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Dairy ◽  
2022 ◽  
Vol 3 (1) ◽  
pp. 47-58
Author(s):  
Lina Zhang ◽  
Sjef Boeren ◽  
Jeroen Heck ◽  
Jacques Vervoort ◽  
Peng Zhou ◽  
...  

Milk contains all nutrients needed for development of calves. One important group of components responsible for this are the milk proteins. Variation due to feed or animal health, has been studied for the most abundant milk proteins. The aim of this study was to determine the variation between and within cows for their milk serum proteome. Sample Set 1 was collected from Holstein Friesian (HF) cows between November 2011 and March 2012 and prepared using filter aided sample preparation (FASP) followed by LC-MS/MS for protein identification and quantification. The results showed that the milk serum proteome was very constant in mid lactation (four cows at five time points, p > 0.05) between 3 and 6 months in lactation. Sample Set 2 was collected from HF cows in Dec 2012 and analyzed using FASP and dimethyl labeling followed by LC-MS/MS. Significant variation in the milk serum proteome (p < 0.05) between 17 individual cows was found in Sample Set 2. The most variable proteins were immune-related proteins, which may reflect the health status of the individual cow. On the other hand, proteins related to nutrient synthesis and transport were relatively constant, indicating the importance of milk in providing a stable supply of nutrients to the neonate. In conclusion, the milk serum proteome was stable over mid lactation, but differed significantly between individuals, especially in immune-related proteins.


Dairy ◽  
2022 ◽  
Vol 3 (1) ◽  
pp. 29-46
Author(s):  
Shehadeh Kaskous

Milking machine design and performance are directly related to the milkability of sheep and goats, with the aim of milking quickly, completely and gently. This leads to an increase in the milk yield with improved quality, and the maintenance of healthy udders. The aim of this study was to carry out laboratory tests to determine the optimal level of vacuum, pulsation rate and pulsation ratio of new milking machines in high and low milk lines for sheep and goats. This study was conducted at the Department of Research and Development, Siliconform, Germany. For this purpose, different levels of vacuum (32, 34, 36, 38 and 40 kPa), milk jet (2, 2.5, 3 and 4 mm), milk line (high line and low line) and pulsation ratio (50:50 and 60:40) were used. First minute water flow (1st WF/kg) was used as an indicator for assessing the best combination in the milking machine. In addition, the cyclic vacuum fluctuation was measured in the inner chamber of the teat cup during the 1st WF/kg with the aid of a Vacuscope device. Statistical analysis was conducted using the mixed procedure in SAS. Our results show that the vacuum level, the milk jet and the pulsation ratio had a significant influence (p < 0.05) on the 1st WF/kg in the two milking machines for goats and sheep. In conclusion, the ideal conditions for milking goats with air inlet teat cups in the milking machine are a vacuum level of 36–38 kPa (low line) and 38–40 kPa (high line), a pulsation rate of 90 cycles/min and a pulsation ratio of 60:40, while the ideal conditions in the sheep milking machines are a vacuum level of 35–36 kPa (low line) and 36–38 kPa (high line), a pulsation rate of 120 cycles/min and a pulsation ratio of 60:40 or 50:50.


Dairy ◽  
2021 ◽  
Vol 3 (1) ◽  
pp. 12-28
Author(s):  
Panagiotis Simitzis ◽  
Christos Tzanidakis ◽  
Ouranios Tzamaloukas ◽  
Evangelia Sossidou

Although the effects of human–dairy cattle interaction have been extensively examined, data concerning small ruminants are scarce. The present review article aims at highlighting the effects of management practices on the productivity, physiology and behaviour of dairy animals. In general, aversive handling is associated with a milk yield reduction and welfare impairment. Precision livestock farming systems have therefore been applied and have rapidly changed the management process with the introduction of technological and computer innovations that contribute to the minimization of animal disturbances, the promotion of good practices and the maintenance of cattle’s welfare status and milk production and farms’ sustainability and competitiveness at high levels. However, although dairy farmers acknowledge the advantages deriving from the application of precision livestock farming advancements, a reluctance concerning their regular application to small ruminants is observed, due to economic and cultural constraints and poor technological infrastructures. As a result, targeted intervention training programmes are also necessary in order to improve the efficacy and efficiency of handling, especially of small ruminants.


Dairy ◽  
2021 ◽  
Vol 3 (1) ◽  
pp. 1-11
Author(s):  
Asahi Ogi ◽  
Marco Campera ◽  
Sara Ienco ◽  
Francesca Bonelli ◽  
Chiara Mariti ◽  
...  

