Simultaneous Estimation of Plasma Volume, Red Cell Volume and Thiocyanate Space in Unanesthetized Normal and Splenectomized Rats

1956 ◽  
Vol 185 (3) ◽  
pp. 441-445 ◽  
Author(s):  
Kee-Chang Huang ◽  
James H. Bondurant
Keyword(s):  
Body Weight ◽  
Cell Volume ◽  
Plasma Volume ◽  
Albino Rats ◽  
Simultaneous Estimation ◽  
Red Cell ◽  
Total Blood Volume ◽  
Total Blood ◽  
Red Cell Volume ◽  
The Common

A method for simultaneous estimation of plasma volume, red cell volume and thiocyanate space in unanesthetized rats utilizing a polyethylene catheter in the common carotid artery with T-1824, P32-tagged red cells, and NaSCN was perfected and determinations were performed on 77 male albino rats. Determinations of plasma volume using T-1824 or I131-serum albumin in the same animal gave essentially identical results. Total blood volume was calculated as the sum of P32-red cell volume and T-1824-plasma volume and was found to be 5.75 ml/100 gm body weight in normal rats and 5.61 ml/100 gm in splenectomized rats. There was a wide variation in the Fcell values of normal rats but little in splenectomized rats. This difference was highly significant and was attributed to the presence of the spleen. The average thiocyanate space was found to be 33.0 ml and 35.8 ml/100 gm in normal and splenectomized rats, respectively.

scholarly journals Blood volume in monoclonal gammopathy

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 301-307 ◽  
Author(s):  
R Alexanian
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Monoclonal Gammopathy ◽  
Red Cell ◽  
Total Blood Volume ◽  
Elevated Plasma ◽  
Total Blood ◽  
Red Cell Volume ◽  
The Relationship

Abstract The plasma volume, red cell volume, or both were measured in 170 normal, anemic, or polycythemic subjects. For anemic subjects without a serum protein abnormality or splenomegaly, the relationship between hematocrit and red cell volume was linear and predictable. In patients with a serum monoclonal globulin on electrophoresis, the plasma voluem was significantly increased for the hematocrit in 30%, and the total blood volume was increased in 45%. The frequency of an elevated plasma volume was higher in patients with a markedly increased level of monoclonal protein. Reductions of abnormal proteins with chemotherapy were associated with declines in plasma volume. For a specific concentration, the serum viscosity was highest in patients with IgM proteins and lowest in patients with IgG globulins. Marked elevations in viscosity were noted only in sera with macroglobulinemia or with more than 5 g/dl of IgG or IgA globulins.

Simultaneous determination of blood volume with Cr51 and T-1824 during hypothermia and rewarming

1959 ◽  
Vol 196 (4) ◽  
pp. 703-705 ◽  
Author(s):  
E. J. Fedor ◽  
B. Fisher
Keyword(s):  
Cell Volume ◽  
Simultaneous Determination ◽  
Blood Volume ◽  
Plasma Volume ◽  
Red Cell ◽  
Total Blood Volume ◽  
Total Blood ◽  
Red Cell Volume ◽  
Usual Feature

Dogs lightly anesthetized with ether, maintained between 23–24°C for 2 hours and rewarmed, were subjected to simultaneous determinations of red cell volume (Cr51) and plasma volume (T-1824). Red cell volume values were unchanged during the course of the experiment. Plasma volumes were significantly decreased during hypothermia and were transiently elevated during rewarming. Twenty-four hours after rewarming, total blood volume and plasma volume values were not significantly different from control values. It would seem that circulatory failure (‘rewarming shock’) is not a usual feature of rewarming following hypothermia of 2 hours duration.

scholarly journals Blood volume in monoclonal gammopathy

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 301-307 ◽  
Author(s):  
R Alexanian
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Monoclonal Gammopathy ◽  
Red Cell ◽  
Total Blood Volume ◽  
Elevated Plasma ◽  
Total Blood ◽  
Red Cell Volume ◽  
The Relationship

