Validation of Antimycobacterial Plants Used by Traditional Healers in Three Districts of the Limpopo Province (South Africa)
The aim of the study was to scientifically evaluate the antimycobacterial activity of selected indigenous medicinal plants from the Limpopo Province used for the treatment of humans with symptoms ofMycobacterium tuberculosis. The leaves of five plant species (Apodytes dimidiata, Artemisia, Combretum hereroense, Lippia javanica, and Zanthoxylum capense) were collected from the Lowveld National Botanical Garden in Nelspruit, South Africa. The dried leaves were powdered and extracted using hexane, dichloromethane, acetone, and methanol. Antimycobacterial activity was evaluated using microdilution assay and bioautography andρ-iodonitrotetrazolium violet (INT) as indicator. Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH). Phytochemical content of extracts was further evaluated. The acetone extracts ofL. javanicadisplayed antioxidant activity on BEA chromatogram. T Acetone extracts ofA. afrahad MIC value of 0.39 mg/mL againstMycobacterium smegmatisATCC 1441. Acetone extracts ofC. hereroenseandL. javanicahad MIC value of 0.47 mg/mL. Four bands that inhibited the growth ofM. smegmatiswere observed atRfvalues of 0.12, 0.63, and 0.87 on BEA and 0.73 on EMW. The plant speciesA. dimidiata, A. afra, C. hereroense,andL. javanicain this study demonstrated their potential as sources of anti-TB drug leads.