Welfare assessment of dairy calves is commonly conducted through the observation of their behavior and the analysis of physiological parameters. Despite the large number of studies on this topic, there is a lack of research on the possible correlation between the physiological parameters and behavior at basal level in dairy calves. For this reason, the present study aimed to investigate the possible correlations between serum cortisol, neutrophil-to-lymphocyte ratio (N/L) and locomotor play behavior in 21 Friesian dairy calves within their first month of life. Every week, an arena test after blood collection was performed. A significant positive correlation between serum cortisol levels and N/L (rrm = 0.381; 95% CI = 0.083, 0.617; p = 0.012) and a negative correlation between time spent running and N/L (rrm = −0.351; 95% CI = −0.575, −0.077; p = 0.012) were found. On the contrary, no significant correlation between time spent running and cortisol levels was detected. Cortisol levels and N/L were higher in the newborn and had a constant decrease until reaching a stable value after the 24th day of life. These findings suggest a better predictivity of N/L, compared to serum cortisol, on locomotor play behavior.


Dairy ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 716-728
Author(s):  
Senén De La Torre-Santos ◽  
Luis J. Royo ◽  
Adela Martínez-Fernández ◽  
Mario Menéndez-Miranda ◽  
Rocío Rosa-García ◽  
...  

Dairy systems based on grass and forages are widely spread throughout the European Atlantic Arc and they have an influence on milk quality. Likewise, legumes are a key element in the farms to improve cows’ diet and farm feed self-sufficiency. The aim of this study was to evaluate the effect of the legumes in the diet and the feeding system (pasture-based vs. confined) on milk production and composition. An assay was performed with 18 Friesian cows randomized into two management groups (grazing or confined). Three total mixed rations based on Italian ryegrass, faba bean or field pea silages were offered ad libitum for nine continuously housed cows or during two hours after each milking for another nine grazing cows. Regardless of type of silage, grazing cows had higher dry matter intake and milk production than confined cows. Likewise, grazing cows produced milk with a lower concentration of protein and urea than confined cows. The dairy cows fed total mixed rations based on both legume silages had a milk fat with a higher proportion of unsaturated fatty acids, especially with the inclusion of faba bean silage in the diet. The results demonstrate that the profile of fatty acids and antioxidants is related to the feeding system in dairy cows. Grazing directly influenced the composition of milk, decreasing the proportion of saturated fatty acids and increasing the content of unsaturated fatty acids, as CLA, and the antioxidants, as lutein and β-cryptoxanthin.


Dairy ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 695-715
Author(s):  
Margaret E. Byrne ◽  
James A. O’Mahony ◽  
Tom F. O’Callaghan

Breastmilk is the optimal source of nutrition for infants. However, in circumstances where breastfeeding is not possible or feasible, infant formula provides an essential alternative to fulfil the nutritional requirements of the developing infant. Traditionally, the manufacture of infant formula has involved utilisation of bovine milk as a base ingredient, formulated with other nutrients and bioactive ingredients to closely match the composition of human breastmilk. While it is the most widely available type of formula on the market, bovine-based infant formula is not suitable for all infants, and therefore alternatives such as those based on caprine milk, soy and rice protein are becoming increasingly available. This review provides a detailed examination of the composition of infant formula prepared from bovine milk, caprine milk, soy, and rice protein sources. Available literature on nutrient bio-accessibility and aspects of protein functionality relevant to infant formula is discussed.


Dairy ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 684-694
Author(s):  
Lenka Krpálková ◽  
Niall O’Mahony ◽  
Anderson Carvalho ◽  
Sean Campbell ◽  
Gerard Corkery ◽  
...  

Identification of the associations of cow feed efficiency with feeding behaviour and milk production is important for supporting recommendations of strategies that optimise milk yield. The objective of this study was to identify associations between measures of feed efficiency, feed intake, feeding rate, rumination time, feeding time, and milk production using data collected from 26 dairy cows during a 3 month period in 2018. Cows averaged (mean ± standard deviation) 2.2 ± 1.7 lactations, 128 ± 40 days in milk, 27.5 ± 5.5 kg/day milk, 1.95 ± 0.69 kg feed/1 kg milk—the measure used to express feed conversion ratio (FCR), 575 ± 72 min/day rumination time, and 264 ± 67 min/day feeding time during the observation period. The coefficient of variation for rumination time (min/d) was 12.5%. A mixed linear model was selected for analyses. The most feed inefficient cows with the highest FCR (≥2.6 kg feed/1 kg milk) showed the lowest milk yield (24.8 kg/day), highest feed intake (78.8 kg), highest feeding rate (0.26 kg/min) and BCS (3.35 point). However, the relative milk yield (milk yield per 100 kg of body weight) was the highest (4.01 kg/day) in the most efficient group with the lowest FCR (≤1.4 kg feed/1 kg milk). Our study showed that the most efficient cows with the lowest FCR (≤1.4 kg feed/1 kg milk) had the highest rumination time (597 min/day; p < 0.05), feeding time (298 min/day; p < 0.05), rumination/activity ratio (4.39; p < 0.05) and rumination/feeding ratio (2.04; p < 0.05). Less active cows (activity time 164 min/day; p < 0.05) were the most efficient cows with the lowest FCR (≤1.4 kg feed/1 kg milk). The behavioural differences observed in this study provide new insight into the association of feed behaviour and feed efficiency with milk performance. Incorporating feeding behaviour into the dry matter intake model can improve its accuracy in the future and benefit breeding programmes.