The plasma volume, red cell volume, or both were measured in 170 normal, anemic, or polycythemic subjects. For anemic subjects without a serum protein abnormality or splenomegaly, the relationship between hematocrit and red cell volume was linear and predictable. In patients with a serum monoclonal globulin on electrophoresis, the plasma voluem was significantly increased for the hematocrit in 30%, and the total blood volume was increased in 45%. The frequency of an elevated plasma volume was higher in patients with a markedly increased level of monoclonal protein. Reductions of abnormal proteins with chemotherapy were associated with declines in plasma volume. For a specific concentration, the serum viscosity was highest in patients with IgM proteins and lowest in patients with IgG globulins. Marked elevations in viscosity were noted only in sera with macroglobulinemia or with more than 5 g/dl of IgG or IgA globulins.

Blood Volume Measurement in Baboons: Simultaneous Estimation of Red Cell Volume and Plasma Volume

1985 ◽  
Vol 14 (6) ◽  
pp. 345-356
Author(s):  
Michael G. Garner ◽  
Andrew F. Phippard ◽  
John S. Horvath ◽  
Geoffrey G. Duggin ◽  
David J. Tiller
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Volume Measurement ◽  
Simultaneous Estimation ◽  
Red Cell ◽  
Red Cell Volume ◽  
Blood Volume Measurement

STUDIES ON THE BLOOD VOLUME FOLLOWING HYPOPHYSECTOMY IN MAN

1963 ◽  
Vol 42 (4) ◽  
pp. 552-563 ◽  
Author(s):  
Thomas Falkheden ◽  
Björn Sjögren ◽  
Håkan Westling
Keyword(s):  
Body Weight ◽  
Replacement Therapy ◽  
Cell Volume ◽  
Blood Volume ◽  
Mammary Carcinoma ◽  
Red Cell ◽  
Total Blood Volume ◽  
Total Blood ◽  
Before And After ◽  
Total Haemoglobin

ABSTRACT Using 51Cr-labelled red cells, studies on the blood volume were performed before and after hypophysectomy in five patients (three cases of metastatic mammary carcinoma and two cases of acromegaly). In addition, studies were performed only after hypophysectomy in nine patients (two cases of metastatic mammary carcinoma and seven diabetic subjects). All patients were on replacement therapy with cortisone (17,21-dihydroxy-pregn-4-ene-3,11,20-trione) postoperatively, and several cases also received thyroid and sex hormones. Hypophysectomy was followed by a decrease in the red cell volume, the calculated total blood volume, and the total amount of haemoglobin in the patients studied before and after operation. The differences in calculated total blood volume and the total amount of haemoglobin between all hypophysectomized patients and a series of control subjects were analyzed on the basis of body weight. The total blood volume and total haemoglobin were significantly reduced in the hypophysectomized group.

scholarly journals The Blood Volume of Normal Women

Blood ◽  
1954 ◽  
Vol 9 (12) ◽  
pp. 1205-1207 ◽  
Author(s):  
G. R. WADSWORTH
Keyword(s):  
Body Weight ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Red Cell ◽  
Red Cell Volume ◽  
Normal Women ◽  
The Relationship

Abstract Measurements of red cell volume and plasma volume in eight normal women confirm that, in relation to body weight, red cell volume is distinctly lower in women than in men and plasma volume only slightly lower. The relationship between body hematocrit and venous hematocrit (0.90) was found not to be significantly different from that of men.

