Passive length-force properties of senescent diaphragm: relationship with collagen characteristics

1994 ◽  
Vol 76 (6) ◽  
pp. 2680-2685 ◽  
Author(s):  
L. E. Gosselin ◽  
D. A. Martinez ◽  
A. C. Vailas ◽  
G. C. Sieck
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Diaphragm Muscle ◽  
Passive Force ◽  
Collagen Concentration ◽  
Chromatography Analysis ◽  
Fischer 344 ◽  
Relationship Of ◽  
Force Relationship

The purpose of this study was to examine the effect of aging on collagen concentration and extent of nonreducible collagen cross-linking as well as with the passive length-force relationship of the diaphragm muscle. Midcostal diaphragm muscle strips from young (6 mo) and senescent (24 mo) Fischer 344 rats were perfused in a tissue bath containing mammalian Ringers solution (25 degrees C) aerated with 95% O2–5% CO2. The segments were lengthened and shortened from 85 to 115% of optimal length (L(o)) at a constant velocity (0.6 L(o)/s), and the passive force was measured. Hydroxyproline (HYP) and the mature nonreducible collagen cross-link, hydroxylysylpyridinoline (HP), were measured by high-pressure liquid chromatography. The resting force at Lo did not differ between young and senescent diaphragm muscles. However, the senescent diaphragm exhibited greater passive force compared with the young (P < 0.05) at lengths > 110% of L(o). High-pressure liquid chromatography analysis revealed a higher concentration of HYP in the senescent compared with the young diaphragm (9.32 +/- 0.83 and 6.59 +/- 0.78 g HYP/mg dry wt, respectively; P < 0.05). Additionally, the content of HP was greater in the senescent compared with the young diaphragm (1.16 +/- 0.05 and 0.91 +/- 0.05 mol HP/mol collagen, respectively; P < 0.05). These results suggest that diaphragm collagen metabolism, maturation, and the passive length-force characteristics of the muscle are altered with senescence.

Changes in diaphragm muscle collagen gene expression after acute unilateral denervation

1995 ◽  
Vol 79 (4) ◽  
pp. 1249-1254 ◽  
Author(s):  
L. E. Gosselin ◽  
G. C. Sieck ◽  
R. A. Aleff ◽  
D. A. Martinez ◽  
A. C. Vailas
Keyword(s):  
Mrna Level ◽  
Diaphragm Muscle ◽  
Passive Force ◽  
Cross Link ◽  
Optimal Length ◽  
Collagen Concentration ◽  
Fischer 344 ◽  
Length Relationship ◽  
Relationship Of

The purpose of the present study was to examine the effects of acute (3 days) unilateral diaphragm denervation (DNV) on 1) levels of alpha 1(I) and alpha 1(III) procollagen mRNA; 2) collagen concentration [hydroxyproline (HYP)]; 3) amount of the nonreducible collagen cross-link hydroxylysylpyridinoline (HP); and 4) the passive force-length relationship of the muscle. The levels of alpha 1(I) and alpha 1(III) procollagen mRNA, HYP concentration, and amount of HP were measured in muscle segments from the midcostal region of DNV and intact (INT) hemidiaphragms of adult male Fischer 344 rats (250–300 g). The in vitro passive force-length relationship of DNV and INT hemidiaphragm was determined by lengthening and shortening the diaphragm muscle segments from 85 to 115% of optimal length at a constant velocity (0.6 optimal length/s). Three days after DNV, the level of alpha 1(I) procollagen mRNA was increased over 15-fold in the DNV hemidiaphragm compared with INT (P < 0.05), whereas the level of alpha 1(III) procollagen mRNA was increased by approximately sixfold in the DNV hemidiaphragm compared with INT (P < 0.05). Collagen (HYP) concentration did not differ between groups, averaging 8.7 and 8.9 micrograms/mg dry wt for the DNV and INT hemidiaphragms, respectively. In addition, there was no difference in the amount of the mature nonreducible collagen cross-link HP between the DNV and INT hemidiaphragms (0.66 vs. 0.76 mole HP/mole collagen, respectively). The amount of passive force developed during lengthening did not differ between DNV and INT hemidiaphragms. These data indicate that acute DNV of the hemidiaphragm is associated with an increase in the mRNA level of the two principal fibrillar collagen phenotypes in skeletal muscle. However, despite extensive muscle remodeling, the passive force-length relationship of the DNV hemidiaphragm is unaffected compared with the INT muscle.

