scholarly journals Elucidating the Potential of Native Rhizobial Isolates to Improve Biological Nitrogen Fixation and Growth of Common Bean and Soybean in Smallholder Farming Systems of Kenya

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Ernest Wandera Ouma ◽  
Anne Mercy Asango ◽  
John Maingi ◽  
Ezekiel Mugendi Njeru

Identification of effective indigenous rhizobia isolates would lead to development of efficient and affordable rhizobia inoculants. These can promote nitrogen fixation in smallholder farming systems of Kenya. To realize this purpose, two experiments were conducted under greenhouse conditions using two common bean cultivars; Mwezi moja (bush type) and Mwitemania (climbing type) along with soybean cultivar SB 8. In the first experiment, the common bean cultivars were treated with rhizobia inoculants including a consortium of native isolates, commercial isolate (CIAT 899), a mixture of native isolates and CIAT 899, and a control with no inoculation. After 30 days, the crop was assessed for nodulation, shoot and root dry weights, and morphological features. In the second experiment, soybean was inoculated with a consortium of native isolates, commercial inoculant (USDA 110), and a mixture of commercial and native isolates. Remarkably, the native isolates significantly (p<0.001) increased nodulation and shoot dry weight across the two common bean varieties compared to the commercial inoculant, CIAT 899. Mixing of the native rhizobia species and commercial inoculant did not show any further increase in nodulation and shoot performance in both crops. Further field studies will ascertain the effectiveness and efficiency of the tested indigenous isolates.

2020 ◽  
pp. 1-12
Author(s):  
Trung Thanh Nguyen ◽  
Mary Atieno ◽  
Laetitia Herrmann ◽  
Sutkhet Nakasathien ◽  
Ed Sarobol ◽  
...  

Abstract In the Northern mountainous region of Vietnam, cassava–cowpea intercropping system has been widely promoted with support from the local agricultural department. However, cowpea yield is often limited because of a low Biological Nitrogen Fixation (BNF) activity due to its low natural nodulation and lack of available effective Rhizobium products. The aim of this study was to identify the most effective native rhizobia isolate nodulating cowpea with the potential to increase BNF and yield of cowpea. A greenhouse experiment was initially conducted with five treatments: three native rhizobia isolates (CMBP037, CMBP054, and CMBP065); a control (no inoculation and no N application); and N+ (no inoculation, application of N as KNO3). Field inoculations were carried out and the treatments were as follows: a control (no inoculation); CMBP (037+054) – a mixture of strains from Mau Dong; CMBP065 strain from Cat Thinh. CMBP054 and CMBP065 had the highest nodulation in the greenhouse (46.4 and 60.7 nodules plant−1, respectively) and were rated as effective with symbiotic efficiency (SEF) of 54.56 and 55.73%, respectively. In the field, CMBP (037+054) recorded significantly higher nodulation (19.4 nodules plant−1) than the control (11.7 nodules plant−1). CMBP (037+054) also increased cowpea shoot dry weight, shoot N, and yield by 28.6, 4.9, and 10.5%, respectively, compared to the uninoculated control. This effect was slope dependent (statistically significant in moderate and steep slope, not with gentle slope). Besides, the high expansion rate of intercropping with cowpea showed the high adoption level of these agroecological practices by local farmers. This study reveals the potential of native rhizobia inoculation to enhance soil fertility and sustainable agriculture in the Northern mountainous region of Vietnam and proposes enhanced efforts to promote the availability and utilization of effective inoculants for cowpea.


2019 ◽  
Vol 56 (2) ◽  
pp. 183-195 ◽  
Author(s):  
Tarirai Muoni ◽  
Eric Koomson ◽  
Ingrid Öborn ◽  
Carsten Marohn ◽  
Christine A. Watson ◽  
...  

AbstractOn low-input smallholder farms of Kenyan upland landscapes, erosion of nutrient-rich topsoil strongly affects crop yields. Where maize (Zea mays) is intercropped on erosion-prone slopes, intercropping can potentially reduce soil erosion. The objective of this research was to quantify the contribution of crops and crop mixtures of different growth habits to erosion control and their influence on above-ground biomass and earthworm abundance as indicators of soil function in smallholder farming systems under a bimodal rainfall pattern in Western Kenya. The experiment involved five treatments, namely maize (Z. mays)/common bean (Phaseolus vulgaris) intercrop (maize intercrop), maize/common bean intercrop plus Calliandra (Calliandra calothyrsus) hedgerows and Calliandra mulch (Calliandra), sole Lablab (Lablab purpureus), sole Mucuna (Mucuna pruriens) and groundnut (Arachis hypogaea) intercropped with maize (during the short rains). The experiment was conducted over three consecutive cropping seasons and the cropping system had significant effects on soil loss, runoff, water infiltration, earthworm abundance and above-ground biomass and crop grain yield. The Calliandra treatment had the lowest runoff (11.6–17.2 mm ha−1) and soil erosion (31–446 kg ha−1 per season) in all the seasons, followed by the Mucuna treatment. Lablab was affected by disease and showed the highest soil erosion in the last two seasons. Infiltration was highest in Calliandra treatment, and earthworm abundance was higher under Mucuna and Calliandra treatments (229 and 165 earthworms per square metre, respectively) than under other crops. Our results suggest that including sole crops of herbaceous species such as Mucuna, or tree hedgerows with mixtures of maize and grain legumes has the potential to reduce runoff and soil erosion in smallholder farming. Additionally, these species provide a suitable habitat for earthworms which stabilise soil structure and macropores and thus potentially increase infiltration, further reducing soil erosion.


Dairy ◽  
2021 ◽  
Vol 2 (3) ◽  
pp. 422-424
Author(s):  
Paola Scano ◽  
Pierluigi Caboni

Small ruminants, such as sheep and goats, are mostly raised in smallholder farming systems widely distributed throughout the world [...]


AMBIO ◽  
2012 ◽  
Vol 42 (3) ◽  
pp. 344-356 ◽  
Author(s):  
Isaline Jadin ◽  
Veerle Vanacker ◽  
Huong Thi Thu Hoang

Author(s):  
Alinne da Silva ◽  
◽  
Vinícius I. Franzini ◽  
Cristiano D. Piccolla ◽  
Takashi Muraoka ◽  
...  

ABSTRACT The common bean has been considered to have low biological nitrogen fixation capacity; however, this process can be made more effective with molybdenum (Mo) supplementation. The objective of this study was to evaluate the influence of Mo rates on the growth and biological nitrogen fixation by two Brazilian common bean cultivars using the 15N isotope dilution technique. The experiment was performed in 2014 in a completely randomized design arranged in a 5 x 3 factorial scheme, corresponding to 5 rates of Mo (control, 40, 80, 120 and 240 g ha-1), the common bean cultivars Aporé, Ouro Negro and NORH-54 (a non-nodulating common bean cultivar), and three replicates. The application of Mo and the inoculation with rhizobia strains contributed to improving nitrogen fixation and grain weight. The cultivar Ouro Negro showed a higher number and weight of nodules and a higher amount of nitrogen derived from the atmosphere than the cultivar Aporé. The biological nitrogen fixation of Aporé was more dependent on the application of Mo. These results indicated that inoculation with Rhizobium strains and Mo supply effectively contributed to biological nitrogen fixation and improving grain production.


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