Plasma volume, cell volume, total blood volume and Fcells factor in the normal and splenectomized Sherman rat

1958 ◽  
Vol 196 (1) ◽  
pp. 188-192 ◽  
Author(s):  
Louise Wang
Keyword(s):  
Body Weight ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Simultaneous Measurement ◽  
Total Blood Volume ◽  
Total Blood ◽  
Unanesthetized Animals ◽  
Cell Volumes ◽  
Volume Cell

A method is described for the catheterization of the carotid of the rat which permits blood volume determinations to be made on unanesthetized animals. The cell volume (P32) and plasma volume (T-1824) were measured simultaneously on the day after catheterization. The average Fcells factor was 0.739 (S.D., 0.053) in 11 normal rats and 0.726 (S.D., 0.041) in 10 splenectomized rats. In 50 unanesthetized normal rats the plasma volume averaged 3.90 ml/100 gm body weight. The blood and cell volumes calculated by using the Fcells factor of 0.74 and the separately determined plasma trapping' factor of 0.95 averaged 5.93 ml/ 100 gm and 2.14 ml/100 gm, respectively. These values agree closely with those determined from the simultaneous measurement of cell and plasma volumes and also with the values obtained on 27 normal rats under ether.

Normal blood volumes in the horse

1964 ◽  
Vol 207 (1) ◽  
pp. 223-227 ◽  
Author(s):  
N. A. Marcilese ◽  
R. M. Valsecchi ◽  
H. D. Figueiras ◽  
H. R. Camberos ◽  
J. E. Varela
Keyword(s):  
Body Weight ◽  
Blood Volume ◽  
Plasma Volume ◽  
Red Cell ◽  
Total Blood Volume ◽  
Total Blood ◽  
Mean Values ◽  
The Mean ◽  
Race Horses ◽  
Blood Volumes

Blood volumes of 51 horses of three different breeds were determined by means of radioisotopes Cr51 and Fe59. The mean values obtained in 34 determinations for 31 Thoroughbred English race horses are as follows: total blood volume, 10.31; plasma volume, 6.33; and red cell volume 3.98 liters/100 kg body weight for a hematocrit of 42.7%. The results for 6 saddle horses are: 7.75, 5.25, and 2.53, respectively, for a hematocrit of 37.3%. The results in 14 draft horses are: 6.14, 4.35, and 1.82 for a hematocrit of 33.5%. The differences observed in the blood volume values of the three breeds are statistically significant. In our opinion, these differences are due to their physiological qualities, according to the breed.

scholarly journals The Body Hematocrit/Venous Hematocrit Ratio and the "Splenic Reservoir"

Blood ◽  
1961 ◽  
Vol 17 (1) ◽  
pp. 71-82 ◽  
Author(s):  
HUGH FUDENBERG ◽  
MARIO BALDINI ◽  
JOHN P. MAHONEY ◽  
WILLIAM DAMESHEK
Keyword(s):  
Cell Volume ◽  
Plasma Volume ◽  
Normal Subjects ◽  
The Body ◽  
Measured Parameter ◽  
Sodium Chromate ◽  
Red Cell ◽  
Total Blood ◽  
Red Cell Volume ◽  
Cell Plasma

Abstract 1. Red cell volume and plasma volume were measured by the radioactive sodium chromate and the chromic chloride method respectively in 37 patients. 2. The body hematocrit/venous hematocrit (BH/VH) ratio was found to be constant in normal subjects and in patients in whom there was neither splenomegaly nor edema. The ratio was 0.896 ± 0.039. 3. In splenectomized patients the BH/VH ratio was normal indicating that in man, in normal conditions, the spleen does not have the function of a significant blood reservoir with raised cell/plasma ratio. 4. In patients with splenomegaly the BH/VH ratio was found to be significantly increased in direct proportion to the degree of splenic enlargement, indicating an increased concentration of erythrocytes in the enlarged spleen. 5. In the presence of extravascular fluid retention caused by prolonged prednisone treatment the BH/VH ratio was lower than normal. 6. It was concluded that in patients with splenomegaly or edema measurement of either red cell volume or plasma volume alone in conjunction with the "corrected" venous hematocrit does not permit valid assessment of the non-measured parameter nor of the total blood volume.

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