Neutrophil and Monocyte CD64 and CD163 Expression in Critically Ill Neonates and Children with Sepsis: Comparison of Fluorescence Intensities and Calculated Indexes

Objective. To evaluate the expression of CD64 and CD163 on neutrophils and monocytes in SIRS with/without sepsis and to compare the diagnostic accuracy of CD64 and CD163 molecules expression determined as (1) mean fluorescence intensities (MFI) of CD64 and CD163; and (2) the ratio (index) of linearized MFI to the fluorescence signal of standardized beads.Patients and methods. Fifty-six critically ill neonates and children with systemic inflammatory response syndrome (SIRS) and suspected sepsis, classified into two groups: SIRS with sepsis (n=29) and SIRS without sepsis (n=27).Results. CD64 and CD163 MFI measured on neutrophils and monocytes were elevated in patients with SIRS with sepsis. Diagnostic accuracy of indexes was equal to diagnostic accuracy of MFI for CD64 on neutrophils (0.833 versus 0.854 for day 0 and 0.975 versus 0.983 for day 1) and monocytes (0.811 versus 0.865 for day 0 and 0.825 versus 0.858 for day 1), and CD163 on neutrophils (0.595 versus 0.655 for day 0 and 0.677 versus 0.750 for day 1), but not for CD163 on monocytes.Conclusion. CD64 MFI, CD163 MFI, CD64 indexes for neutrophils and monocytes, and CD163 index for neutrophils can all be used for discrimination of SIRS and sepsis in critically ill neonates and children. CD64 index for neutrophils, however, is superior to all other markers.

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Diagnostic accuracy of sTREM-1 to identify infection in critically ill patients with systemic inflammatory response syndrome

Clinical Biochemistry ◽

10.1016/j.clinbiochem.2010.03.001 ◽

2010 ◽

Vol 43 (9) ◽

pp. 720-724 ◽

Cited By ~ 31

Author(s):

Jaime Latour-Pérez ◽

Adoración Alcalá-López ◽

Miguel-Ángel García-García ◽

José F. Sánchez-Hernández ◽

Carmen Abad-Terrado ◽

...

Keyword(s):

Systemic Inflammatory Response Syndrome ◽

Inflammatory Response ◽

Diagnostic Accuracy ◽

Critically Ill ◽

Critically Ill Patients ◽

Systemic Inflammatory Response

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Epidemiology of Blood Culture Utilization in a Cohort of Critically Ill Children

Journal of Pediatric Intensive Care ◽

10.1055/s-0038-1676993 ◽

2019 ◽

Vol 08 (03) ◽

pp. 144-147

Author(s):

Christine Anh-Thu Tran ◽

Jenna Verena Zschaebitz ◽

Michael Campbell Spaeder

Keyword(s):

Decision Making ◽

Blood Culture ◽

Systemic Inflammatory Response Syndrome ◽

Inflammatory Response ◽

Critically Ill ◽

Clinical Status ◽

Blood Cultures ◽

Systemic Inflammatory Response ◽

Critically Ill Children ◽

Culture Acquisition

AbstractBlood culture acquisition is integral in the assessment of patients with sepsis, though there exists a lack of clarity relating to clinical states that warrant acquisition. We investigated the clinical status of critically ill children in the timeframe proximate to acquisition of blood cultures. The associated rates of systemic inflammatory response syndrome (72%) and sepsis (57%) with blood culture acquisition were relatively low suggesting a potential overutilization of blood cultures. Efforts are needed to improve decision making at the time that acquisition of blood cultures is under consideration and promote percutaneous blood draws over indwelling lines.

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ADAMTS-13 in Critically Ill Patients With Septic Syndromes and Noninfectious Systemic Inflammatory Response Syndrome

Shock ◽

10.1097/shk.0000000000000341 ◽

2015 ◽

Vol 43 (6) ◽

pp. 556-562 ◽

Cited By ~ 19

Author(s):

Jesús Aibar ◽

Pedro Castro ◽

Gerard Espinosa ◽

Sara Fernández ◽

Cristina Hernández ◽

...

Keyword(s):

Systemic Inflammatory Response Syndrome ◽

Inflammatory Response ◽

Critically Ill ◽

Critically Ill Patients ◽

Systemic Inflammatory Response

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Procalcitonin and Interleukin-6 for predicting culturenegative and culture positive bacterial sepsis in critically ill patients with systemic inflammatory response syndrome

IIUM Medical Journal Malaysia ◽

10.31436/imjm.v15i1.1353 ◽

2020 ◽

Vol 15 (1) ◽

Author(s):

Mohd Basri Mat Nor ◽

Azrina Md Ralib

Keyword(s):

Systemic Inflammatory Response Syndrome ◽

Inflammatory Response ◽

Critically Ill ◽

Critically Ill Patients ◽

Interleukin 6 ◽

Systemic Inflammatory Response ◽

Bacterial Sepsis ◽

Saps Ii Score ◽

Culture Positive ◽

Culture Negative

Introduction: Differentiation between culture-negative bacterial sepsis (BS), culturepositive BS and non-infectious systemic inflammatory response syndrome (SIRS) among critically ill patients remains a diagnostic challenge to the intensive care unit (ICU) physicians. This study aimed to evaluate the role of procalcitonin (PCT) and interleukin-6 (IL-6) in predicting non-infectious SIRS, culture-negative BS and culture-positive BS in the ICU. Methods: This prospective observational study was conducted in a tertiary ICU in Pahang. The patients were divided into sepsis and non-infectious SIRS based on clinical assessment with or without positive cultures. Patients with positive cultures were further divided into bacteraemia and positive other culture. The PCT and IL-6 were measured daily over the first 3 days. Results: Two hundred and thirty nine consecutive patients diagnosed with SIRS were recruited, of whom 164 (69%) had sepsis. Among sepsis patients, there were 62 (37.8%) culture positive and 102 (62.2%) culture negative. Of these, 27 (16.5%) develop bacteraemia. The most common site of infection was respiratory (34.4%). Post-LSD analyses showed significant difference in the PCT between culture negative sepsis and SIRS (p=0.01); and positive other culture and SIRS (p=0.04). On the other hand IL-6 cannot differentiate between SIRS and negative culture sepsis (p=0.06). Both PCT and IL-6 predicted bacteraemia with an AUC of 0.70 (0.57 to 0.82) and 0.68 (0.53 to 0.70). IL-6 is independently associated with bacteraemia and other culture after adjusting for age, sex, hypertension, SAPS II score and day 1 PCT. Conclusions: Procalcitonin but not Interleukin-6 is able to differentiate SIRS from culture-negative BS. However, IL-6 is independently associated with bacteraemia and other culture.

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