scholarly journals Spectrophotmetric Determination of Cefadroxil in Bulk and Dosage Form Using Sodium Hydroxide

2011 ◽  
Vol 8 (3) ◽  
pp. 1314-1322 ◽  
Author(s):  
S. W. Shantier ◽  
E. A. Gadkariem ◽  
K. E. Ibrahim ◽  
H. A. El-Obeid
Keyword(s):  
Sodium Hydroxide ◽  
Dosage Form ◽  
Molar Absorptivity ◽  
Direct Reaction ◽  
Confidence Limits ◽  
Chromatography Method ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatography Method ◽  
Liquid Chromatographic

A simple spectrophotometric method was developed for the determination of cefadroxil in pure bulk and in capsules forms. The method is based on a direct reaction between cefadroxil and sodium hydroxide (1 N). A product with λmaxat 342 nm and molar absorptivity of 7.9x103L mol-1cm-1is formed after heating cefadroxil with sodium hydroxide (1 N) for 30 minutes. The absorbance-concentration plot was rectilinear over the range 5-25 μg/mL with correlation coefficient values not less than 0.999. The detection limit (LOD) and quantification limit (LOQ) were 0.693 μg/mL and 2.31 μg/mL. The method was validated using the BP liquid chromatographic method for cefadroxil assay. The results obtained by the developed method for the capsules dosage form were statistically compared with those of the BP liquid chromatography method and evaluated at 95% confidence limits.

scholarly journals Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma

2013 ◽  
Vol 9 (2) ◽  
pp. 26-29 ◽  
Author(s):  
AK Hemanth Kumar ◽  
V Sudha ◽  
Geetha Ramachandran
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Pharmacokinetic Studies ◽  
Chromatography Method ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatography Method ◽  
Liquid Chromatographic

A high performance liquid chromatographic method for determination of rifabutin in human plasma was  developed. The method involved deproteinisation of the sample with acetonitrile and analysis of the  supernatant using a reversed-phase C18 column (250mm) and UV detection at a wavelength of 265nm.  The assay was specific for rifabutin and linear from 0.025 to 10.0μg/ml. The relative standard deviation  of intra- and inter-day assays was lower than 10%. The method was able to remove interfering materials  in plasma, yielding an average recovery of rifabutin from plasma of 101%. Due to its simplicity, the assay  can be used for pharmacokinetic studies of rifabutin. SAARC Journal of Tuberculosis, Lung Diseases & HIV/AIDS; 2012; IX(2) 26-29 DOI: http://dx.doi.org/10.3126/saarctb.v9i2.7975

A Green Liquid Chromatographic Method For The Simultaneous Determination of Chlorpheniramine Maleate, Pseudoephedrine Hydrochloride and Propyphenazone

2019 ◽  
Vol 15 (6) ◽  
pp. 635-641
Author(s):  
Nadia M. Mostafa ◽  
Ghada M. Elsayed ◽  
Nagiba Y. Hassan ◽  
Dina A. El Mously
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Dosage Form ◽  
Chromatographic Method ◽  
Green Analytical Chemistry ◽  
Chlorpheniramine Maleate ◽  
Accuracy And Precision ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Background:The concept of green analytical chemistry prevails due to the growing environmental pollution.Objective:Our attempts are to develop simple and eco-friendly method which is non-harmful to the environment by producing minimal waste. In this context, a green liquid chromatographic method was applied for the simultaneous determination of chlorpheniramine maleate, pseudoephedrine hydrochloride and propyphenazone in their combined dosage form.Methods:Separation was carried out using X select HSS RP C18 analytical column (250 × 4.6 mm, 5μm) using methanol - 0.02 M phosphate buffer pH 3 - triethylamine (60:40: 0.1, by volume) as a mobile phase. The separated peaks were detected at 215 nm at a flow rate 1.0 mL/min.Results:Quantification was done over the concentration ranges of 1-25 µg/mL for chlorpheniramine maleate, 5-35 µg/mL for pseudoephedrine hydrochloride and 10-120 µg/mL for propyphenazone. The suggested method was validated with regard to linearity, accuracy and precision according to the International Conference on Harmonization guidelines with good results.Conclusion:It could be used as a safer alternative for routine analysis of the mentioned drugs in quality control laboratories.

scholarly journals A modified high performance liquid chromatographic method for simultaneous quantification of skatole and indole in porcine plasma

