receptors in rat heart

Aging is a major risk factor for the development of vascular diseases, such as hypertension and atherosclerosis, that leads to end organ damage and especially heart failure. Bradykinin has been demonstrated to have a cardioprotective role by affecting metabolic processes and tissue perfusion under conditions of myocardial ischemia. Its actions are exerted via the bradykinin B1- and B2-type receptors (B1Rs and B2Rs), but the functional status of these receptors during the aging process is poorly understood. This study aims to investigate whether changes in B1R and B2R gene and protein expression in rat heart are associated with the age-related alterations of cardiac structure and function. Using real-time PCR, we found that B1R mRNA expression increased 2.9-fold in hearts of older rats (24 mo of age) compared with younger rats (3 mo of age), whereas B2R gene expression remained unchanged. Western blot analysis showed that expression of B2R at the protein level is approximately twofold higher in young rats compared with old rats, whereas the B1R protein is approximately twofold higher in old rats compared with young rats. The present results provide clear functional and molecular evidence that indicate age-related changes of bradykinin B1Rs and B2Rs in heart. Because the cardioprotective actions of bradykinin are physiologically mediated via the B2Rs, whereas the B1Rs become induced by tissue damage, these results suggest that age-related decreases in B2R protein levels may leave the heart vulnerable to ischemic damage, and increases in B1R expression and activity may represent a compensatory reaction in aging hearts.

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Effect of age on L-3,5,3'-triiodothyronine-induced ethane exhalation

Journal of Applied Physiology ◽

10.1152/jappl.1994.77.1.160 ◽

1994 ◽

Vol 77 (1) ◽

pp. 160-164 ◽

Cited By ~ 8

Author(s):

A. D. Mooradian ◽

M. P. Habib ◽

F. Dickerson ◽

T. Yetskievych

Keyword(s):

Food Restriction ◽

Aged Rats ◽

Basal Rate ◽

Young Rats ◽

Age Related ◽

Old Rats ◽

The Mean ◽

Ad Libitum ◽

Effect Of Age ◽

Age Related Changes

The effect of age on thyroid hormone-induced lipid peroxidation in rats was determined by measuring ethane exhalation (EE) rate. The mean basal EE rates (in pmol.min-1 x 100 g-1) at room air in 25-mo-old aged rats [3.07 +/- 0.26 (SE)] and in intermediate age (18-mo-old) rats (3.42 +/- 0.18) were significantly lower than that in young (3- to 5-mo-old) rats (4.44 +/- 0.27) (P < 0.01). After 8 days of L-3,5,3′-triiodothyronine (T3) administration, EE rates in aged rats (4.10 +/- 0.25) and in intermediate-age rats (4.16 +/- 0.35) were significantly lower than that in young rats (5.61 +/- 0.36) (P < 0.01). T3-treated young rats pair-fed with aged rats for 2 wk had EE rates (5.53 +/- 0.18) comparable to ad libitum-fed young rats, although the basal rate of EE was significantly reduced with partial food restriction (4.44 +/- 0.27 vs. 3.50 +/- 0.24) (P < 0.01). These observations indicate that reduced food intake with age cannot account for the age-related changes in T3-stimulated peroxidation rate of lipids.

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Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage

Immunity ◽

10.1016/j.immuni.2021.04.025 ◽

2021 ◽

Author(s):

Anna Barkaway ◽

Loïc Rolas ◽

Régis Joulia ◽

Jennifer Bodkin ◽

Tchern Lenn ◽

...

Keyword(s):

Organ Damage ◽

Age Related ◽

Remote Organ ◽

Age Related Changes

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Age-related changes in endothelial permeability and distribution volume of albumin in rat aorta

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1993.264.3.h679 ◽

1993 ◽

Vol 264 (3) ◽

pp. H679-H685 ◽

Cited By ~ 7

Author(s):

J. Belmin ◽

B. Corman ◽

R. Merval ◽

A. Tedgui

Keyword(s):

Arterial Wall ◽

Relative Concentration ◽

Endothelial Permeability ◽

Distribution Volume ◽

Albumin Concentration ◽

Macromolecular Transport ◽

Age Related ◽

The Media ◽

Old Rats ◽

Age Related Changes

Age-related changes in macromolecular transport across the arterial wall were investigated in 10-, 20-, and 30-mo-old WAG/Rij rats. Animals were injected with 125I- and 131I-labeled albumin, 90 and 5 min before they were killed, respectively. The transmural distribution of relative concentration of tracers in the aortic wall was obtained using en face serial sectioning technique. The apparent endothelial permeability to albumin calculated from the distribution of 5-min 131I-labeled albumin concentrations was significantly enhanced in 20- and 30-mo-old rats compared with 10-mo-old rats. The apparent distribution volume of albumin within the media, estimated as the mean medial 125I-labeled albumin concentration, was not significantly changed in 20-mo-old rats but was significantly decreased in the 30-mo-old animals. These age-related changes in the macromolecular transport suggest that the entry of plasma macromolecules in the aged arterial wall might be enhanced, whereas the efflux through the media may be impeded, possibly contributing to their trapping in the subendothelium.

