scholarly journals Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Chong Zhao ◽  
Takenori Sakaguchi ◽  
Kosuke Fujita ◽  
Hideyuki Ito ◽  
Norihisa Nishida ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Activity ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Food Ingredients ◽  
Underlying Mechanisms ◽  
Species Production ◽  
Superoxide Dismutase 2

Pomegranate-derived polyphenols are expected to prevent life-style related diseases. In this study, we evaluated the ability of 8 pomegranate-derived polyphenols, along with other polyphenols, to augment SIRT3, a mammalian SIR2 homolog localized in mitochondria. We established a system for screening foods/food ingredients that augment the SIRT3 promoter in Caco-2 cells and identified 3 SIRT3-augmenting pomegranate-derived polyphenols (eucalbanin B, pomegraniin A, and eucarpanin T1). Among them, pomegraniin A activated superoxide dismutase 2 (SOD2) through SIRT3-mediated deacetylation, thereby reducing intracellular reactive oxygen species. The other SIRT3-augmenting polyphenols tested also activated SOD2, suggesting antioxidant activity. Our findings clarify the underlying mechanisms involved in the antioxidant activity of pomegraniin A.

Cucurbita argyrosperma seed extract ameliorating oxidative stress in H9c2 cardiomyocytes through suppression of intracellular reactive oxygen species production

2019 ◽  
Vol 15 (61) ◽  
pp. 327
Author(s):  
RosaMartha Perez-Gutierrez ◽  
MariaFernanda Estrella-Mendoza ◽  
JavierFlores Estrada ◽  
RebecaLopez Marure
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Seed Extract ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
H9c2 Cardiomyocytes ◽  
Species Production

scholarly journals Intracellular Reactive Oxygen Species Production by Polymorphonuclear Leukocytes in Bovine Leukemia Virus-Infected Dairy Cows

2012 ◽  
Vol 74 (2) ◽  
pp. 221-225 ◽  
Author(s):  
Fernando Nogueira de SOUZA ◽  
Maiara Garcia BLAGITZ ◽  
Andréia Oliveira LATORRE ◽  
Eduardo Milton RAMOS SANCHEZ ◽  
Camila Freitas BATISTA ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Dairy Cows ◽  
Bovine Leukemia Virus ◽  
Polymorphonuclear Leukocytes ◽  
Reactive Oxygen Species Production ◽  
Leukemia Virus ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Species Production

scholarly journals BIX-01294 induces autophagy-associated cell death via EHMT2/G9a dysfunction and intracellular reactive oxygen species production

Autophagy ◽  
2013 ◽  
Vol 9 (12) ◽  
pp. 2126-2139 ◽  
Author(s):  
Yunha Kim ◽  
Yong-Sook Kim ◽  
Dong Eun Kim ◽  
Jee Suk Lee ◽  
Ji Hoon Song ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Species Production

scholarly journals The effects of berberine on reactive oxygen species production in human neutrophils and in cell-free assays

2017 ◽  
Vol 10 (2) ◽  
pp. 61-65 ◽  
Author(s):  
Rami B. Kassab ◽  
Ondrej Vasicek ◽  
Milan Ciz ◽  
Antonin Lojek ◽  
Tomas Perecko
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Activity ◽  
Whole Blood ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Immune Defense ◽  
Peroxyl Radicals ◽  
Human Neutrophils ◽  
Oxygen Species ◽  
Species Production

AbstractThe health benefits of berberine have been recognized for years. Even so, its effects on human neutrophils, the first line of immune defense, have not been reported. The purpose of this study was to investigate the effects of berberine on the human neutrophil oxidative burst. Reactive oxygen species production was analyzed by luminol-enhanced chemiluminescence. The analysis was performed in spontaneous and stimulated (phorbol myristate acetate (PMA) or opsonized zymosan particles (OZP)) whole blood and isolated neutrophils in the presence or absence of berberine. The effects of berberine on oxidant production in cell-free assays were evaluated using luminescence (H2O2-peroxidase-luminol) and fluorescence (Oxygen Radical Absorbance Capacity – ORAC) techniques. Berberine decreased the production of reactive oxygen species in human whole blood and isolated neutrophils stimulated with either PMA or OZP with a different efficiency (EC50was 69 μM and 197 μM for PMA and OZP, respectively). The effect was more pronounced in isolated neutrophils. Cell-free assays showed the antioxidant activity of berberine against peroxyl radicals and hydrogen peroxide. Based on our results, we suggest that the effects of berberine on reactive oxygen species production in human neutrophils are due to its antioxidant activity.

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