scholarly journals Complex Links between Natural Tuberculosis and Porcine Circovirus Type 2 Infection in Wild Boar

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Iratxe Díez-Delgado ◽  
Mariana Boadella ◽  
MariPaz Martín-Hernando ◽  
José Angel Barasona ◽  
Beatriz Beltrán-Beck ◽  
...  
Keyword(s):  
Wild Boar ◽  
Body Condition ◽  
Natural Infection ◽  
Natural Populations ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Frequent Event ◽  
Free Ranging

Individuals in natural populations are exposed to a diversity of pathogens which results in coinfections. The aim of this study was to investigate the relation between natural infection with tuberculosis (TB) due to infection by bacteria of theMycobacterium tuberculosiscomplex and porcine circovirus type 2 (PCV2) in free-ranging Eurasian wild boar (Sus scrofa). Apparent prevalence for TB lesions and PCV2 infection was extremely high in all age classes, including piglets (51% for TB; 85.7% for PCV2). Modeling results revealed that the relative risk of young (less than 2 years old) wild boar to test positive to PCV2 PCR was negatively associated with TB lesion presence. Also, an interaction between TB, PCV2, and body condition was evidenced: in wild boar with TB lesions probability of being PCV2 PCR positive increased with body condition, whereas this relation was negative for wild boar without TB lesions. This study provides insight into the coinfections occurring in free-ranging host populations that are naturally exposed to several pathogens at an early age. Using TB and PCV2 as a case study, we showed that coinfection is a frequent event among natural populations that takes place early in life with complex effects on the infections and the hosts.

Genetic characterization of porcine circovirus type 2 in the Korean wild boar population

2014 ◽  
Vol 169 (3-4) ◽  
pp. 147-153 ◽  
Author(s):  
Dong-Jun An ◽  
Seong-In Lim ◽  
Yong Kwan Kim ◽  
Hyun-Kyoung Lee ◽  
Yoon-Young Cho ◽  
...  
Keyword(s):  
Wild Boar ◽  
Genetic Characterization ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Wild Boar Population

scholarly journals Influence of Porcine Circovirus Type 2 Infections on Bovine Tuberculosis in Wild Boar Populations

2013 ◽  
Vol 60 ◽  
pp. 121-127 ◽  
Author(s):  
D. Risco ◽  
P. Fernández-Llario ◽  
W. L. García-Jiménez ◽  
P. Gonçalves ◽  
J. M. Cuesta ◽  
...  
Keyword(s):  
Wild Boar ◽  
Bovine Tuberculosis ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus

scholarly journals A double-antibody sandwich ELISA based on the porcine circovirus type 2 (PCV2) propagated in cell culture for antibody detection

2016 ◽  
Vol 36 (12) ◽  
pp. 1171-1177 ◽  
Author(s):  
Taís F. Cruz ◽  
Tatiana M. Kanashiro ◽  
Alessandra M.M.G. de Castro ◽  
Cintia M. Baldin ◽  
Leonardo J. Richtzenhain ◽  
...  
Keyword(s):  
Cell Culture ◽  
Natural Infection ◽  
Sandwich Elisa ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Antibody Detection ◽  
Porcine Circovirus ◽  
Sucrose Cushion ◽  
Double Antibody

ABSTRACT: Few studies have described enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against porcine circovirus type 2 (PCV2) based on antigens produced in cell culture. Furthermore, few articles have described viral purification techniques for members of the family Circoviridae. This occurs because circoviruses are difficult to isolate, noncytopathogenic, and produce low viral titres in cell culture. Thus, for overcoming these difficulties in the cultivation of PCV2, this study aimed to develop a double-antibody sandwich ELISA based on the cell culture antigen PCV2b for the quantification of anti-PCV2 antibodies. A 20% and 50% discontinuous sucrose cushion was used for viral purification, which enabled the separation of cell culture proteins in the 20% sucrose cushion and a greater viral concentration in the 50% sucrose cushion. Following isopycnic centrifugation, PCV2 was concentrated in the band with density values from 1.330 to 1.395g/cm3. Viral purification was assessed using SDS-PAGE, indirect ELISA and electron microscopy. The standardised ELISA revealed a strong linear correlation (r= 0.826, p<0.001) when compared with a commercial ELISA kit. The assay exhibited low variability (inter-assay coefficient of variation of 4.24% and intra-assay of 1.80%) and excellent analytical specificity conferred by the capture antibody produced in rabbit. Thus, this ELISA is a rapid, specific and convenient method for the detection of antibodies against PCV2 in studies of experimental and natural infection, and in monitoring the response to vaccination on commercial farms.