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Gut Microbiota Is Involved in Alcohol-Induced Osteoporosis in Young and Old Rats Through Immune Regulation

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2021.636231 ◽

2021 ◽

Vol 11 ◽

Author(s):

Ming Cheng ◽

Bo Tan ◽

Xiaojing Wu ◽

Feng Liao ◽

Fei Wang ◽

...

Keyword(s):

Alcohol Consumption ◽

Gut Microbiota ◽

Bone Metabolism ◽

Alcohol Metabolism ◽

Excessive Alcohol Consumption ◽

Young Rats ◽

Age Related ◽

Different Ages ◽

Old Rats

Long-term and excessive alcohol consumption are risk factors for osteoporosis. Excessive drinking can reduce bone density and also cause imbalance of gut microbiota. And gut microbiota can affect bone metabolism through various mechanisms, and the regulation of gut microbiota is closely related to age. However, the effects of gut microbiota on alcohol-induced osteoporosis at different ages are unclear. In this study, young and old rats were used to induce osteoporosis by long-term alcohol consumption, and alcohol metabolism, bone morphology, bone absorption and immune activity of rats were analyzed to determine the effects of alcohol on rats of different ages. In addition, changes of gut microbiota in rats were analyzed to explore the role of gut microbiota in alcohol-induced osteoporosis in rats of different ages. The results showed the ability of alcohol metabolism was only associated with age, but not with alcohol consumption. Long-term alcohol consumption resulted in the changes of bone metabolism regulating hormones, bone loss, activation of receptor activator of NF-κB ligand (RANKL) signaling and inflammatory response. And osteoporosis was more severe in old rats than young rats, suggesting that alcohol-induced osteoporosis is age-related. In addition, long-term drinking also affected the composition of gut microbiota in rats, with a significant increase in the proportion of pro-inflammatory microorganisms. Overall, this study found that long-term alcohol consumption induced osteoporosis and affected the composition of gut microbiota. And alcohol can activate T lymphocytes directly or indirectly by regulating the changes of gut microbiota to produce cytokines, and further activate osteoclasts. In addition, the osteoporosis was more severe in the old rats than young rats, which may be due to the higher diversity and stronger regulation ability of gut microbiota in young rats compared with old rats.

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Age related changes in the expression and activity of nitric oxide synthase isoforms in the rat heart

Journal of Molecular and Cellular Cardiology ◽

10.1016/s0022-2828(02)90796-0 ◽

2002 ◽

Vol 34 (6) ◽

pp. A18

Author(s):

T. Damy ◽

P. Ratajczak ◽

F. Marotte ◽

J. Boczkowski ◽

E. Robidel ◽

...

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Rat Heart ◽

Age Related ◽

Oxide Synthase ◽

Expression And Activity ◽

Age Related Changes

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Age-related changes in substance P-immunoreactive nerve structures of the rat recto-anal region

Open Medicine ◽

10.2478/s11536-009-0098-2 ◽

2010 ◽

Vol 5 (3) ◽

pp. 358-364

Author(s):

Nikolai Lazarov ◽

Dimitar Itzev ◽

Kamen Usunoff ◽

Negrin Negrev ◽

Radomir Radomirov

Keyword(s):

Substance P ◽

Anal Sphincter ◽

Internal Anal Sphincter ◽

Circular Muscle ◽

Muscle Layer ◽

Nerve Fibres ◽

Anal Region ◽

Age Related ◽

Old Rats ◽

Age Related Changes

AbstractThe recto-anal region is innervated by extrinsic and intrinsic nerves and a number of neuropeptides including substance P (SP) have been suggested to participate in the regulation of intestinal movements. We examined the age-related changes in the distribution of SP-immunoreactive nerve structures in the distal part of the rat large intestine. Using immunohistochemistry, the presence of SP was studied in fresh tissues from Wistar rats at different ages taken at three sampling sites, the distal rectum, anal canal and internal anal sphincter. In the 15-day old rats the myenteric plexus of the distal rectum and anal canal was well outlined by numerous SP-immunoreactive varicose nerve fibres encircling immunonegative perikarya. In the circular muscle layer, nerve fibres and small nerve bundles ran parallel to the muscle cells, while in the longitudinal muscle layer, only occasional nerve fibres were seen. At the level of the internal anal sphincter, no myenteric ganglia were present. Here, thin varicose fibers ran parallel to the smooth muscle cells. In the 3-month old rats, a larger number of intensely staining SP-immunoreactive nerve fibres were found and in the circular muscle layer, thicker nerve strands were observed. In the 26-month old rats, the density and staining intensity of SP-immunopositive nerve fibres in the myenteric plexus was lower than in the 3-month-old rats. Similar changes in the SP-immunostained fibres in the internal anal sphincter were observed. Degenerative alterations in SP-containing fibres during aging appear to play a role in ano-rectal motility and sphincter control.

