Regional changes in adipose tissue blood flow and metabolism in rats after a meal

1989 ◽  
Vol 257 (4) ◽  
pp. R711-R716 ◽  
Author(s):  
D. B. West ◽  
W. A. Prinz ◽  
M. R. Greenwood
Keyword(s):  
Blood Flow ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Tissue Blood Flow ◽  
Lipoprotein Lipase Activity ◽  
Fat Depots ◽  
Adipose Tissue Blood Flow ◽  
Regional Specificity ◽  
Adipose Tissue Lipoprotein Lipase

Adipose tissue blood flow was measured in five depots, and plasma concentrations of glucose, insulin, and triglyceride were measured at 0, 15, 30, and 45 min after the start of a meal in unanesthetized, freely moving rats. In addition, adipose tissue lipoprotein lipase activity was measured in four depots before and 45 min after the start of a meal. Plasma glucose was significantly elevated only at the 15-min time point, and while plasma triglyceride increased these changes did not reach significance. Plasma insulin was significantly elevated at all time points after a meal. Feeding resulted in a consistent decrease of adipose tissue blood flow expressed per gram wet weight of tissue. This decrease was maximal at 30 min after the start of feeding. The decrease in adipose tissue blood flow averaged 45% at 45 min after the start of feeding for the five depots evaluated. Lipoprotein lipase activity significantly increased in the retroperitoneal and mesenteric fat depots at 45 min after the meal start, but did not change in the epididymal or dorsal subcutaneous fat depots. These results suggest that a decrease in adipose tissue blood flow is a normal result of a meal in the rat. The regional specificity of changes in adipose tissue lipoprotein lipase activity supports the concept of regional specificity of function for adipose tissue and suggests that the mesenteric and retroperitoneal depots are particularly important for the storage of triglycerides immediately after a meal.

scholarly journals Post-translational regulation of lipoprotein lipase activity in adipose tissue

1978 ◽  
Vol 176 (3) ◽  
pp. 865-872 ◽  
Author(s):  
P Ashby ◽  
D P Bennett ◽  
I M Spencer ◽  
D S Robinson
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Translational Regulation ◽  
Progressive Increase ◽  
Lipoprotein Lipase Activity ◽  
Dependent Part ◽  
Adipose Tissue Lipoprotein Lipase ◽  
Energy Dependent

Changes in adipose-tissue lipoprotein lipase activity that are independent of protein synthesis were investigated in an incubation system in vitro. Under appropriate conditions at 25 degrees C a progressive increase in the enzyme activity occurs that is energy-dependent. Part of the enzyme is rapidly inactivated when the tissue is incubated with adrenaline or adrenaline plus theophylline. The mechanism of this inactivation appears to be distinct from, and to follow, the activation of the enzyme. A hypothesis is presented to account for the results in terms of an activation of the enzyme during obligatory post-translational processing and a catecholamine-regulated inactivation of the enzyme as an alternative to secretion from the adipocyte.

scholarly journals Adipose-tissue lipoprotein lipase activity and cellularity in the genetically obese Zucker rat (fa/fa) (Short Communication)

1973 ◽  
Vol 132 (3) ◽  
pp. 633-635 ◽  
Author(s):  
P. de Gasquet ◽  
E. Péquignot ◽  
D. Lemonnier ◽  
A. Alexiu
Keyword(s):  
Adipose Tissue ◽  
Surface Area ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Enzyme Activities ◽  
Short Communication ◽  
Zucker Rat ◽  
Lipoprotein Lipase Activity ◽  
Obese Zucker Rat ◽  
Adipose Tissue Lipoprotein Lipase

The lipoprotein lipase activity per adipocyte was increased in the genetically obese rat (fa/fa). However, there was no difference between obese and lean animals when the enzyme activities were related to adipocyte surface area. The possible implications of the findings are discussed.

Adipose tissue lipoprotein lipase activity in type III hyperlipoproteinemia

Metabolism ◽  
1979 ◽  
Vol 28 (11) ◽  
pp. 1122-1126 ◽  
Author(s):  
Andrew P. Goldberg ◽  
Deborah M. Applebaum-Bowden ◽  
William R. Hazzard
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipoprotein Lipase Activity ◽  
Type Iii ◽  
Type Iii Hyperlipoproteinemia ◽  
Adipose Tissue Lipoprotein Lipase

Serum Activation of Lipoprotein Lipase from Sheep Adipose Tissue

1985 ◽  
Vol 38 (1) ◽  
pp. 131
Author(s):  
RK Tume ◽  
RF Thornton
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipoprotein Lipase Activity ◽  
Nutritional Restriction ◽  
Adipose Tissue Lipoprotein Lipase ◽  
Increased Activity ◽  
Substrate Concentrations

The effects of species and plane of nutrition on serum activation of sheep adipose tissue lipoprotein lipase were studied over a range of substrate (triolein) concentrations. Serum, either from two species or from the same species on a different plane of nutrition, had differing effects on adipose tissue lipoprotein lipase activity. Serum from fed sheep was more effective than serum from fed rats in activating sheep adipose tissue lipoprotein lipase at low substrate concentrations. Serum taken from sheep on a restricted plane of nutrition, stimulated adipose tissue lipoprotein lipase activity at physiological substrate concentrations. The increased activity promoted by the factor(s) present in serum would ensure that those tissues (e.g. cardiac and skeletal muscle) which continue to synthesize lipoprotein lipase during fasting or nutritional restriction, are able to assimilate the relatively low amounts of circulating triacylglycerol.

scholarly journals Interaction of dietary carbohydrate and fat in the regulation of hepatic and extrahepatic lipogenesis in the rat

1988 ◽  
Vol 59 (2) ◽  
pp. 233-241 ◽  
Author(s):  
Gene R. Herzberg ◽  
Maria Rogerson
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Acid Synthesis ◽  
Lipoprotein Lipase Activity ◽  
Lipogenic Enzymes ◽  
Total Body ◽  
Adipose Tissue Lipoprotein Lipase ◽  
Hepatic Synthesis ◽  
Maize Oil

1. In order to examine the interaction of dietary fat and carbohydrate in the regulation of lipid metabolism, we have studied hepatic and extrahepatic lipogenesis, and adipose tissue lipoprotein lipase (EC 3.1.1.34) in rats fed on one of the following diets: a fructose-based diet containing 0 (F0) or 150 g maize oil (F15)/kg, or a glucose based diet containing 0 (G0) or 150 g maize oil (G15)/kg.2. The rats were meal-fed on the diets for 2 weeks after which the activities of a number of hepatic ‘lipogenic’ enzymes were measured and the activity of epididymal-fat-pad lipoprotein lipase. The activities of the lipogenic enzymes were: F0 > G0 > G15 > F15. Lipoprotein lipase activity was F0 = G0 = F15 = G15. The percentage of total body fatty acid synthesis which occurred in the liver was F0 > G0 > F15 > G15.3. We conclude that fructose-induced hypertriglyceridaemia is primarily a result of the increased hepatic synthesis rather than decreased adipose-tissue lipoprotein lipase activity.

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