scholarly journals Antibody Recognition of Porcine Circovirus Type 2 Capsid Protein Epitopes after Vaccination, Infection, and Disease

2011 ◽  
Vol 18 (5) ◽  
pp. 749-757 ◽  
Author(s):  
Benjamin R. Trible ◽  
Maureen Kerrigan ◽  
Nicholas Crossland ◽  
Megan Potter ◽  
Kay Faaberg ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Natural Infection ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Reading Frame ◽  
Antibody Recognition ◽  
Wasting Syndrome ◽  
Study Support

ABSTRACTOpen reading frame 2 (ORF2) of porcine circovirus type 2 (PCV2) codes for the 233-amino-acid capsid protein (CP). Baculovirus-based vaccines that express only ORF2 are protective against clinical disease following experimental challenge or natural infection. The goal of this study was to identify regions in CP preferentially recognized by sera from experimentally infected and vaccinated pigs and to compare these responses to those of pigs diagnosed with porcine circovirus-associated disease (PCVAD), including porcine multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). The approach was to react porcine sera with CP polypeptide fragments followed by finer mapping studies using overlapping oligopeptides that covered amino acids 141 to 200. The results showed that vaccinated pigs preferentially recognized only the largest polypeptide fragment, CP(43-233). A subset of experimentally infected pigs and pigs with PDNS showed strong reactivity against a CP oligopeptide, 169-STIDYFQPNNKR-180. Alanine scanning identified Y-173, F-174, Q-175, and K-179 as important for antibody recognition. The results from this study support the notion of PCV2 modulation of immunity, including antibody responses that may represent a precursor for disease. The recognition of CP(169-180) and other polypeptides provides opportunities to devise diagnostic tests for monitoring the immunological effectiveness of vaccination.

scholarly journals Epidemiological study on porcine circovirus type 2 (PCV2) infection in the European wild boar (Sus scrofa)

2004 ◽  
Vol 35 (2) ◽  
pp. 243-253 ◽  
Author(s):  
Joaquin Vicente ◽  
Joaquim Segal�s ◽  
Ursula H�fle ◽  
M�nica Balasch ◽  
Joan Plana-Dur�n ◽  
...  
Keyword(s):  
Wild Boar ◽  
Epidemiological Study ◽  
Sus Scrofa ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Pcv2 Infection ◽  
Porcine Circovirus ◽  
European Wild Boar

scholarly journals Evidence for recombination in natural populations of porcine circovirus type 2 in Hong Kong and mainland China

2007 ◽  
Vol 88 (6) ◽  
pp. 1733-1737 ◽  
Author(s):  
Ching-Man Ma ◽  
Chung-Chau Hon ◽  
Tsan-Yuk Lam ◽  
Vince Yik-Yeung Li ◽  
Creany Ka-Wai Wong ◽  
...  
Keyword(s):  
Hong Kong ◽  
Phylogenetic Analyses ◽  
Statistical Significance ◽  
Preliminary Investigation ◽  
Natural Populations ◽  
Mainland China ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus

Porcine circovirus type 2 (PCV2) belongs to the family Circoviridae, and is the causative agent of post-weaning multisystemic wasting syndrome (PMWS) in pigs. In this study, phylogenetic analyses of three complete PCV2 genomic sequences from Hong Kong suggest that natural recombination happened among different lineages of PCV2. A preliminary investigation of the parental strains of these potential recombinants was carried out using bootscanning. Statistical significance of this recombination event was tested and positions of the potential recombination breakpoints were estimated in a maximum-likelihood framework. The recombinant breakpoints were estimated to be located within the origin of replication (ori) and replicase (rep) gene of PCV2. Interestingly, several GenBank sequences of PCV2 in mainland China were found to have a recombination pattern similar to that of the potential PCV2 recombinants from Hong Kong, implying that this recombinant genotype might already be widespread within mainland China.

Sign in / Sign up

Export Citation Format

Share Document