scholarly journals Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Yu-Hoon Kim ◽  
Sang Eon Shin ◽  
Chan Seon Ham ◽  
Seong Yoon Kim ◽  
Kwang Soo Ko ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Sequence Comparison ◽  
Forensic Entomology ◽  
Pcr Amplification ◽  
Distance Matrix ◽  
Nucleotide Sequences ◽  
Species Level ◽  
Coi Gene ◽  
Oxidase Subunit

Identification of insect species is an important task in forensic entomology. For more convenient species identification, the nucleotide sequences of cytochrome c oxidase subunit I (COI) gene have been widely utilized. We analyzed full-length COI nucleotide sequences of 10 Muscidae and 6 Sarcophagidae fly species collected in Korea. After DNA extraction from collected flies, PCR amplification and automatic sequencing of the whole COI sequence were performed. Obtained sequences were analyzed for a phylogenetic tree and a distance matrix. Our data showed very low intraspecific sequence distances and species-level monophylies. However, sequence comparison with previously reported sequences revealed a few inconsistencies or paraphylies requiring further investigation. To the best of our knowledge, this study is the first report ofCOInucleotide sequences fromHydrotaea occulta, Muscina angustifrons, Muscina pascuorum, Ophyra leucostoma, Sarcophaga haemorrhoidalis, Sarcophaga harpax, andPhaonia aureola.

scholarly journals DNA barcoding of Crustacean larvae in the eastern areas of Segara Anakan Cilacap, Central Java Indonesia

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
KUSBIYANTO KUSBIYANTO ◽  
DIAN BHAGAWATI ◽  
Agus Nuryanto
Keyword(s):  
Cytochrome C ◽  
Genetic Distance ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Species Level ◽  
Coi Gene ◽  
Reference Species ◽  
Oxidase Gene ◽  
Oxidase Subunit ◽  
Central Java

Abstract. Kusbiyanto, Bhagawati D, Nuryanto A. 2020. DNA barcoding of crustacean larvae in Segara Anakan, Cilacap, Central Java, Indonesia using cytochrome c oxidase gene. Biodiversitas 21: 4878-4887. Species-level identification of crustacean larvae is challenging due to morphological constraints. DNA barcoding offers a precise method to solve the problems. That method has never been applied to crustacean larvae from the eastern of Segara Anakan, Cilacap, Central Java, Indonesia. This study aims to identify crustacean larvae in the eastern of Segara Anakan using the cytochrome c oxidase subunit I (COI) gene as a barcode marker. Larvae morphotypes were identified under a binocular microscope. The COI gene was sequenced from one individual of each morphotype. Microscopic observation placed the samples into 15 morphotypes. DNA barcoding placed twelve morphotypes as Crustacea with sequence homologies from 72.21% to 99.21%. Intra-species genetic divergences between samples and reference species ranged between 0.9% and 31.9%, while genetic distance ranged from 0.0% to 17.80%. Intra-species genetic divergences ranged between 0.00% and 3.9%, while genetic distance ranged from 0.00% to 3.8%. The phylogenetic tree proved the monophyly between samples and reference species and showed clear separation among species. All parameters proved that nine morphotypes were identified into species level and were counted for five species. Three morphotypes were identified into the genus level and were counted for three genera. Eight species of crustacean larvae were successfully identified using the cytochrome c oxidase subunit 1 gene.

scholarly journals Molecular identification of Sarcocystis spp. in cattle: partial sequencing of Cytochrome C Oxidase subunit 1 (COI)

2019 ◽  
Vol 7 (4) ◽  
Author(s):  
Selene Rubiola ◽  
Francesco Chiesa ◽  
Stefania Zanet ◽  
Tiziana Civera
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Molecular Techniques ◽  
Coi Gene ◽  
Rrna Gene ◽  
Intermediate Hosts ◽  
Bovine Muscle ◽  
Oxidase Subunit ◽  
Sarcocystis Spp

Sarcocystis spp. are protozoan parasites with an obligatory two-host life cycle, with herbivores as intermediate hosts and carnivores as definitive hosts. Cattle are intermediate hosts for several species of Sarcocystis: indeed, in addition to S. cruzi, S. hirsuta and S. hominis, at least four new species were recently identified in bovine muscle: S. bovifelis, S. rommeli, S. bovini and S. heydorni. Since is not possible to unambiguously discriminate between S. hominis and the new species either morphologically or by the analysis of the 18S ribosomial (rRNA) gene, the aim of the present study was to use molecular techniques to discriminate cattle Sarcocystis species, taking advantage of the higher discriminative power of the Cytochrome C Oxidase subunit I mitochondrial (mtDNA COI) gene. Therefore, 119 bovine muscle samples were tested to identify S. hominis-like sarcocystis using a multiplex PCR of the 18S rRNA gene; later, positive samples were tested using a newly designed primer set for the PCR amplification of COI gene. Species identification was achieved by sequencing the amplified products: 16 sequences were confirmed to belong to S. bovifelis, while 12 sequences didn’t constitute the best BLAST match of any of the published sequences, allowing to speculate the possible presence of S. hominis. This study confirms the higher discriminatory power of COI mitochondrial gene; besides, our work provides the first report of S. bovifelis in Italy.

