scholarly journals H-Point Standard Addition Method for Simultaneous Determination of Eosin and Erytrosine

2011 ◽  
Vol 8 (4) ◽  
pp. 1979-1985 ◽  
Author(s):  
Amandeep Kaur ◽  
Usha Gupta

A new, simple, sensitive and selectiveH-point standard addition method (HPSAM) has been developed for resolving binary mixture of food colorants eosin and erythrosine, which show overlapped spectra. The method is based on the complexation of food dyes eosin and erythrosine with Fe(III) complexing reagent at pH 5.5 and solubilizing complexes in triton x-100 micellar media. Absorbances at the two pairs of wavelengths, 540 and 550 nm (when eosin acts as analyte) or 518 and 542 nm (when erythrosine act as analyte) were monitored. This method has satisfactorily been applied for the determination of eosin and erythrosine dyes in synthetic mixtures and commercial products.

2012 ◽  
Vol 9 (1) ◽  
pp. 224-232
Author(s):  
Amandeep Kaur ◽  
Usha Gupta

H-point standard addition method (HPSAM) is developed for simultaneous determination of eosin-Yand ponceau-s in micellar media. Nickel chloride (NiCl2) is used as chromogenic reagent for complexes formation of eosin-Yand ponceau-Sfood colorants. The measurements were carried out using sodium lauryl sulphate as a surfactant, in buffered solution at pH 6.0. The concentration range of 0.115-2.53 μg/mL of eosin-Yand 0.159-3.80 μg/mL of ponceau-S. The proposed procedures have been applied successfully for the simultaneous determination of eosin-Yand ponceau-Sin synthetic binary mixtures and real samples.


2009 ◽  
Vol 6 (1) ◽  
pp. 106-112 ◽  
Author(s):  
P. P. Kaur ◽  
U. Gupta

The H-point standard addition method (HPSAM) has been applied for simultaneous determination of maneb and zineb using 1-(2-pyridylazo)-2- naphthol (PAN) as a reagent. Both maneb and zineb form wine red colored complex with PAN at pH 9.2 which are soluble in Triton X-100. Zineb and maneb can be determined simultaneously in the range of 0.414-3.446 µg mL-1and 0.663-3.316 µg mL-1respectively with satisfactory accuracy and precision. The proposed method has successfully been applied for the simultaneous determination of maneb and zineb in several synthetic samples, spiked water samples and crops


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