Simulated microgravity, psychic stress, and immune cells in men: observations during 120-day 6° HDT

2001 ◽  
Vol 90 (5) ◽  
pp. 1736-1743 ◽  
Author(s):  
A. Choukèr ◽  
M. Thiel ◽  
V. Baranov ◽  
D. Meshkov ◽  
A. Kotov ◽  
...  
Keyword(s):  
Immune Cells ◽  
Polymorphonuclear Leukocytes ◽  
Nk Cell ◽  
Stress Test ◽  
Suppressor Cells ◽  
Psychological State ◽  
T Helper ◽  
Long Duration ◽  
A Current ◽  
T Suppressor Cells

Because 6° head-down tilt (HDT) is an established method to mimic low gravity on earth, the aim of the present study was to determine the effects of 120-day HDT on psychic stress and peripheral blood immune cells in six healthy male volunteers. Psychological state was assessed by a current stress test, and cortisol was measured in saliva. During HDT, all volunteers developed psychic stress, and the diurnal rhythm of cortisol secretion was significantly altered. In addition, urine excretion of dopamine and norepinephrine increased. The innate part of the immune response was activated, as evidenced by the increase in the expression of β2-integrins on polymorphonuclear leukocytes and a rise in the number of circulating natural killer (NK) cell lymphocytes. The ratio of T-helper to T-cytotoxic and T-suppressor cells decreased, whereas no changes in T and B lymphocytes were observed. Plasma levels of interleukin-6 increased significantly and returned to basal levels after the end of the HDT period. Thus 6° HDT appears to be a valid model to induce psychic stress and neuroendocrine-related changes in the immune system, changes that might also be encountered by astronauts and cosmonauts during long-duration spaceflights.

Effects of confinement (110 and 240 days) on neuroendocrine stress response and changes of immune cells in men

2002 ◽  
Vol 92 (4) ◽  
pp. 1619-1627 ◽  
Author(s):  
A. Choukèr ◽  
L. Smith ◽  
F. Christ ◽  
I. Larina ◽  
I. Nichiporuk ◽  
...  
Keyword(s):  
Stress Test ◽  
Suppressor Cells ◽  
Delayed Type Hypersensitivity ◽  
Immunological Parameters ◽  
Enhanced Expression ◽  
Hypersensitivity Skin ◽  
Male Subjects ◽  
A Current ◽  
T Suppressor Cells

The aim of the study was to evaluate the effects of long-term confinement on stress-permissive neuroendocrine and immune responses in humans. Two groups of four male subjects were confined 240 days ( group 240) or 110 days ( group 110) in two space modules of 100 or 200 m3, respectively. During confinement, none of the volunteers developed psychic stress as could be examined and verified by a current stress test. However, in g roup 240 but not in group 110, the diurnal rhythm of cortisol secretion was slightly depressed and the urine excretion of norepinephrine significantly increased. The innate part of the immune system became activated as seen by a rise in the number of circulating granulocytes and the enhanced expression of β2-integrins. In contrast, the ratio of T-helper to T-suppressor cells decreased. All these effects, observed during confinement, were even more pronounced in both groups when values of endocrinological and immunological parameters were compared between before and 1 wk after the end of the confinement period. Hence, return to normal life exerts pronounced effects to a much higher degree, irrespective of how long or under which conditions individuals were confined. Because the delayed-type hypersensitivity skin reaction against recall antigens remained unaffected, it is to be presumed that confinement appears to induce distinct sympathoadrenergic activation and immunological changes but no clinically relevant immunosuppression.

scholarly journals Identification of lymphocyte subpopulations with a polymethine dye.

1988 ◽  
Vol 36 (7) ◽  
pp. 711-715 ◽  
Author(s):  
L Kass
Keyword(s):  
Suppressor Cells ◽  
Buffy Coat ◽  
Lymphocyte Subpopulations ◽  
Rapid Screening ◽  
T Helper ◽  
Yellow Orange ◽  
A Cell ◽  
Health And Disease ◽  
T Suppressor Cells ◽  
Polymethine Dye

Using the polymethine dye p-ethoxyphenyl-p-aminostyryl-1,3,3-trimethyl-3H-indolium chloride as an aqueous stain applied to specimens of peripheral blood or buffy coat fixed in FAA fixative, differential coloration of leukocytes was achieved using darkfield illumination. Neutrophils stained dark maroon and contained green granules, eosinophils contained bright blue granules, basophils revealed yellow and pink granules, and monocytes stained green with green and yellow vacuoles. In studies of purified lymphocyte subpopulations obtained in a cell sorter, T-helper cells stained red, T-suppressor cells were yellow orange, B-cells appeared yellow and often contained yellow annular structures in the cytoplasm, and natural killer (NK) cells stained green and contained large green granules. As a rapid screening technique for identification of T-helper and T-suppressor cells and their ratios in health and disease, the new polymethine stain may complement the more complex monoclonal antibody techniques for identification of these cells.