scholarly journals Culturing embryonic cells from the parthenogenetic clonal marble crayfish (Marmorkrebs) Procambarus virginalis Lyko, 2017 (Decapoda: Astacidea: Cambaridae)

2019 ◽  
Vol 39 (6) ◽  
pp. 758-763
Author(s):  
Heriberto Deleon ◽  
Juan Garcia ◽  
Dionn Carlo Silva ◽  
Oscar Quintanilla ◽  
Zen Faulkes ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Early Stage ◽  
Model Organism ◽  
Coi Gene ◽  
Embryonic Cells ◽  
Oxidase Subunit ◽  
Marbled Crayfish

Abstract The parthenogenetic marbled crayfish, or Marmorkrebs (Procambarus virginalis Lyko 2017), is an emerging model organism. We describe a method to isolate cells from early-stage embryos and culture them in vitro. The identity of the cells was confirmed by sequencing the cytochrome c oxidase subunit I (COI) gene. This technique can be applied for use in the manipulation of embryonic parthenogenetic crayfish cells.

Two new feather mites (Acari: Analgoidea) isolated from the grey-headed woodpecker, Picus canus (Piciformes: Picidae) in Korea

2019 ◽  
Vol 24 (11) ◽  
pp. 2167-2183
Author(s):  
Yeong-deok Han ◽  
Sergey V. Mironov ◽  
Gi-sik Min
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Dna Barcodes ◽  
Feather Mites ◽  
Oxidase Subunit ◽  
Two New Species ◽  
First Time

Two new species of feather mites from the superfamily Analgoidea are described from the grey-headed woodpecker, Picus canus, in Korea: Neopteronyssus koreanus sp. nov. (Pteronyssidae) and Proterothrix picinus sp. nov. (Proctophyllodidae: Pterodectinae). Feather mites of the genera Neopteronyssus Mironov, 2002 and Proterothrix Gaud, 1968 are described for the first time in Korea. Morphological descriptions of both new species are complemented with partial sequences of their mitochondrial cytochrome c oxidase subunit I (COI) gene as DNA barcodes.

Comparative study on DNA sequences of ribosomal DNA and cytochrome c oxidase subunit 1 of mitochondrial DNA among five species of gnathostomes

2006 ◽  
Vol 80 (1) ◽  
pp. 7-13 ◽  
Author(s):  
K. Ando ◽  
M. Tsunemori ◽  
H. Akahane ◽  
S. Tesana ◽  
H. Hasegawa ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Nucleotide Sequences ◽  
Internal Transcribed Spacer Region ◽  
Cycle Sequencing ◽  
Oxidase Subunit ◽  
Species Being

AbstractThe nucleotide sequences of partial 18S, complete internal transcribed spacer region 1 (ITS1), complete 5.8S, complete ITS2 and partial 28S of ribosomal DNA (rDNA) and cytochrome c oxidase subunit 1 of mitochondrial DNA (MCOI) from five species of gnathostomes (G. spinigerum, G. doloresi, G. nipponicum, G. hispidum and G. binucleatum with the former four species being distributed in Japan and Asia) that cause human gnathostomiasis were compared by direct polymerase chain reaction cycle-sequencing. The nucleotide sequences of each region of the18S (613 bp), 5.8S (158 bp) and 28S (598 bp) rDNA from the five species were almost identical. The ITS1 region was different in length for the five species. The nucleotide sequences of each region of ITS2 and partial MCO1 regions were different among the five species. Therefore, these two regions can be used as genetic markers for identification of worms.

scholarly journals Molecular characterization of human isolates of Strongyloides stercoralis and Rhabditis spp. based on mitochondrial cytochrome c oxidase subunit 1 (cox1)

2019 ◽  
Author(s):  
Mandana Fadaei Tehrani ◽  
Meysam Sharifdini ◽  
Farzaneh Zahabiun ◽  
Robabeh Latifi ◽  
Eshrat bigom Kia
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Molecular Characterization ◽  
Mitochondrial Gene ◽  
Pcr Amplification ◽  
Strongyloides Stercoralis ◽  
Cox1 Gene ◽  
Oxidase Subunit ◽  
Stool Samples ◽  
Fresh Stool

Abstract Background: In microscopical examinations of stool samples, S. stercoralis may be miss-diagnosed with other nematodes infecting human digestive system, especially Rhabditis species that release larvae in stool samples. The aim of this study was molecular characterization and differentiation of human derived isolates of S. stercoralis and Rhabditis species based on the mitochondrial gene of cytochrome c oxidase subunit 1 (cox1) amplification. Methods: Using parasitological methods, ten isolates of S. stercoralis and three isolates of Rhabditis spp. were obtained from fresh stool samples of patients and the genomic DNA of the samples were extracted. PCR amplification of cox1 gene was carried out for all the isolates and the products were sequenced. Results: The phylogenetic analysis illustrated that S. stercoralis and Rhabditis spp. isolates were placed in two distinguishable separate clades. Inter-species genetic variation between isolates of S. stercoralis and Rhabditis spp. were ranged from 13.5 to 14.5%. Conclusions: Cox1 gene was a suitable marker for discrimination of S. stercoralis from Rhabditis spp. retrieved from human in the current study.

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