Quantitative trait locus mapping of genes regulating pulmonary PKC activity and PKC-α content

2000 ◽  
Vol 279 (2) ◽  
pp. L326-L332 ◽  
Author(s):  
Lori D. Dwyer-Nield ◽  
Beverly Paigen ◽  
Stephanie E. Porter ◽  
Alvin M. Malkinson
Keyword(s):  
Quantitative Trait Locus ◽  
Likelihood Ratio ◽  
Quantitative Trait ◽  
Single Gene ◽  
Inbred Mouse ◽  
Likelihood Ratio Statistic ◽  
Mouse Strains ◽  
Chromosome 11 ◽  
Major Locus ◽  
Trait Locus

Strain A/J mice, which are predisposed to experimentally induced asthma and adenocarcinoma, have the lowest pulmonary protein kinase (PK) C activity and content among 22 inbred mouse strains. PKC in neonatal A/J mice is similar to that in other strains, so this difference reflects strain-dependent postnatal regulation. PKC activity is 60% higher in C57BL/6J (B6) than in A/J lungs, and the protein and mRNA concentrations of PKC-α, the major pulmonary PKC isozyme, are two- to threefold higher in B6 mice. These differences result from more than a single gene as assessed in F1, F2, and backcross progeny of B6 and A/J parents. Quantitative trait locus (QTL) analysis of 23 A×B and B×A recombinant inbred strains derived from B6 and A/J progenitors indicates a major locus regulating lung PKC-α content that maps near the Pkcα structural gene on chromosome 11 ( D11MIT333; likelihood ratio statistic = 12.5) and a major locus controlling PKC activity that maps on chromosome 3 ( D3MIT19; likelihood ratio statistic = 15.4). The chromosome 11 QTL responsible for low PKC-α content falls within QTLs for susceptibilities to lung tumorigenesis and ozone-induced toxicity.

Quantitative Trait Loci on Chromosomes 10 and 11 Influencing Mandible Size of SMXA RI Mouse Strains

2002 ◽  
Vol 81 (7) ◽  
pp. 501-504 ◽  
Author(s):  
A. Dohmoto ◽  
K. Shimizu ◽  
Y. Asada ◽  
T. Maeda
Keyword(s):  
Quantitative Trait Locus ◽  
Quantitative Trait ◽  
Major Gene ◽  
Recombinant Inbred Strain ◽  
Mouse Strains ◽  
Chromosome 11 ◽  
Significant Qtls ◽  
Mandible Length ◽  
Trait Locus ◽  
Mandible Size

Predicting the mandible size before the termination of growth of the maxillofacial bones is essential in pedodontics as well as for the predictions needed for genetic analysis. Here, Quantitative Trait Locus (QTL) analysis was used to detect the chromosomal regions responsible for the mandible length between the menton and gonion in an SMXA recombinant inbred strain of mice. Around the region 60 cM from the centromere in chromosome 10, the logarithm of the odds score showed a higher than suggestive level. Around the regions 13 cM and 16 cM in chromosome 11, two significant QTLs were detected. Analysis of genotypes from loci corresponding to those QTLs revealed a large mandible when the region between the markers Hba and D11Mit163 and D10Mit70 and D10Mit136 indicated the genotype from the A/J and SM/J alleles, respectively. These results suggest that the major gene(s) responsible for mandible length are located in these regions.

scholarly journals IDENTIFICATION OF A MAJOR QUANTITATIVE TRAIT LOCUS CONFERRING RICE BLAST RESISTANCE USING RECOMBINANT INBRED LINES

2013 ◽  
Vol 11 (1) ◽  
pp. 1
Author(s):  
Sobrizal Sobrizal ◽  
Masdiar Bustamam ◽  
Carkum Carkum ◽  
Ahmad Warsun ◽  
Soeranto Human ◽  
...  
Keyword(s):  
Quantitative Trait Locus ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Blast Resistance ◽  
Blast Disease ◽  
Chromosome 11 ◽  
Resistant Gene ◽  
Trait Locus ◽  
Ri Lines ◽  
Major Qtl

Blast disease caused by Pyricularia oryzae is one of the limiting factors for rice production world wide. The use of resistant varieties for managing blast disease is considered as the most eco-friendly approaches. However, their resistances may be broken down within a few years due to the appearance of new virulent blast races in the field. The objective of the present study was to identify the quantitative trait locus (QTL) conferring resistance to blast disease using 126 recombinant inbred (RI) lines originated from a crossing of a durably resistant upland rice genotype (Laka) and a highly susceptible rice accession cultivar (Kencana Bali). The RI population was developed through a single seed descent method from 1997 to 2004. Resistance of the RI lines was evaluated for blast in an endemic area of Sukabumi, West Java, in 2005. Disease intensity of the blast was examined following the standard evaluation system developed by the International Rice Research Institute (IRRI). At the same year the RI lines were analyzed with 134 DNA markers. Results of the study showed that one major QTL was found to be associated with blast resistance, and this QTL was located near RM2136 marker on the long arm of chromosome 11. This QTL explained 87% of the phenotypic variation with 37% additive effect. The map position of this QTL differed from that of a partial resistant gene, Pi34, identified previously on chromosome 11 in the Japanese durably resistant variety, Chubu 32. The QTL, however, was almost at the same position as that of the multiple allele-resistant gene, Pik. Therefore, an allelic test should be conducted to clarify the allelic relationship between QTL identified in this study and the Pik. The RI lines are the permanent segregating population that could be very useful for analysing phenotypic variations of important agronomic traits possibly owned by the RI lines. The major QTL identified in this study could be used as a genetic resource in improvement of rice varieties for blast resistance in Indonesia

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