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Glutathione metabolism in heart and liver of the aging rat

Biochemistry and Cell Biology ◽

10.1139/o94-010 ◽

1994 ◽

Vol 72 (1-2) ◽

pp. 58-61 ◽

Cited By ~ 35

Author(s):

M. Stio ◽

T. Iantomasi ◽

F. Favilli ◽

P. Marraccini ◽

B. Lunghi ◽

...

Keyword(s):

Rat Heart ◽

Reduced Glutathione ◽

Age Groups ◽

Glutathione Metabolism ◽

Oxidized Glutathione ◽

Glutathione S Transferase ◽

Age Related ◽

Glutamylcysteine Synthetase ◽

Old Rats ◽

Glucose 6 Phosphate Dehydrogenase

A comprehensive study on glutathione metabolism in rat heart and liver as a function of age was performed. In the heart, reduced glutathione, total glutathione, and the glutathione redox index showed a decrease during aging, while oxidized glutathione levels increased in 5-month-old rats with respect to the young animals and remained quite constant in 14- and 27-month-old rats. In the liver, the highest levels of reduced glutathione were found in the 2-month-old rats, while oxidized glutathione reached a peak at 5 months. Glutathione-associated enzymes showed age-related changes. Glutathione peroxidase, unaffected by aging in the heart, decreased in the liver of the 27-month-old rats. In the heart and the liver, the highest values of glutathione S-transferase were found at 5 months and 27 months, respectively. Glucose-6-phosphate dehydrogenase followed a similar trend in both heart and liver. Glutathione reductase also showed the same behaviour in heart and in liver, increasing in old rats with respect to the other age groups. A decrease in γ-glutamylcysteine synthetase was found in the heart and liver of 27-month-old rats in comparison with the 2-month-old ones. In conclusion, a decreased antioxidant capability has been demonstrated in both heart and liver of old rats.Key words: glutathione metabolism, age, rat heart, rat liver.

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Age-Related Changes in the Thyroid Hormone Effects on Malondialdehyde-Modified Proteins in the Rat Heart

Proceedings of The Society for Experimental Biology and Medicine ◽

10.1111/j.1525-1373.1999.09995.x ◽

1999 ◽

Vol 222 (1) ◽

pp. 59-64 ◽

Cited By ~ 5

Author(s):

Joe Chehade ◽

Joohee Kim ◽

Jacob L. Pinnas ◽

Arshag D. Mooradian

Keyword(s):

Thyroid Hormone ◽

Rat Heart ◽

Age Related ◽

Hormone Effects ◽

Modified Proteins ◽

Age Related Changes

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Circulating hyaluronan levels in the rodent: effects of age and diet

AJP Cell Physiology ◽

10.1152/ajpcell.1995.268.4.c952 ◽

1995 ◽

Vol 268 (4) ◽

pp. C952-C957 ◽

Cited By ~ 4

Author(s):

J. Yannariello-Brown ◽

S. H. Chapman ◽

W. F. Ward ◽

T. C. Pappas ◽

P. H. Weigel

Keyword(s):

Fish Meal ◽

Caloric Intake ◽

Protein Source ◽

Male Rats ◽

Semisynthetic Diet ◽

Fischer 344 ◽

Age Related ◽

Old Rats ◽

Ad Libitum ◽

Age Related Changes

Circulating hyaluronan (HA) levels were investigated as a function of age and diet in Fischer 344 male rats. A biphasic pattern of age-related changes was observed in rats fed ad libitum a diet in which the protein source was soya/fish meal. HA levels in 3- to 6- and 22- to 29-mo-old rats were not statistically different. However, HA levels in 12- to 20-mo-old rats were 10-29% of the levels in younger or aged adults. HA levels were also measured in rats fed ad libitum a semisynthetic diet in which the protein source was hydrolyzed casein. Whereas the two colonies exhibited similar biphasic age-related changes, HA levels differed 4- to 20-fold at every age examined. Caloric restriction affected HA levels in 19-mo-old casein-fed rats; HA levels were 2.3 times higher than age-matched controls and were not statistically different from young or aged animals. Serum and plasma HA levels were identical in the same individuals at all ages tested. These data suggest that HA turnover and metabolism in the rat are affected by age, dietary composition, and caloric intake.

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