scholarly journals Study of Cytotoxic Effects of Benzonitrile Pesticides

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Petra Lovecka ◽  
Marketa Thimova ◽  
Petra Grznarova ◽  
Jan Lipov ◽  
Zdenek Knejzlik ◽  
...  
Keyword(s):  
Cell Lines ◽  
Membrane Integrity ◽  
Human Cell Lines ◽  
Microbial Metabolites ◽  
Hep G2 ◽  
Cytotoxic Effects ◽  
Cell Membrane Integrity ◽  
Target Organs ◽  
Cytotoxic Compounds ◽  
Benzonitrile Herbicides

The benzonitrile herbicides bromoxynil, chloroxynil, dichlobenil, and ioxynil have been used actively worldwide to control weeds in agriculture since 1970s. Even though dichlobenil is prohibited in EU since 2008, studies addressing the fate of benzonitrile herbicides in the environment show that some metabolites of these herbicides are very persistent. We tested the cytotoxic effects of benzonitrile herbicides and their microbial metabolites using two human cell lines, Hep G2 and HEK293T, representing liver and kidneys as potential target organs in humans. The cell viability and proliferation were determined by MTT test and RTCA DP Analyzer system, respectively. The latter allows real-time monitoring of the effect of added substances. As the cytotoxic compounds could compromise cell membrane integrity, the lactate dehydrogenase test was performed as well. We observed high toxic effects of bromoxynil, chloroxynil, and ioxynil on both tested cell lines. In contrast, we determined only low inhibition of cell growth in presence of dichlobenil and microbial metabolites originating from the tested herbicides.

204 Cytotoxic effects of malachite green in two human cell lines

2003 ◽  
Vol 144 ◽  
pp. s58 ◽  
Author(s):  
I. De Angelis ◽  
A. Giuliano Albo ◽  
C. Nebbia ◽  
A. Stammati ◽  
F. Zampaglioni ◽  
...  
Keyword(s):  
Cell Lines ◽  
Malachite Green ◽  
Human Cell ◽  
Human Cell Lines ◽  
Cytotoxic Effects

Interferon potentiates cytotoxic effects of 5-fluorouracil on cell proliferation of established human cell lines originating from neoplastic tissues☆

1983 ◽  
Vol 17 (3) ◽  
pp. 239-247 ◽  
Author(s):  
Toshihiro Miyoshi ◽  
Shigeo Ogawa ◽  
Toshinori Kanamori ◽  
Masahiro Nobuhara ◽  
Masayoshi Namba
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Cytotoxic Effects ◽  
Neoplastic Tissues

scholarly journals Oleuropein-Rich Olive Leaf Extracts May Ameliorate Consequences of Glucose-Induced Oxidative Stress in Hep G2 Cells

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Mirjana Amidžić ◽  
Patricia Marić ◽  
Barbara Fumić ◽  
Roberta Petlevski ◽  
Kristina Bljajić ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Membrane Integrity ◽  
Radical Scavenger ◽  
Olive Leaf ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Leaf Extracts ◽  
Cell Membrane Integrity ◽  
Phenolic Constituents ◽  
G2 Cells

Olive leaf is reputed to have many beneficial health-related properties, often attributed to the iridoid glycoside oleuropein. In this work, the composition and antioxidant activity of oleuropein-rich olive leaf extracts prepared by ultrasonication were investigated. In addition, the influence of the extracts on Hep G2 cells subjected to glucose-induced oxidative stress was examined. The prepared extracts acted as antioxidants rich in oleuropein and other polyphenols. Among them, the extract prepared at 20°C using 100% ethanol was the most effective radical scavenger, antioxidant and metal chelator. It also contained the largest amount of oleuropein and other phenolic constituents. The extract prepared at 20°C using 70% ethanol, on the other hand, showed the best yield among the investigated extracts. Furthermore, it was able to preserve the cell-membrane integrity of Hep G2 cells subjected to glucose-induced oxidative stress, as well as to maintain intracellular concentration of glutathione and the activity of glutathione S-transferase on the levels comparable to those observed in non-stressed cells. The presented results demonstrate that oleuropein-rich olive leaf extracts can effectively ameliorate the consequences of glucose-induced oxidative stress in liver cells.

scholarly journals Effects of silver nanoparticles of different sizes on cytotoxicity and oxygen metabolism disorders in both reproductive and respiratory system cells

2016 ◽  
Vol 42 (4) ◽  
pp. 32-47 ◽  
Author(s):  
Lidia Zapór
Keyword(s):  
Silver Nanoparticles ◽  
Oxidative Dna Damage ◽  
Antimicrobial Agents ◽  
Membrane Integrity ◽  
Oxygen Metabolism ◽  
Protective Measures ◽  
Cytotoxic Effects ◽  
Cell Membrane Integrity ◽  
Antioxidative Status ◽  
Low Concentrations

