Perfusion of rat adipose tissue

1963 ◽  
Vol 205 (2) ◽  
pp. 405-412 ◽  
Author(s):  
André Robert ◽  
Robert O. Scow
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Protein Composition ◽  
Fat Pad ◽  
Edema Formation ◽  
Fatty Acid Release ◽  
Flow Through ◽  
Acid Release ◽  
Rat Adipose Tissue ◽  
Fasted Rats

A technique is described for perfusing the parametrial fat pad of the rat. The rate of flow through the tissue was dependent on temperature, pressure, and the protein composition of the perfusing fluid. The latter also had a pronounced effect on the rate of edema formation in the tissue. The rate of fatty acid release by perfused adipose tissue was affected by the nutritional state of the fat donor and by the composition of the perfusing fluid. Tissue from fed rats did not release fatty acids. Adipose tissue from fasted rats released fatty acids when the tissue was perfused with diluted blood (1.10) or 4% albumin solution but not when perfused with 8% albumin. Perfused tissue released fatty acids at a much faster rate than did incubated tissue. Diluted blood and serum inhibited the release of fatty acids by incubated adipose tissue.

scholarly journals The effect of dietary lipids on lipolysis in rat adipose tissue

1975 ◽  
Vol 33 (2) ◽  
pp. 291-297 ◽  
Author(s):  
P. W. Larking ◽  
E. R. Nye
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Dietary Fat ◽  
Dietary Lipids ◽  
Prostaglandin E ◽  
Adipose Tissue Lipolysis ◽  
Rat Adipose Tissue ◽  
Fasted Rats

1. Rats were fed for 8 weeks on one of five diets differing in the amount of fatty acids 18:1, 18:2 and 18:3. Lipolysis, in vitro, of epididymal fat from fed and fasted rats was measured both basally and in the presence of noradrenaline with and without prostaglandin E12. Lipolysis was markedly influenced by the type of dietary fat. In particular, lipolysis in adipose tissue from rats given diets rich in the fatty acid 18:3 was higher than in the rats given diets containing 18:23. Results showing the effects of fasting on adipose tissue lipolysis are also presented4. The results are discussed in relation to the known effects of unsaturated fats on hyper-plasia and protein synthesis in adipose tissue and on the possible role of prostaglandins.

Glucose metabolism and mobilization of fatty acids by adipose tissue from obese mice

1961 ◽  
Vol 201 (1) ◽  
pp. 19-22 ◽  
Author(s):  
Bernard Leboeuf ◽  
Serene Lochaya ◽  
Nicole Leboeuf ◽  
Francis C. Wood ◽  
Jean Mayer ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Glucose Metabolism ◽  
Swiss Mice ◽  
Adipose Tissue In Vitro ◽  
Fatty Acid Release ◽  
Acid Release

Free fatty acid mobilization and glucose metabolism to CO2, to glyceride-glycerol, to fatty acids and to glycogen by adipose tissue in vitro were studied in genetically obese hyperglycemic mice (O-H), in goldthioglucose-injected obese Swiss mice and in their respective nonobese littermates. Tissue from O-H mice metabolizes less glucose than tissue from their nonobese littermates in absence of added hormone or in the presence of insulin (0.1 unit/ml) or epinephrine (10–4 m). In addition, there is also a diminished ability for insulin to inhibit and for epinephrine to augment free fatty acid release. No such differences were observed between tissues from goldthioglucose-injected obese Swiss mice and tissues from their lean littermates. Possible significance of these in vitro results with regard to the pathogenesis of the obese hyperglycemic syndrome is discussed.

scholarly journals Biochemical actions of vasoactive peptide hormones. Time-synchronized activation of lipolysis and decreased fatty-acid release by bradykinin and angiotensin in the perfused rabbit kidney

1980 ◽  
Vol 192 (1) ◽  
pp. 127-131 ◽  
Author(s):  
M Schwartzman ◽  
A Raz
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Time Lag ◽  
Rabbit Kidney ◽  
Subsequent Decrease ◽  
Fatty Acid Release ◽  
Initial Activation ◽  
Acid Release ◽  
Sequential Processes ◽  
Vasoactive Peptide

Bradykinin and angiotensin administered to the isolated perfused rabbit kidney activate two sequential processes: (1) a selective release of the prostaglandin precursor arachidonate with concomitant partial conversion of the arachidonate into prostaglandin E2; (2) activation of a process that leads to decreased release of all fatty acids in the perfusate. There is a time lag of approx. 1 min between the initial activation of the arachidonate-specific deacylation reaction that is coupled to prostaglandin generation, and the subsequent decrease in the release of all fatty acids. This synchronized cycle provides for instant generation of required amounts of prostaglandins and at the same time serves to conserve cellular arachidonate.

Epinephrine-insulin antagonism on free fatty acid release

1961 ◽  
Vol 201 (5) ◽  
pp. 815-818 ◽  
Author(s):  
John J. Spitzer ◽  
William T. McElroy
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Blood Sugar ◽  
Drug Administration ◽  
Hepatic Uptake ◽  
Fatty Acid Release ◽  
Plasma Ffa ◽  
Acid Release

The effects of epinephrine or norepinephrine were studied in dogs receiving insulin plus glucose prior to and during administration of the amine. Epinephrine caused a significantly smaller elevation of free fatty acids (FFA) with than without insulin plus glucose administration. Blood sugar responses were quantitatively similar. Epinephrine increased both hepatic uptake of FFA and hepatic release of glucose; these changes were similar to the ones found previously in dogs not receiving insulin plus glucose. The action of norepinephrine on elevating plasma FFA was only slightly and not significantly affected by the administration of insulin plus glucose. When the order of drug administration was reversed, infusion of insulin plus glucose lowered plasma FFA levels and hepatic FFA uptake in animals already receiving either epinephrine or nonepinephrine.

Effect of cold stress on lipogenesis by adipose tissue

1960 ◽  
Vol 198 (5) ◽  
pp. 1123-1125 ◽  
Author(s):  
E. J. Masoro ◽  
Edith Porter ◽  
Judith Patkin
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Cold Stress ◽  
Incubation Medium ◽  
Acetate Metabolism ◽  
Hepatic Lipogenesis ◽  
Acetate Oxidation ◽  
Fat Pad ◽  
Striking Finding ◽  
Epididymal Fat

The effect of cold stress on acetate metabolism by adipose tissue was investigated. Cold stress did not affect the ability of the epididymal fat pad to oxidize acetate-1-C14 to C14O2. The addition of unlabeled glucose to the incubation medium did not influence the rate of acetate oxidation in the case of adipose tissue obtained from either control or cold-stressed rats. In the absence of unlabeled glucose, more fatty acids from acetate-1-C14 were synthesized by the adipose tissue from control rats than by that from cold-stressed rats, although very little was synthesized by either. The addition of unlabeled glucose to the incubation medium at the physiologic concentration of 100 mg % caused the adipose tissue from both normal and cold-stressed rats to form fatty acids at high rates. It is a striking finding that cold stress, which almost abolishes hepatic lipogenesis, does not appreciably alter adipose tissue lipogenesis.

Sign in / Sign up

Export Citation Format

Share Document