Regulation of Body Fluid Volume: The Atrial Natriuretic Factor

The discovery of the atrial natriuretic factor is a major breakthrough for our understanding of the regulation of body fluid volume. It plays a major role in vasorelaxation and in hypertensive and edematous states. The progress made in the last two years is a testimony to the powers of the new analytical techniques for isolation and characterization of peptides.

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Degradation of atrial natriuretic factor by kidney cortex membranes. Isolation and characterization of the primary proteolytic product.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)60854-3 ◽

1987 ◽

Vol 262 (24) ◽

pp. 11623-11627 ◽

Cited By ~ 4

Author(s):

J A Koehn ◽

J A Norman ◽

B N Jones ◽

L LeSueur ◽

Y Sakane ◽

...

Keyword(s):

Atrial Natriuretic Factor ◽

Kidney Cortex ◽

Isolation And Characterization ◽

Natriuretic Factor ◽

Proteolytic Product ◽

Atrial Natriuretic

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Control of atrial natriuretic factor release in conscious dogs

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1986.251.5.r947 ◽

1986 ◽

Vol 251 (5) ◽

pp. R947-R956 ◽

Cited By ~ 1

Author(s):

K. M. Verburg ◽

R. H. Freeman ◽

J. O. Davis ◽

D. Villarreal ◽

R. C. Vari

Keyword(s):

Sodium Excretion ◽

Atrial Natriuretic Factor ◽

Isotonic Saline ◽

Extracellular Fluid ◽

Fluid Volume ◽

Extracellular Fluid Volume ◽

Conscious Dogs ◽

Base Line ◽

Natriuretic Factor ◽

Atrial Natriuretic

The aim of this study was to examine the changes in the concentration of plasma immunoreactive atrial natriuretic factor (iANF) that occur in response to expansion or depletion of the extracellular fluid volume in conscious dogs. The plasma iANF concentration was also measured postprandially after the ingestion of a meal containing 125 meq of sodium. Postprandial plasma iANF increased 45% (P less than 0.05) above the base-line concentration, and this increase was accompanied by a brisk natriuresis. After a low-sodium meal, however, plasma iANF and sodium excretion failed to increase. The plasma iANF concentration increased from 57 +/- 5 to 139 +/- 36 pg/ml (P less than 0.05) immediately after volume expansion with intravenous isotonic saline infusion (2.5% body wt) administered over a 30-min period; plasma iANF remained elevated at 90 +/- 14 pg/ml (P less than 0.05) for an additional 30 min before returning toward preinfusion levels. Plasma iANF decreased 45% from 78 +/- 17 to 43 +/- 7 pg/ml (P less than 0.05) in response to the administration of ethacrynic acid (2.0 mg/kg, iv bolus) that produced an estimated 15% depletion of intravascular volume. In additional experiments the infusion of synthetic alpha-human ANF at 100 and 300 ng X kg-1 X min-1 increased (P less than 0.05) both the plasma iANF concentration and the urinary excretion of iANF. This study demonstrates that the secretion of ANF is consistently influenced by changes in the extracellular fluid volume. Furthermore, the results support the concept that ANF functions to increase postprandial sodium excretion following the ingestion of a high-sodium meal.

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CHARACTERIZATION OF ATRIAL NATRIURETIC FACTOR RECEPTORS IN ADRENAL CORTEX, VASCULAR SMOOTH MUSCLE AND ENDOTHELIAL CELLS BY AFFINITY LABELING

Biomedical Research ◽

10.2220/biomedres.7.35 ◽

1986 ◽

Vol 7 (1) ◽

pp. 35-38 ◽

Cited By ~ 20

Author(s):

MASASHI SHINJO ◽

YUKIO HIRATA ◽

HIROMI HAGIWARA ◽

FUMIAKI AKIYAMA ◽

KAZUO MURAKAMI ◽

...

Keyword(s):

Endothelial Cells ◽

Smooth Muscle ◽

Vascular Smooth Muscle ◽

Adrenal Cortex ◽

Atrial Natriuretic Factor ◽

Natriuretic Factor ◽

Atrial Natriuretic

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A Possible Physiological Role of Atrial Natriuretic Peptide in Body Fluid Volume Regulation

Journal of Cardiovascular Pharmacology ◽

10.1097/00005344-198805006-00018 ◽

1988 ◽

Vol 13 ◽

pp. S62-S68

Author(s):

Yasunobu Hirata ◽

Masao Ishii ◽

Kazushige Fukui ◽

Hiroshi Hayakawa ◽

Shin-ichiro Namba ◽

...

Keyword(s):

Atrial Natriuretic Peptide ◽

Natriuretic Peptide ◽

Volume Regulation ◽

Body Fluid ◽

Physiological Role ◽

Fluid Volume ◽

Body Fluid Volume ◽

Fluid Volume Regulation ◽

Atrial Natriuretic

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Dehydration increases the density of C receptors for ANF on rat glomerular membranes

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1990.258.4.r1084 ◽

1990 ◽

Vol 258 (4) ◽

pp. R1084-R1088 ◽

Cited By ~ 2

Author(s):

M. C. Kollenda ◽

A. M. Vollmar ◽

G. A. McEnroe ◽

A. L. Gerbes

Keyword(s):

Binding Sites ◽

Atrial Natriuretic Factor ◽

Body Fluid ◽

Fluid Volume ◽

Control Group ◽

Receptor Density ◽

Lower Plasma ◽

Short Term ◽

Salt Loading ◽

Body Fluid Volume

The present study determined the presence of two types of binding sites for atrial natriuretic factor (ANF), the B and C receptor, on rat glomerular membranes. The effect of short-term salt loading and dehydration on these two receptor populations was investigated consecutively. Salt-loaded rats did not show significant changes in plasma ANF concentrations or in the number of ANF binding sites. Water-deprived rats presented significantly lower plasma ANF concentrations (22.0 +/- 1.9 vs. 34.4 +/- 3.8 fmol/ml, P less than 0.01) and an increase in total receptor density (1,860 +/- 398 vs. 987 +/- 143 fmol/mg protein) as compared with the control group. Differentiation of both receptor populations showed that it was the C receptors that accounted for this increase (1,772 +/- 369 vs. 901 +/- 151 fmol/mg protein, P less than 0.05), whereas B-receptor density was unchanged (89 +/- 31 vs. 87 +/- 44 fmol/mg protein). These data suggest that C receptors for ANF are affected by changes of body fluid volume.

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