scholarly journals Histology and Ultrastructure of Transitional Changes in Skin Morphology in the Juvenile and Adult Four-Striped Mouse (Rhabdomys pumilio)

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Eranée Stewart ◽  
Moyosore Salihu Ajao ◽  
Amadi Ogonda Ihunwo
Keyword(s):  
Adult Mouse ◽  
Mouse Skin ◽  
Hair Follicles ◽  
Ultrastructural Analysis ◽  
Rhabdomys Pumilio ◽  
Mammalian Skin ◽  
Skin Morphology ◽  
Masson Trichrome Staining

The four-striped mouse has a grey to brown coloured coat with four characteristic dark stripes interspersed with three lighter stripes running along its back. The histological differences in the skin of the juvenile and adult mouse were investigated by Haematoxylin and Eosin and Masson Trichrome staining, while melanocytes in the skin were studied through melanin-specific Ferro-ferricyanide staining. The ultrastructure of the juvenile skin, hair follicles, and melanocytes was also explored. In both the juvenile and adult four-striped mouse, pigment-containing cells were observed in the dermis and were homogeneously dispersed throughout this layer. Apart from these cells, the histology of the skin of the adult four-striped mouse was similar to normal mammalian skin. In the juvenile four-striped mouse, abundant hair follicles of varying sizes were observed in the dermis and hypodermis, while hair follicles of similar size were only present in the dermis of adult four-striped mouse. Ultrastructural analysis of juvenile hair follicles revealed that the arrangement and differentiation of cellular layers were typical of a mammal. This study therefore provides unique transition pattern in the four-striped mouse skin morphology different from the textbook description of the normal mammalian skin.

scholarly journals NG2 Proteoglycan Expression in Mouse Skin: Altered Postnatal Skin Development in the NG2 Null Mouse

2007 ◽  
Vol 56 (3) ◽  
pp. 295-303 ◽  
Author(s):  
Kuniko Kadoya ◽  
Jun-ichi Fukushi ◽  
Yoshihiro Matsumoto ◽  
Yu Yamaguchi ◽  
William B. Stallcup
Keyword(s):  
Stem Cell ◽  
Mouse Skin ◽  
Hair Follicles ◽  
Stem Cell Population ◽  
Null Mouse ◽  
Outer Root Sheath ◽  
Skin Development ◽  
Bulge Region ◽  
Ng2 Proteoglycan ◽  
Stem Cell Populations

In early postnatal mouse skin, the NG2 proteoglycan is expressed in the subcutis, the dermis, the outer root sheath of hair follicles, and the basal keratinocyte layer of the epidermis. With further development, NG2 is most prominently expressed by stem cells in the hair follicle bulge region, as also observed in adult human skin. During telogen and anagen phases of the adult hair cycle, NG2 is also found in stem cell populations that reside in dermal papillae and the outer root sheaths of hair follicles. Ablation of NG2 produces alterations in both the epidermis and subcutis layers of neonatal skin. Compared with wild type, the NG2 null epidermis does not achieve its full thickness due to reduced proliferation of basal keratinocytes that serve as the stem cell population in this layer. Thickening of the subcutis is also delayed in NG2 null skin due to deficiencies in the adipocyte population.

scholarly journals Suprabasal Induction of Ornithine Decarboxylase in Adult Mouse Skin Is Sufficient to Activate Keratinocytes

2005 ◽  
Vol 124 (3) ◽  
pp. 602-614 ◽  
Author(s):  
Li Lan ◽  
Candace S. Hayes ◽  
Lisa Laury-Kleintop ◽  
Susan K. Gilmour
Keyword(s):  
Ornithine Decarboxylase ◽  
Adult Mouse ◽  
Mouse Skin

EFFECTS OF STEROID HORMONES ON DEVELOPING MOUSE SKIN IN VITRO

1975 ◽  
Vol 66 (2) ◽  
pp. 195-205 ◽  
Author(s):  
AMREEK SINGH ◽  
MARGARET H. HARDY
Keyword(s):  
Cell Differentiation ◽  
Organ Culture ◽  
Sebaceous Gland ◽  
Mouse Skin ◽  
Hair Follicles ◽  
Stratum Granulosum ◽  
Normal Medium ◽  
Hormonal Steroids ◽  
Good Differentiation

SUMMARY Pieces of skin from 13·5- to 15-day-old foetal mice were grown in organ culture in a biological medium with or without the addition of hormonal steroids. Cortisol (7·5 μg/ml) caused thinning of the non-cornified epidermis and flattening of the stratum granulosum after 3 days. By 6 days the epidermis was thinner and hair follicles were regressing, and these changes continued up to 12 days. Administration of corticosterone (5 μg/ml) also produced thinning of the epidermis and regression of the follicles after 6 days. Good differentiation of epidermis and hair follicles was obtained when testosterone (100 μg/ml) was added to the medium. The non-cornified epidermal layers were similar to those of control cultures at 3 days but less than half as thick at 6 days. Hair follicles differentiated as rapidly in medium containing testosterone as in normal medium, but, unlike in the latter medium, also developed sebaceous gland anlagen at 6 days. Some explants in testosterone medium showed signs of sebaceous cell differentiation at 9 days.

Recognition and invasion of human skin by Schistosoma mansoni cercariae: the key-role of L-arginine

Parasitology ◽  
2002 ◽  
Vol 124 (2) ◽  
pp. 153-167 ◽  
Author(s):  
W. HAAS ◽  
K. GRABE ◽  
C. GEIS ◽  
T. PÄCH ◽  
K. STOLL ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Mouse Skin ◽  
Ventral Side ◽  
Body Axis ◽  
Surface Layers ◽  
Mammalian Skin ◽  
Cellular Immune ◽  
Arginine Concentration

The attachment of Schistosoma mansoni cercariae to mammalian skin is specifically stimulated by L-arginine. As L-arginine is an unsuitable signal for a specific identification of mammalian skin we examined the following 5 hypotheses to explain the advantage of the cercarial sensitivity to L-arginine. (1) A Schistosoma infection lowered the arginine concentration in the serum of mice, and this could enable the cercariae to avoid attachments to already infected mice. However, the infection did not reduce the arginine concentration in the skin and the cercarial attachment responses to it. (2) Creeping cercariae showed chemotactic orientation specifically along increasing L-arginine gradients. L-arginine could act as a pheromone which could guide cercariae towards common penetration sites. However, the cercarial acetabular gland contents were not attractive and they did not (in contrast to previous reports) contain much arginine. (3) Schistosomula (transformed cercariae) could use L-arginine to produce nitric oxide (NO) for blood vessel dilation during their migration in the host. However, in vitro the transformed cercariae did not convert L-arginine into citrulline and NO. (4) Schistosomula could bind L-arginine from the surrounding tissues and so escape the cellular immune attack (which needs L-arginine as the precursor of NO). However, transformed cercariae bound no more L-arginine than L-serine and L-lysine. (5) Schistosomula, migrating parallel to the surface in the mammalian epidermis, are dependent on information on their position between the inner and the surface layers of the skin. In the mouse skin, they adjusted their body axis with the ventral side toward the deeper (arginine-residue rich) epidermis layers. When migrating in agar, they showed chemo-orientation toward serum, and D-glucose and L-arginine were the stimulating compounds therein. The burrowing schistosomula adjusted their body axis (as in the epidermis) with the ventral side toward the higher concentration of L-arginine and not of glucose. We argue that the sensitivity for L-arginine has its primary function in orientation within mammalian skin and in location of blood vessels.

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