2012 ◽  
Vol 81 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Carl Brunius ◽  
Galia Zamaratskaia
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
High Selectivity ◽  
High Performance Liquid Chromatographic ◽  
Boar Taint ◽  
Chromatography Method ◽  
Accuracy And Precision ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatography Method ◽  
Liquid Chromatographic

A simplified high-performance liquid chromatography method to measure skatole and indole in porcine plasma without the use of acetonitrile was developed and validated in this study. The mobile phase consisted of water and methanol used in a gradient programme. Fluorescence detection was performed on the supernatant obtained from plasma after protein precipitation with 100% acetone. Limits of quantification were 0.5 ng∙ml-1 for skatole and 1.0 ng∙ml-1 for indole. Accuracy and precision had less than 12% deviation in the linear ranges (0.5–256 ng∙ml-1 and R2 = 0.9999 for skatole, 1.0–256 ng∙ml-1 and R2 = 0.9999 for indole). The correlation between plasma and serum concentrations was strong for skatole (slope = 1.01, R2 = 0.999) and moderate for indole (slope = 0.65, R2 = 0.95). Analysis of skatole in plasma was in good accordance with our previous acetonitrile-based method (slope = 0.91, R2 = 0.988). The proposed method is suited for rapid routine analysis because of its high selectivity, accuracy and precision. Furthermore, it needs only simple sample preparation and the use of methanol instead of acetonitrile in the mobile phase. This method is of practical use to researchers in the field of boar taint.

RAPID HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF RALTEGRAVIR IN HUMAN SALIVA

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (09) ◽  
pp. 68-73
Author(s):  
K Vijaya Sri ◽  
M. Shiva Kumar ◽  
M. A. Madhuri ◽  
Suresha K. ◽  
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
High Performance Liquid Chromatographic ◽  
Human Saliva ◽  
Hplc Method ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Liquid Chromatography Method ◽  
Liquid Chromatographic

In this study, a high-performance liquid chromatographic method (HPLC) was developed, validated and applied for the determination of raltegravir in biological sample like saliva. Liquid- liquid extraction was performed for isolation of the drug and elimination of saliva interferences. Samples of saliva was extracted with 50µL of ortho phosphoric acid and 3ml of methanol was added and spiked with raltegravir. The chromatographic separation was performed on Agilent Eclipse C18 (100 mm × 4.6 mm, 3.5µm) column, by using 80:20 v/v acetonitrile: water as a mobile phase under isocratic conditions at a flow rate of 1.0 mL/min for UV detection at 240 nm. Retention time of raltegravir was found to be 1.030 min. Linearity was found to be in the range of 25-1000 ng/mL with regression equation y = 13864x + 40495 and correlation coefficient 0.999. The low % RSD value indicates the method is accurate and precise. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.76 and 2.28 ng/mL, respectively. It can be concluded that this validated HPLC method is easy, precise, accurate, sensitive and selective for determination of raltegravir in saliva.

scholarly journals A Rapid Determination of Cinnarizine in Bulk and Pharmaceutical Dosage Form by LC

2010 ◽  
Vol 7 (3) ◽  
pp. 1080-1084 ◽  
Author(s):  
A. A. Heda ◽  
A. R. Sonawane ◽  
G. H. Naranje ◽  
P. K. Puranik
Keyword(s):  
Potassium Hydroxide ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Rapid Determination ◽  
High Pressure Liquid Chromatographic ◽  
Pharmaceutical Dosage Form ◽  
Liquid Chromatographic Method ◽  
Limit Of Quantitation ◽  
Liquid Chromatographic

A simple, selective, rapid and precise reverse phase high pressure liquid chromatographic method has been developed for the estimation of cinnarizine from pharmaceutical formulation. The method was developed using MICRA-NPS C18(length×OD×ID =33×8.0×6.0 mm, 1.5 μm) column with a mobile phase consisting of acetonitrile, triethylamine buffer (adjusted to pH 4.5 with 10% w/v potassium hydroxide) and tetrahydrofuran in the ratio 30:66:4 respectively, at a flow rate of 0.5 mL/min. Wavelength was fixed at 253 nm. The developed method was validated for linearity, accuracy, precision, limit of detection and limit of quantitation. The proposed method can be used for the routine estimation of cinnarizine in pharmaceutical dosage form.

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