Study of Effect of Salvianolic Acid B on Motor Function Recovery in Rats with Spinal Cord Injury

In this study effect of salvianolic acid B was observed on motor function recovery of rats with spinal cord injury. 50 rats were selected and after inducing SCI their recovery under controlled conditions was studied using Sal B and PBS (as control). Both compounds were introduced intraperitoneally in respective groups of traumatic rats at the same time intervals for 28 days. It was observed that Sal B introduced at 5 mg/kg/day resulted in better motor function recovery. BBB score was recorded which increased significantly along with the reduction in cavity area observed by bright field microscopy of tissues, that is, from 1 to 10 and from0.20±0.05 mm2to0.10±0.03 mm2, in Sal B treated group, respectively, compared to PBS group. Statistical analysis was carried out using SPSS software (SPSS, Chicago, IL, USA), values were expressed as mean ± SEM, andPvalue <0.01 was considered significant. Effect of Sal B on expression of NF-kB p65 and IkBαwas studied and OD values of densitometry of western blots were taken. MPO activity was also studied. It was observed that treatment of Sal B significantly reduced the expression of both compounds in Sal B treated group as compared to control group after 28 days of treatment.

Download Full-text

Retracted: Study of Effect of Salvianolic Acid B on Motor Function Recovery in Rats with Spinal Cord Injury

BioMed Research International ◽

10.1155/2017/8234878 ◽

2017 ◽

Vol 2017 ◽

pp. 1-1

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Motor Function ◽

Salvianolic Acid B ◽

Salvianolic Acid ◽

Function Recovery ◽

Cord Injury

Download Full-text

Effect of fetal cerebellar tissue transplantation on the restoration of hind limb locomotor function in rats with spinal cord injury

Cell and Organ Transplantology ◽

10.22494/cot.v4i2.57 ◽

2016 ◽

Vol 4 (2) ◽

pp. 175-180

Author(s):

V. Medvediev ◽

Yu. Senchyk ◽

N. Draguntsova ◽

S. Dychko ◽

V. Tsymbaliuk

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Motor Function ◽

Hind Limb ◽

Tissue Transplantation ◽

Control Group ◽

Function Recovery ◽

Cord Injury ◽

Cerebellar Tissue ◽

Positive Effect

Fetal cerebellar tissue contains the largest number of neurogenic progenitors committed on the differentiation into glutamatergic neurons that can be used in the development of promising new treatment for spinal cord injuries.To evaluate the effect of fetal cerebellar tissue transplantation (FСTT) on the restoration of motor function after spinal cord injury in experiment.Materials and methods. Animals: inbred albino Wistar rats (5.5 months males, weighting 300 grams); main experimental groups: 1 – spinal cord injury + transplantation of a fragment of fetal (E18) rat cerebellum (n = 15), 2 – spinal cord injury only (n = 40). Model of an injury – left-side spinal cord hemisection at Т11; monitoring of the ipsilateral hind limb function (IHLF) – the Вasso-Вeattie-Вresnahan (BBB) scale.Results. FСTT normalizes the distribution of IHLF values, distorts the dynamics of the motor function recovery, transforming it from a progressive (in a control group) to the constant with variation within 3-3.6 points BBB during the experiment. FСTT causes early temporary positive effect on the functional state of the motor system, probably provided by mediator-dependent, neuroprotective, proangiogenic effect and remyelination. In our view, the gradual depletion of the FСTT positive effect due to resorption of the graft within the first 2 months is compensated by autoregenerative neoplastic process that is typical for the control group and by autoimmune utilization of myelin-associated inhibitors of axonal growth in the zone of injury that causes stability of the IHLF value during the observation period.Conclusion. Transplantation of fetal cerebellar tissue causes a short-term positive effect on the motor function recovery limited by the 1st month of the traumatic process. Evaluation of such type of neurotransplantation effectiveness requires taking into account the dynamics of the spasticity and chronic pain.

Download Full-text