scholarly journals Genetic Diversity of Selected Mangifera Species Revealed by Inter Simple Sequence Repeats Markers

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Zulhairil Ariffin ◽  
Muhammad Shafie Md Sah ◽  
Salma Idris ◽  
Nuradni Hashim
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Genetic Relatedness ◽  
Mangifera Indica ◽  
Issr Markers ◽  
Shannon Index ◽  
Inter Simple Sequence Repeats ◽  
High Degree ◽  
Simple Sequence ◽  
Dice Index

ISSR markers were employed to reveal genetic diversity and genetic relatedness among 28 Mangifera accessions collected from Yan (Kedah), Bukit Gantang (Perak), Sibuti (Sarawak), and Papar (Sabah). A total of 198 markers were generated using nine anchored primers and one nonanchored primer. Genetic variation among the 28 accessions of Mangifera species including wild relatives, landraces, and clonal varieties is high, with an average degree of polymorphism of 98% and mean Shannon index, H0=7.50. Analysis on 18 Mangifera indica accessions also showed high degree of polymorphism of 99% and mean Shannon index, H0=5.74. Dice index of genetic similarity ranged from 0.0938 to 0.8046 among the Mangifera species. The dendrogram showed that the Mangifera species were grouped into three main divergent clusters. Cluster 1 comprised 14 accessions from Kedah and Perak. Cluster II and cluster III comprised 14 accessions from Sarawak and Sabah. Meanwhile, the Dice index of genetic similarity for 18 accessions of Mangifera indica ranged from 0.2588 to 0.7742. The dendrogram also showed the 18 accessions of Mangifera indica were grouped into three main clusters. Cluster I comprised 10 landraces of Mangifera indica from Kedah. Cluster II comprised 7 landraces of Mangifera indica followed by Chokanan to form Cluster III.

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

2018 ◽  
Author(s):  
A. Chinapolaiah ◽  
K. Hima Bindu ◽  
G.N. Khadke ◽  
G.N. Manjesh ◽  
N. Hariprasad Rao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Issr Markers ◽  
Mucuna Pruriens ◽  
Diversity Analysis ◽  
Velvet Bean ◽  
Genetic Diversity Analysis ◽  
Inter Simple Sequence Repeats ◽  
Diversity Assessment ◽  
Simple Sequence

Velvet bean is an important medicinal legume, its seeds are prominent source of L-Dopa. The present investigation on genetic diversity assessment of 58 germplasm of velvet bean by using 11 ISSR markers. Out of 63 amplified products 59 were showed polymorphism and 4 were monomorphic with an average of 5.7 bands amplified per primer. According to band statistics and efficiency parameters showed the primers UBC 827, UBC 834 and UBC 836 were more efficient. The highest genetic similarity values (0.90) were observed between IIHR MP 102 and IIHR MP 74-3. In dendrogram germplasm grouped into two major clusters at 63 per cent similarity. Among the germplasm, IIHR Selection 4, IIHR Selection 10, IIHR MP 9, IC 33243 and IIHR MP 7 were found to be distinctly divergent, can be used in the further breeding programme.

Comparative assessment of genetic diversity among twenty varieties of Brassica juncea [L.] Czern & Coss using RAPD and ISSR markers

2012 ◽  
Vol 2 (4) ◽  
pp. 166-176
Author(s):  
Javed Ahmad ◽  
Salim Khan ◽  
Gohar Taj Khan
Keyword(s):  
Genetic Diversity ◽  
Brassica Juncea ◽  
Genetic Similarity ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Issr Markers ◽  
Rapd Profile ◽  
Inter Simple Sequence Repeats ◽  
Rapd And Issr ◽  
Simple Sequence

PCR based molecular markers such as RAPD (random amplified polymorphic DNA) and ISSR (inter simple sequence repeats) were employed for the evaluation of genetic diversity among twenty varieties of Brassica juncea. Mean polymorphism information content (PIC) value was greater for RAPD (0.4195) as compared to ISSR (0.2602). In RAPD analysis, 98.9% loci were polymorphic whereas in ISSR, 94.8 % were polymorphic. The number of loci in RAPD profile ranged from 7 to 10 with an average of 9.3 per primer whereas in ISSR, these were from 3 to 12 with an average of 6.8 loci per primer. RAPD based genetic similarity ranged from 0.224 to 0.842 whereas ISSR derived genetic similarity 0.467 to 0.880. The mental test between two Jaccard’s similarity matrices gave r = 0.89, showing good fit correlation in between ISSR‐ and RAPD‐based similarities. The results obtained from the consensus tree constructed from RAPD+ISSR marker more likely support the distribution of the twenty genotypes of B. juncea based on ISSR analysis. The twenty varieties were clustered into three main clusters 1, 2, and 3 respectively. In combined dendrogram study, each cluster has 13, 3, and 4 varieties.

