RP-HPLC Estimation of Trospium Chloride in Tablet Dosage Forms

A rapid, sensitive and precise HPLC method was developed for the estimation of trospium chloride in pure and tablet dosage forms. Seperation of the drug was achieved on a reverse phase Azilent C18column using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 60:40v/v. The flow rate was 1 ml/min and the detection wave length 215 nm. The linearity was found in the range of 10-150 μg/ml with a correlation coefficient of 1.0000. The proposed method was validated for its sensitivity, linearity, accuracy and precision. This method was employed for routine quality control analysis of trospium chloride in tablet dosage forms.

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Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method

E-Journal of Chemistry ◽

10.1155/2012/292754 ◽

2012 ◽

Vol 9 (1) ◽

pp. 340-344 ◽

Cited By ~ 3

Author(s):

V. Bhaskara Raju ◽

A. Lakshmana Rao

Keyword(s):

Quality Control ◽

Mobile Phase ◽

Dosage Forms ◽

Hplc Method ◽

Control Analysis ◽

Reverse Phase ◽

Quality Control Analysis ◽

Tablet Dosage ◽

Routine Quality Control

An accurate and preciseHPLCmethod was developed for the determination of lisinopril. Separation of the drug was achieved on a reverse phase C8column using a mobile phase consisting of phosphate buffer and methanol in the ratio of 35:65v/v. The flow rate was 0.8 mL/min and the detection wavelength was 215 nm. The linearity was observed in the range of 20-60 μ g/mL with a correlation coefficient of 0.9992. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of lisinopril in tablet dosage forms.

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Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC Method

E-Journal of Chemistry ◽

10.1155/2010/473798 ◽

2010 ◽

Vol 7 (1) ◽

pp. 180-184 ◽

Cited By ~ 6

Author(s):

D. Anantha Kumar ◽

G. Srinivasa Rao ◽

J. V. L. N. Seshagiri Rao

Keyword(s):

Simultaneous Determination ◽

Internal Standard ◽

Dosage Forms ◽

Hplc Method ◽

Control Analysis ◽

Potassium Dihydrogen ◽

Quality Control Analysis ◽

Rp Hplc ◽

Tablet Dosage

A simple, accurate and reproducible RP-HPLC method has been developed for the simultaneous determination of lamivudine, zidovudine and abacavir in tablet dosage forms. Chromatography was carried out on a HiQ Sil C 18 V column using a mobile phase consisting of 0.01 M potassium dihydrogenortho-phosphate (pH 3.0) and methanol (55:45 v/v) at a flow rate of 0.8 mL/min. The detection was made at 272 nm and stavudine was used as the internal standard for this study. The retention times for lamivudine, abacavir and zidovudine were found to be 3.8, 6.3, 8.1 min. respectively. The calibration curves were linear over the range 5-250 μg/mL for both zidovudine and abacavir and 5-140 μg/mL for lamivudine. The proposed method was validated as per ICH and USP guidelines and it was found suitable for the routine quality control analysis of the drugs in tablet dosage forms.

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DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF EDOXABAN TOSYLATE IN TABLET DOSAGE FORMS

INDIAN DRUGS ◽

10.53879/id.57.07.11389 ◽

2020 ◽

Vol 57 (07) ◽

pp. 47-51

Author(s):

B. Anupama ◽

P. Tejaswi ◽

KNV. Chenchu Lakshmi ◽

A. Vishwanadh

Keyword(s):

High Performance ◽

Reversed Phase ◽

Dosage Forms ◽

Hplc Method ◽

Control Analysis ◽

Rp Hplc ◽

Ich Guidelines ◽

Development And Validation ◽

Tablet Dosage ◽

Isocratic Mode

A rapid, simple and precise reversed phase high performance liquid chromatography (RP-HPLC) method was developed for the estimation of edoxabantosylate in tablets. The quantification was carried out using a Phenomenex C-18 column (250×4.6 mm i.d., 5µm particle size) in isocratic mode with mobile phase comprising of ammonium acetate buffer andacetonitrile in the ratio of 50:50 (V/V) at a flow rate 1 mL/min. The eluent was monitored at 240 nm. The retention time of the drug was 3.486 min. The calibration curve was linear in the concentration range of 5-25 µg/mL and per cent recovery ranged from 98.25-101.6.The developed method was validated as per ICH guidelines and the results obtained were satisfactory.The method can be applied for routine quality control analysis of edoxabantosylate in tablet dosage forms.

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Development and Validation of a Stability-Indicating Method for the Determination of Pazopanib Hydrochloride by RP-HPLC

International Journal of Pharmaceutical Sciences Review and Research ◽

10.47583/ijpsrr.2021.v68i01.035 ◽

2021 ◽

Vol 68 (1) ◽

Author(s):

Ravisankar P. ◽

M. Nithya Satya ◽

A. Bhavani Sailu ◽

Sk. Rijwana ◽

K. Harsha Sri ◽

...