Incidence of Patulin in Apple, Pear, and Mixed Fruit Products Marketed in New South Wales

1992 ◽  
Vol 55 (10) ◽  
pp. 796-802 ◽  
Author(s):  
KATARINA BURDA
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
New South ◽  
New South Wales ◽  
Detection Limits ◽  
Chromatography Analysis ◽  
South Wales ◽  
Juice Concentrate

Three hundred twenty-eight (328) apple, pear, and mixed fruit products including juices, sauces, purees, jellies, diced apples, and apple pulps from 38 Australian producers were analyzed for patulin using the technique of high pressure liquid chromatography analysis. Patulin was detected in 75 of 258 juice and juice concentrate samples ranging from 5 to 50 μg/L and in 73 samples ranging from 51 to 1130 μg/L. Of 70 samples other than juices, patulin was detected in 18 samples at levels below 50 μg/kg of patulin. The detection limits for patulin in juice and other products were 5 μg/L and 5 μg/kg, respectively.

scholarly journals Polyamine concentrations in bone marrow aspirates of children with leukemia and other malignancies

Blood ◽  
1976 ◽  
Vol 47 (4) ◽  
pp. 695-701
Author(s):  
O Rennert ◽  
T Miale ◽  
J Shukla ◽  
D Lawson ◽  
J Frias
Keyword(s):  
Bone Marrow ◽  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Marked Elevation ◽  
Chromatography Analysis ◽  
Polyamine Analysis

High-pressure liquid chromatography analysis of polyamines in bone narrow from leukemic and nonleukemic subjects demonstrated increased concentrations of putrescine, spermidine, and spermine associated with increased cellularity. The most striking abnormality was the marked elevation of putrescine. Bone marrow polyamine analysis may be an adjunct for evaluation of leukemia patients.

scholarly journals Polyamine concentrations in bone marrow aspirates of children with leukemia and other malignancies

Blood ◽  
1976 ◽  
Vol 47 (4) ◽  
pp. 695-701 ◽  
Author(s):  
O Rennert ◽  
T Miale ◽  
J Shukla ◽  
D Lawson ◽  
J Frias
Keyword(s):  
Bone Marrow ◽  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Marked Elevation ◽  
Chromatography Analysis ◽  
Polyamine Analysis

Abstract High-pressure liquid chromatography analysis of polyamines in bone narrow from leukemic and nonleukemic subjects demonstrated increased concentrations of putrescine, spermidine, and spermine associated with increased cellularity. The most striking abnormality was the marked elevation of putrescine. Bone marrow polyamine analysis may be an adjunct for evaluation of leukemia patients.

scholarly journals Cell-free conversion of isopenicillin N into deacetoxycephalosporin C by Cephalosporium acremonium mutant M-0198

1981 ◽  
Vol 194 (2) ◽  
pp. 649-651 ◽  
Author(s):  
J E Baldwin ◽  
J W Keeping ◽  
P D Singh ◽  
C A Vallejo
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Cephalosporium Acremonium ◽  
Resistant Material ◽  
Free System ◽  
Cell Free System ◽  
Chromatography Analysis ◽  
A Cell ◽  
Isopenicillin N

In a cell-free system prepared by lysis of protoplasts of Cephalosporium acremonium mutant M-0198, isopenicillin N was converted into a penicillinase-resistant material that behaved like deacetoxycephalosporin C on high-pressure liquid chromatography analysis. This activity was found to be unstable to storage at -80 degrees C; 70-80% of the activity was lost after 1 day.

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