Induction and separation of antigen-dependent T helper and T suppressor cells in man

Nature ◽  
1978 ◽  
Vol 274 (5671) ◽  
pp. 586-587 ◽  
Author(s):  
ABRAHAM SHORE ◽  
HANS-MICHAEL DOSCH ◽  
ERWIN W. GELFAND
Keyword(s):  
Suppressor Cells ◽  
T Helper ◽  
T Suppressor Cells

EVALUATION OF ALLOSPECIFIC T HELPER, T CYTOTOXIC AND T SUPPRESSOR CELLS IN TRANSPLANT RECIPIENTS.

2000 ◽  
Vol 69 (Supplement) ◽  
pp. S206
Author(s):  
Nicole Suciu-Foca ◽  
Sorina Tugulea ◽  
Rodica Ciubotariu ◽  
Shuiping Jiang ◽  
Elvira Renna-Molajoni ◽  
...  
Keyword(s):  
Suppressor Cells ◽  
Transplant Recipients ◽  
T Helper ◽  
T Suppressor Cells

CFU-GEMM Infused Influences Immunologic Recovery in Children Older Than 7 years After Hematopoietic Stem Cell Transplantation.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4483-4483
Author(s):  
Katarzyna Drabko ◽  
Marta Choma ◽  
Agnieszka Zaucha-Prazmo ◽  
Beata Wojcik ◽  
Dorota Winnicka ◽  
...  
Keyword(s):  
Stem Cell ◽  
T Helper Cells ◽  
Suppressor Cells ◽  
T Helper ◽  
Hematopoietic Stem ◽  
Cd34 Cells ◽  
Group 2 ◽  
Graft Quality ◽  
T Suppressor Cells ◽  
Group 1

Abstract Abstract 4483 Introduction Reconstitution of immunity is considered as one of the most important factors predicting outcome after hematopoietic stem cell transplantation (HSCT). Age of the recipient as well as graft quality reflect on this process however this influence is not completely understood yet. In this study we aimed to evaluate the influence of graft quality on recovery of lymphocyte subpopulation. Patients and Methods The total number of 63 patients who underwent allogeneic transplantation in one center between May 2002 and November 2008, were included into the study. Median age of the patient was 8,6 (range 0.2-18) yrs. Patients were divided into 2 age groups: group 1 (n=26) children age 0,2-7 and group 2 (n=37) children >7 yrs. In all cases quality of graft were assessed by counting WBC, CD34 cells and CFU-GEMM. 45 patients (21 in group 1 and 24 in group 2) were available for evaluation of immune reconstitution: in those patients the absolute number of lymphocytes CD3/CD19 (B) cells, CD3/CD4 (T helper) cell, CD3/CD8 (T suppressor) cells and CD3/CD16+56 (NK) cells were measured 3 and 6 months after transplantation. Results Younger children (group 1) received higher median number of transplanted nucleated cells (4.9×108 vs 2.5×108, p=0.008), CD34 cells (5.6×106 vs 2.1×106, p=0.02) and CFU-GEMM (2.25×104 vs 1.17×104, p=0.01) than older ones (group 2). No differences were found in absolute values of B, T-helper, T-suppressor and NK cells at 3 and 6 months after transplantation (Table 1). In older children (group 2), but not in younger ones, the number of GFU-GEMM transplanted negatively correlated with number of T-suppressor cells 3 months post HSCT (p<0,05; r=-0,7) and positively with number of T-helper cells 6 moths after HSCT (p<0,05; r=0,73). Conclusions Number of CFU-GEMM infused influences immunologic recovery in children older than 7 years after HSCT. Graft quality correlates with number of T-suppressor cells and T-helper cells during first 6 months after HSCT and these changes may contribute to post transplant complications. Supported by grant MNiSW of Poland N407 117 35 Disclosures: No relevant conflicts of interest to declare.

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