In VivoMonitoring on Growth and Spread of Gray Leaf Spot Disease inCapsicum annuumLeaf Using Spectral Domain Optical Coherence Tomography

We have demonstrated the application of optical coherence tomography (OCT) in diagnosis of growth and spread of the gray leaf spot disease inCapsicum annuumleaf caused by the fungusStemphylium lycopersici. Using 2D cross-sectional and 3D volumetric images of OCT,in vivostudy of layer differences between fungus infected leaves and healthy leaves was observed with distinctive features. We observed that the internal layers of the disease-affected parts of the leaf seem to merge forming a single thick layer. The obtained OCT results verify the noninvasive diagnosis ability of fungal growth and spread inCapsicum annuumleaves and the applicability of this methodology for other plant diseases.