Interferon potentiates cytotoxic effects of 5-fluorouracil on cell proliferation of established human cell lines originating from neoplastic tissues☆

1983 ◽  
Vol 17 (3) ◽  
pp. 239-247 ◽  
Author(s):  
Toshihiro Miyoshi ◽  
Shigeo Ogawa ◽  
Toshinori Kanamori ◽  
Masahiro Nobuhara ◽  
Masayoshi Namba
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Cytotoxic Effects ◽  
Neoplastic Tissues

204 Cytotoxic effects of malachite green in two human cell lines

2003 ◽  
Vol 144 ◽  
pp. s58 ◽  
Author(s):  
I. De Angelis ◽  
A. Giuliano Albo ◽  
C. Nebbia ◽  
A. Stammati ◽  
F. Zampaglioni ◽  
...  
Keyword(s):  
Cell Lines ◽  
Malachite Green ◽  
Human Cell ◽  
Human Cell Lines ◽  
Cytotoxic Effects

scholarly journals HBO1 (KAT7) Does Not Have an Essential Role in Cell Proliferation, DNA Replication, or Histone 4 Acetylation in Human Cells

2019 ◽  
Vol 40 (4) ◽  
Author(s):  
Andrew J. Kueh ◽  
Samantha Eccles ◽  
Leonie Tang ◽  
Alexandra L. Garnham ◽  
Rose E. May ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Dna Replication ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cells ◽  
Human Cell Lines ◽  
Hela Cell Lines ◽  
Mouse Tissues ◽  
Sirna Knockdown

ABSTRACT HBO1 (MYST2/KAT7) is essential for histone 3 lysine 14 acetylation (H3K14ac) but is dispensable for H4 acetylation and DNA replication in mouse tissues. In contrast, previous studies using small interfering RNA (siRNA) knockdown in human cell lines have suggested that HBO1 is essential for DNA replication. To determine if HBO1 has distinctly different roles in immortalized human cell lines and normal mouse cells, we performed siRNA knockdown of HBO1. In addition, we used CRISPR/Cas9 to generate 293T, MCF7, and HeLa cell lines lacking HBO1. Using both techniques, we show that HBO1 is essential for all H3K14ac in human cells and is unlikely to have a direct effect on H4 acetylation and only has minor effects on cell proliferation. Surprisingly, the loss of HBO1 and H3K14ac in HeLa cells led to the secondary loss of almost all H4 acetylation after 4 weeks. Thus, HBO1 is dispensable for DNA replication and cell proliferation in immortalized human cells. However, while cell proliferation proceeded without HBO1 and H3K14ac, HBO1 gene deletion led to profound changes in cell adhesion, particularly in 293T cells. Consistent with this phenotype, the loss of HBO1 in both 293T and HeLa principally affected genes mediating cell adhesion, with comparatively minor effects on other cellular processes.

scholarly journals Lycopene has limited effect on cell proliferation in only two of seven human cell lines (both cancerous and noncancerous) in an in vitro system with doses across the physiological range

2008 ◽  
Vol 22 (5) ◽  
pp. 1297-1300 ◽  
Author(s):  
Lynn C. Burgess ◽  
Erin Rice ◽  
Tracy Fischer ◽  
Josh R. Seekins ◽  
Tyler P. Burgess ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Limited Effect ◽  
In Vitro System ◽  
Physiological Range

scholarly journals Studies on Cytotoxic Effects of Ochratoxin A Extracts on Human Cell Lines (Hfl-1 And Hep-2)

2016 ◽  
Vol 5 (7) ◽  
pp. 61-70 ◽  
Author(s):  
Jamal I. Elzwai ◽  
Mohamed A. Ahmed ◽  
Kofi A. Aidoo ◽  
Allan A. Candlish ◽  
Alyaa M. Homoud
Keyword(s):  
Ochratoxin A ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Cytotoxic Effects

Triflumuron induces cytotoxic effects on hepatic and renal human cell lines

2020 ◽  
Vol 34 (8) ◽  
Author(s):  
Rim Timoumi ◽  
Ines Amara ◽  
Intidhar Ben Salem ◽  
Salwa Abid‐Essefi
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Cytotoxic Effects

The role of plant metabolism in the mutagenic and cytotoxic effects of four organophosphorus insecticides in Salmonella typhimurium and in human cell lines

Chemosphere ◽  
2013 ◽  
Vol 92 (9) ◽  
pp. 1117-1125 ◽  
Author(s):  
Josefina Cortés-Eslava ◽  
Sandra Gómez-Arroyo ◽  
Francisco Arenas-Huertero ◽  
Saúl Flores-Maya ◽  
Martha E. Díaz-Hernández ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Cell Lines ◽  
Human Cell ◽  
Plant Metabolism ◽  
Human Cell Lines ◽  
Organophosphorus Insecticides ◽  
Cytotoxic Effects

scholarly journals An RNAi screen in human cell lines reveals conserved DNA damage repair pathways that mitigate formaldehyde sensitivity

2018 ◽  
Author(s):  
Eleonora Juarez ◽  
Nyasha Chambwe ◽  
Weiliang Tang ◽  
Asia D. Mitchell ◽  
Nichole Owen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Dna Damage ◽  
Ionizing Radiation ◽  
Cell Line ◽  
Cell Lines ◽  
Human Cell ◽  
Cancer Cell Line ◽  
Human Cell Lines ◽  
Genetic Determinants ◽  
Formaldehyde Toxicity

ABSTRACTFormaldehyde is a ubiquitous DNA damaging agent, with human exposures occuring from both exogenous and endogenous sources. Formaldehyde can also form DNA-protein crosslinks and is representative of other such DNA damaging agents including ionizing radiation, metals, aldehydes, chemotherapeutics, and cigarette smoke. In order to identify genetic determinants of cell proliferation in response to continuous formaldehyde exposure, we quantified cell proliferation after siRNA-depletion of a comprehensive array of over 300 genes representing all of the major DNA damage response pathways. Three unrelated human cell lines (SW480, U-2 OS and GM00639) were used to identify common or cell line-specific mechanisms. Four cellular pathways were determined to mitigate formaldehyde toxicity in all three cell lines: homologous recombination, double-strand break repair, ionizing radiation response, and DNA replication. Differences between cell lines were further investigated by using exome sequencing and Cancer Cell Line Encyclopedia genomic data. Our results reveal major genetic determinants of formaldehyde toxicity in human cells and provide evidence for the conservation of these formaldehyde responses between human and budding yeast.

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