Abstract Silver nanoparticles (AgNPs) are widely used in numerous industries and areas of daily life, mainly as antimicrobial agents. The particles size is very important, but still not suffi ciently recognized parameter infl uencing the toxicity of nanosilver. The aim of this study was to investigate the cytotoxic effects of AgNPs with different particle size (~ 10, 40 and 100 nm). The study was conducted on both reproductive and pulmonary cells (CHO-9, 15P-1 and RAW264.7). We tested the effects of AgNPs on cell viability, cell membrane integrity, mitochondrial metabolic activity, lipid peroxidation, total oxidative and antioxidative status of cells and oxidative DNA damage. All kinds of AgNPs showed strong cytotoxic activity at low concentrations (2÷13 μg/ml), and caused an overproduction of reactive oxygen species (ROS) at concentrations lower than cytotoxic ones. The ROS being formed in the cells induced oxidative damage of DNA in alkaline comet assay. The most toxic was AgNPs<10 nm. The results indicate that the silver nanoparticles, especially less than 10 nm, may be harmful to the organisms. Therefore, risk should be considered when using nanosilver preparations and provide appropriate protective measures when they are applied.

scholarly journals Synthesis and Pharmacological In Vitro Investigations of Novel Shikonin Derivatives with a Special Focus on Cyclopropane Bearing Derivatives

2021 ◽  
Vol 22 (5) ◽  
pp. 2774
Author(s):  
Nadine Kretschmer ◽  
Antje Hufner ◽  
Christin Durchschein ◽  
Katrin Popodi ◽  
Beate Rinner ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Membrane Integrity ◽  
Special Focus ◽  
Active Constituent ◽  
Cell Membrane Integrity ◽  
Shikonin Derivatives ◽  
Melanoma Cell Lines

Melanoma is the deadliest form of skin cancer and accounts for about three quarters of all skin cancer deaths. Especially at an advanced stage, its treatment is challenging, and survival rates are very low. In previous studies, we showed that the constituents of the roots of Onosma paniculata as well as a synthetic derivative of the most active constituent showed promising results in metastatic melanoma cell lines. In the current study, we address the question whether we can generate further derivatives with optimized activity by synthesis. Therefore, we prepared 31, mainly novel shikonin derivatives and screened them in different melanoma cell lines (WM9, WM164, and MUG-Mel2 cells) using the XTT viability assay. We identified (R)-1-(1,4-dihydro-5,8-dihydroxy-1,4-dioxonaphthalen-2-yl)-4-methylpent-3-enyl 2-cyclopropyl-2-oxoacetate as a novel derivative with even higher activity. Furthermore, pharmacological investigations including the ApoToxGloTM Triplex assay, LDH assay, and cell cycle measurements revealed that this compound induced apoptosis and reduced cells in the G1 phase accompanied by an increase of cells in the G2/M phase. Moreover, it showed hardly any effects on the cell membrane integrity. However, it also exhibited cytotoxicity against non-tumorigenic cells. Nevertheless, in summary, we could show that shikonin derivatives might be promising drug leads in the treatment of melanoma.

scholarly journals Studies on Cytotoxic Effects of Ochratoxin A Extracts on Human Cell Lines (Hfl-1 And Hep-2)

2016 ◽  
Vol 5 (7) ◽  
pp. 61-70 ◽  
Author(s):  
Jamal I. Elzwai ◽  
Mohamed A. Ahmed ◽  
Kofi A. Aidoo ◽  
Allan A. Candlish ◽  
Alyaa M. Homoud
Keyword(s):  
Ochratoxin A ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Cytotoxic Effects

Triflumuron induces cytotoxic effects on hepatic and renal human cell lines

2020 ◽  
Vol 34 (8) ◽  
Author(s):  
Rim Timoumi ◽  
Ines Amara ◽  
Intidhar Ben Salem ◽  
Salwa Abid‐Essefi
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Cytotoxic Effects

Quinoline-azetidinone hybrids: Synthesis and in vitro antiproliferation activity against Hep G2 and Hep 3B human cell lines

2017 ◽  
Vol 27 (7) ◽  
pp. 1566-1571 ◽  
Author(s):  
S.G. Alegaon ◽  
P. Parchure ◽  
L.D. Araujo ◽  
P.S. Salve ◽  
K.R. Alagawadi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Hep G2

The role of plant metabolism in the mutagenic and cytotoxic effects of four organophosphorus insecticides in Salmonella typhimurium and in human cell lines

Chemosphere ◽  
2013 ◽  
Vol 92 (9) ◽  
pp. 1117-1125 ◽  
Author(s):  
Josefina Cortés-Eslava ◽  
Sandra Gómez-Arroyo ◽  
Francisco Arenas-Huertero ◽  
Saúl Flores-Maya ◽  
Martha E. Díaz-Hernández ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Cell Lines ◽  
Human Cell ◽  
Plant Metabolism ◽  
Human Cell Lines ◽  
Organophosphorus Insecticides ◽  
Cytotoxic Effects
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