Inter Simple Sequence Repeat (ISSR) markers to assess genetic diversity and relatedness within strawberry genotypes

2008 ◽  
Vol 88 (2) ◽  
pp. 313-322 ◽  
Author(s):  
S. C. Debnath ◽  
S. Khanizadeh ◽  
A. R. Jamieson ◽  
C. Kempler
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Genetic Similarity ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Group Method ◽  
Sequence Repeat ◽  
Breeding Lines ◽  
Pair Group ◽  
Simple Sequence

The goal of this study was to determine the level of genetic diversity and relatedness among 16 strawberry (Fragaria H ananassa Duch.) cultivars and 11 breeding lines developed in Canada, using Inter Simple Sequence Repeat (ISSR) markers. Seventeen primers generated 225 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic similarity among the genotypes ranging from 63 to 77% that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution for the place of breeding program explained only 1.4% of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR markers detected a sufficient degree of polymorphism to differentiate among strawberry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in current strawberry breeding programs. Key words: Fragaria × ananassa, DNA fingerprinting, multivariate analysis, breeding, genetic similarity

Genetic relatedness among cultivated and wild mulberry (Moraceae: Morus) as revealed by inter-simple sequence repeat analysis in China

2006 ◽  
Vol 86 (1) ◽  
pp. 251-257 ◽  
Author(s):  
Zhao Weiguo ◽  
Zhou Zhihua ◽  
Miao Xuexia ◽  
Wang Sibao ◽  
Zhang Lin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Genetic Relatedness ◽  
Genetic Relationships ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Group Method ◽  
Sequence Repeat ◽  
Pair Group ◽  
Simple Sequence

The genetic diversity of 27 mulberry (Morus spp.) genotypes mainly from China was investigated using inter-simple sequence repeat (ISSR) markers to assist in addressing breeding objectives and conserving existing genetic resources. Of the 22 primers screened, 15 produced highly reproducible ISSR bands. Using these 15 primers, 138 discernible DNA fragments were generated with 126 (91.3%) being polymorphic, indicating considerable genetic variation among the mulberry genotypes studied. Genetic similarity ranged from 0.6014 between Yu 2 and Yu 711 to 0.9493 between Cuizhisang and Dejiang 10. The phenetic dendrogram based on ISSR data generated by the unweighed pair group method with arithmetical averages (UPGMA) method grouped the 27 accessions into two major clusters: cluster I, cultivated mulberry species (M. multicaulis Perr., M. alba Linn., M. atropurpurea oxb., M. bombycis Kiodz., M. australis Poir., M. rotundiloba Kiodz., M. alba var. pendula Dipp., M. alba var. macrophylla Loud., and M. alba var. venose Delile.); and cluster II, wild mulberry species (M. cathayana Hemsl., M. laevigata Wall., M. wittiorum Hand-Mazz., M. nigra Linn., and M. mongolica Schneid.). Our molecular analyses agree with the existing morphological classification of Morus and clarify the genetic relationships among mulberry species. Key words: Morus L., genetic diversity, inter-simple sequence repeat, relatedness

Phylogenetic relationships between Leymus and related diploid Triticeae species revealed by ISSR markers

Biologia ◽  
2014 ◽  
Vol 69 (8) ◽  
Author(s):  
Guo-Ye Guo ◽  
Rui-Wu Yang ◽  
Chun-Bang Ding ◽  
Li Zhang ◽  
Yong-Hong Zhou ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Relationships ◽  
Diploid Species ◽  
Issr Markers ◽  
Similarity Coefficients ◽  
Triticeae Species ◽  
Inter Simple Sequence Repeats ◽  
Great Genetic Diversity ◽  
Simple Sequence ◽  
Issr Primers

AbstractTo investigate the genetic diversity and phylogenetic relationships between polyploid Leymus and related diploid species of the Triticeae tribe, inter-simple sequence repeats (ISSR) markers was used to analyze 41 Leymus accessions representing 22 species and 2 subspecies, together with Pseudoroegneria stipifolia (St), Psathyrostachys fragilis (Ns), Australopyrum retrofractum (W), Hordeum bogdanii, H. chilense (H) and Lophopyrum elongatum (Ee). A total of 376 clear and reproducible DNA fragments were amplified by 29 ISSR primers, among which 368 (97.87%) fragments were found to be polymorphic. 8–18 polymorphic bands were amplified by each polymorphic primer, with an average of 12.69 bands. The data of 376 ISSR bands were used to generate Nei’s similarity coefficients and to construct a dendrogram by means of UPGMA. The similarity coefficients data suggested great genetic diversity in genus Leymus and related diploid Triticeae species, the genetic diversity among the different species more abundant than that of the different accessions. The dendrogram and principal coordinate analysis showed explicit interspecific relationships and demonstrated close phylogenetic relationships between Leymus species and Psathyrostachys.

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