Dairy ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 671-683
Author(s):  
Henrike Jansen ◽  
Marleen Zschiesche ◽  
Dirk Albers ◽  
Wilhelm Wemheuer ◽  
A. Sharifi ◽  
...  

This study gives an overview of the performance and accuracy of devices used for the fast measurement of β-hydroxybutyrate (BHBA) in blood for the on-farm indication of subclinical ketosis. Data were collected on ten dairy farms. In each farm, blood samples were taken from ten cows on four test days (2, 4, 9 and 11), resulting in 400 samples. The reference method was the BHBA concentration in blood serum (BHBALAB). Four different devices that measure BHBA in whole blood were tested. The thresholds applied for identifying subclinical ketosis were ≥1.0, ≥1.2 and ≥1.4 mmol/L in blood serum. The BHBALAB was assigned in three classes: low—≤0.9 mmol/L; high—>0.9 mmol/L; and total—all values unclassified. Due to initial negative effects on the health and performance of cows with BHBA levels ≥0.9 mmol/L, this cut-off was chosen. The Passing–Bablok regression revealed different constant as well as absolute biases for each device in the aforementioned classes. The area under the receiver operating characteristics curve indicated highly accurate results, with 94–97% accuracy levels. As an overall conclusion, the performance of the devices was good and supports their use by farmers for the detection of subclinical ketotic cows in their herds.


Dairy ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 658-670
Author(s):  
Melissa Di Rocco ◽  
Johann Scollard ◽  
Riona Sayers ◽  
Ambrose Furey ◽  
Martin Danaher ◽  
...  

The aim of this study was to investigate the distribution of cefquinome in different dairy products during the processing of naturally contaminated milk or spiked milk. The analysis of cefquinome residues in milk, skimmed milk, buttermilk, whey, cream, butter, curd, and cheese samples was performed using a water:acetonitrile solvent extraction and C18 dispersive solid-phase extraction (d-SPE) clean-up, followed by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS) determination. The target concentration of cefquinome was achieved in the spiked milk (100 µg kg−1). During its processing, the antibiotic migrated primarily with the skimmed milk as opposed to cream (ratios of 3.6:1 and 2.8:1 for experiments A and B, respectively), and with the buttermilk during butter manufacture (ratios of 6.9:1 and 4.6:1), but was equal in the curd and whey during the manufacture of cheese. In the milk collected from treated animals, the measured concentration of cefquinome was considerably high (approx. 5000 µg kg−1). The results obtained from the dairy products were similar to those obtained in the spiked study (ratios of 8.2:1 and 3.1:1 for experiments A and B, respectively, during the separation of skimmed milk and cream; 6.0:1 and 5.0:1 for A and B, respectively, during the separation of buttermilk and butter). However, during cheesemaking, cefquinome migrated with the whey after cutting the curd, with ratios of 0.54:1 and 0.44:1 for experiments A and B, respectively. The difference in the migration of cefquinome between curd and whey in spiked and animal studies is probably due to the different concentration levels in the two different experiments. The results of this study showed that, in dairy products manufactured from milk containing cefquinome residues, the drug migrated primarily with the high-water-containing fractions.


Dairy ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 649-657
Author(s):  
Liu Yang ◽  
Yahui Gao ◽  
Clarissa Boschiero ◽  
Li Li ◽  
Hongping Zhang ◽  
...  

Meiotic de novo mutation (DNM) is one of the important phenomena contributing to gamete genome diversity. However, except for humans and a few model organisms, they are not well studied in livestock, including cattle. Moreover, bulk sperm samples have been routinely utilized in experiments, which include millions of single sperm cells and only report high-frequency variants. In this study, we isolated and sequenced 143 single sperms from two Holstein bulls and identified hundreds of candidate DNM events in ten sperms with deep sequencing coverage. We estimated DNM rates ranging from 1.08 × 10−8 to 3.78 × 10−8 per nucleotide per generation. We further validated 12 out of 14 selected DNM events using Sanger sequencing. To our knowledge, this is the first single sperm whole-genome sequencing effort in livestock, which provided useful information for future studies of point mutations and male fertility. Our preliminary results pointed out future research directions and highlighted the importance of uniform whole genome amplification, deep sequence coverage, and dedicated software pipelines for genetic variant detection using single-cell sequencing data.


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