Keyword(s):

Quality Control ◽

Thermal Conditions ◽

Hplc Method ◽

Control Analysis ◽

Pharmaceutical Tablets ◽

Stability Indicating Method ◽

Quality Control Analysis ◽

Rp Hplc ◽

Tablet Dosage ◽

Routine Quality Control

A precise, accurate, highly sensitive, rapid, and reproducible stability HPLC method was developed and validated for the estimation of Pazopanib Hydrochloride (PAZO) in bulk and tablet dosage form. Decent quality chromatographic separation of Pazopanib Hydrochloride was done by using Eclipse plus C18 column (4.5 mm i.e. X 150 mm, 3.5µm particle size) (based on 99.99 % ultra-high purity silica) using mobile phase that containing 0.1 % Orthophosphoric acid: Acetonitrile (55:45 % v\v) at a flow rate of 1.0 mL/minute. The wavelength ?max of PAZO used for the detection was found to be 271.4 nm. The retention time for Pazopanib was found to 1.43 minutes. The PAZO was linear in the concentration range of 2-10 µg/mL (r2 = 0.9999) for HPLC method. The regression equation for PAZO was found to be Y = 700955 x + 28022 for HPLC. The LOD and LOQ were found to be 0.1675 ?g/mL, 0.0552 µg/mL for the HPLC method, respectively. The developed methods are validated in pursuance of ICH Q2 (R1) guidelines. The method was linear, precise, accurate with recoveries in the range of 98 – 102 %, and minimum values of % RSD indicate the accuracy of the method. The % assay of the PAZO was found to be 99.85 ± 1.2, which was in good agreement with the labeled claim. Pazopanib was subjected to stressed conditions like acidic, basic, oxidative, photolytic, and thermal conditions. The degradation results were found satisfactory. The developed gradient RP-HPLC method can be successfully practiced for the routine quality control analysis of PAZO in pharmaceutical tablets and routine quality control analysis.

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A new stability-indicating RP-HPLC method for the determination of dicyclomine hydrochloride and dimethicone combination in tablet dosage forms

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-021-00260-0 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

J. Saroja ◽

Anantha Lakshmi P.V. ◽

Y. Rammohan ◽

D. Divya Reddy

Keyword(s):

Liquid Chromatography ◽

Dosage Forms ◽

Flow Speed ◽

Hplc Method ◽

Stability Indicating ◽

Simultaneous Quantification ◽

Rp Hplc ◽

Tablet Formulations ◽

Tablet Dosage

Abstract Background We describe a “stability-indicating liquid chromatography” technique for the estimation of dimethicone (DEC) and dicyclomine hydrochloride (DEH) in the established tablet formulations. Individual quantification of DEH and DEC was reported. But simultaneous quantification of DEH and DEC was lacking. DEH and DEC were analysed on an “XTerra C18 column (250 mm × 4.6 mm, 5 μm)” with the mobile phase solvent run isocratically with 0.1M K2HPO4-acetonitrile (55:45, v/v) on a flow speed of 1.0 mL/min. Results The chromatographic run period for the DEC and DEH assay was 6.0 min with retention times of 2.134 and 2.865 min, respectively. The method was validated for accuracy (99.453 to 100.417% and 99.703 to 100.303% recovery values for DEH and DEC, respectively), precision (RSV value 0.135% for DEC and 0.171% for DEH), linearity (5–15 μg/mL for DEH and 20–60 μg/mL for DEC), selectivity (no hinderance from excipients) and specificity (no hinderance from degradants) recovery. Conclusion The developed stability-indicating liquid chromatography process was well applied to established tablet formulations.

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RP-HPLC Determination of Raloxifene in Pharmaceuticl Tablets

E-Journal of Chemistry ◽

10.1155/2006/713569 ◽

2006 ◽

Vol 3 (1) ◽

pp. 60-64 ◽

Cited By ~ 6

Author(s):

P. Venkata Reddy ◽

B. Sudha Rani ◽

G. Srinu Babu ◽

J. V. L. N. Seshagiri Rao

Keyword(s):

Flow Rate ◽

Retention Time ◽

Mobile Phase ◽

Dosage Forms ◽

Hplc Method ◽

Reverse Phase Hplc ◽

Reverse Phase ◽

Pharmaceutical Dosage Forms ◽

Rp Hplc

A reverse phase HPLC method is developed for the determination of Raloxifene in pharmaceutical dosage forms. Chromatography was carried out on an inertsil C18 column using a mixture of acetonitrile and phosphate buffer (30:70 v/v) as the mobile phase at a flow rate of 1 mL/min. Detection was carried out at 290 nm .The retention time of the drug was 10.609 min. The method produced linear responses in the concentration range of 0.5-200 µg/mL of Raloxifene. The method was found to be applicable for determination of the drug in tablets.

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