scholarly journals Reaction of the Anticancer Organometallic Ruthenium Compound, [(η6-p-Cymene)Ru(ATSC)Cl]PF6 with Human Serum Albumin

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Floyd A. Beckford
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Thermodynamic Analysis ◽  
Binding Constant ◽  
Enthalpy Of Reaction ◽  
Infrared Spectrophotometry ◽  
Ruthenium Compound ◽  
Different Temperatures ◽  
Entropy Of Reaction

The reaction of [(η6-p-cymene)Ru(ATSC)Cl]PF6 (ATSC =9-anthraldehyde thiosemicarbazone) with human serum albumin was investigated at different temperatures using fluorescence and infrared spectrophotometry. The binding constant, K, for the reaction was determined using a number of different methods. Using a modified Stern-Volmer equation, K was determined to be 9.09×104,12.1×104, and 13.1×104 M−1 at 293 K, 298 K, and 308 K, respectively. A thermodynamic analysis showed that the reaction is spontaneous with ΔG being negative. The enthalpy of reaction ΔH=16.5 kJ mol−1 and the entropy of reaction ΔS=152 Jmol−1K−1. The values of ΔH and ΔS suggest that hydrophobic forces are dominant in the mode of interaction and that the process is mostly entropy driven.

scholarly journals Interaction of Warfarin with Human Serum Albumin and Effect of Ferulic Acid on the Binding

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Qiang Li ◽  
Wen-yue Yang ◽  
Ling-ling Qu ◽  
Huan-Yang Qi ◽  
Yun Huang ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Ferulic Acid ◽  
Human Serum ◽  
Binding Constant ◽  
Oral Anticoagulant ◽  
Combined Treatment ◽  
Hill Coefficient ◽  
Free Concentration ◽  
Risk Of Bleeding

Angelica sinensis(Oliv.) Diels combined treatment with warfarin would increase the risk of bleeding. Ferulic acid is an abundant hydroxycinnamic acid inA. sinensisand warfarin is the most widely used oral anticoagulant. The studies on supermolecular interaction of warfarin with human serum albumin (HSA) and the influence of ferulic acid on the binding would contribute to the understanding of the metabolic processes of warfarin and the effect of ferulic acid. We focus on investigating the effect of warfarin on fluorescence spectrum of human serum albumin (HSA), fluorescence quenching mechanism, binding constant, Hill coefficient, binding mode, and the effect of different ferulic acid concentrations on the binding. Warfarin quenched the intrinsic fluorescence of HSA mainly by static quenching. Accession of ferulic acid reduced the binding of HSA-warfarin. By decreasing binding constant and the Hill coefficient of warfarin with HSA, ferulic acid could improve the plasma concentration of free warfarin, which would increase the risk of bleeding. Warfarin’s free concentration increased at least 50% under the condition of simulated human body. The results indicated thatA. sinensiscombined treatment with warfarin would increase the risk of bleeding. And the results provide an important theoretical support for warfarin used as oral anticoagulant.

Biochemical, biophysical, and thermodynamic analysis of in vitro glycated human serum albumin

2007 ◽  
Vol 72 (2) ◽  
pp. 146-152 ◽  
Author(s):  
Mohd. Wajid Ali Khan ◽  
Zafar Rasheed ◽  
Wahid Ali Khan ◽  
Rashid Ali
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Thermodynamic Analysis
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