Management of Traumatic Cataract with Posterior Capsular Rupture: A Case Report and In Vitro Model Study

Purpose. To investigate the optimal strategy for surgical management of traumatic cataract with posterior capsular rupture. Methods. We describe four cases of traumatic cataract with posterior capsular rupture and an in vitro model built to evaluate the optimal infusion pressure during surgery. Results. All patients underwent cataract surgery. By using an anterior chamber maintainer to elevate infusion pressure, we safely performed cataract extraction without phacoemulsification. At 3 days after surgery, visual acuity was greater than 20/25 in all patients, without any complications. Phacoemulsification would also be feasible under anterior chamber maintainer infusion in a similar case of traumatic cataract with posterior capsular rupture during intravitreal injection. In addition, an in vitro model that we established using pig’s eyes revealed that the anterior chamber remained stable when the height of infusion bottle was 50–90 mmHg, whereas shallowing of the anterior chamber occurred when the height of infusion bottle was reduced to 40 mmHg, and corneal edema occurred when the height of infusion bottle was raised to 100 mmHg. Conclusions. During management of traumatic cataract with posterior capsular rupture, using an anterior chamber maintainer to maintain optimal infusion pressure may reduce the risk of anterior hyaloid membrane breakup and vitreous loss.

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Influence of biofilm removal from the tooth-restoration interface on the progression of secondary caries lesions: a preliminary in vitro model study

Biofouling ◽

10.1080/08927014.2020.1870219 ◽

2021 ◽

pp. 1-12

Author(s):

Cácia Signori ◽

Tamires Timm Maske ◽

Vitor Henrique Digmayer Romero ◽

Maximiliano Sérgio Cenci

Keyword(s):

In Vitro Model ◽

Secondary Caries ◽

Model Study ◽

Biofilm Removal ◽

Vitro Model ◽

Caries Lesions

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Effect of fluoride-releasing restorative materials on bacteria-induced pH fall at the bacteria–material interface: An in vitro model study

Journal of Dentistry ◽

10.1016/j.jdent.2013.11.006 ◽

2014 ◽

Vol 42 (1) ◽

pp. 15-20 ◽

Cited By ~ 22

Author(s):

Gen Mayanagi ◽

Koei Igarashi ◽

Jumpei Washio ◽

Hitomi Domon-Tawaraya ◽

Nobuhiro Takahashi

Keyword(s):

In Vitro Model ◽

Material Interface ◽

Model Study ◽

Restorative Materials ◽

Vitro Model

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Potential of Raloxifene in reversing osteoarthritis-like alterations in rat chondrocytes: An in vitro model study

Journal of Biosciences ◽

10.1007/s12038-012-9282-7 ◽

2012 ◽

Vol 38 (1) ◽

pp. 135-147 ◽

Cited By ~ 14

Author(s):

Aysegul Kavas ◽

Seda Tuncay Cagatay ◽

Sreeparna Banerjee ◽

Dilek Keskin ◽

Aysen Tezcaner

Keyword(s):

In Vitro Model ◽

Model Study ◽

Vitro Model

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Studies in Cranial Suture Biology: In Vitro Cranial Suture Fusion

The Cleft Palate-Craniofacial Journal ◽

10.1597/1545-1569_1996_033_0150_sicsbv_2.3.co_2 ◽

1996 ◽

Vol 33 (2) ◽

pp. 150-156 ◽

Cited By ~ 22

Author(s):

James P. Bradley ◽

Jamie P. Levine ◽

Christopher Blewett ◽

Thomas Krummel ◽

Joseph G. Mccarthy ◽

...

Keyword(s):

Organ Culture ◽

In Vitro Model ◽

In Vitro Study ◽

Cranial Base ◽

Cranial Suture ◽

Model Study ◽

Suture Fusion ◽

Vitro Model

The biology underlying craniosynostosis remains unknown. Previous studies have shown that the underlying dura mater, not the suture itself, signals a suture to fuse. The purpose of this study was to develop an in vitro model for cranial-suture fusion that would still allow for suture-dura interaction, but without the influence of tensional forces transmitted from the cranial base. This was accomplished by demonstrating that the posterior frontal mouse cranial suture, known to be the only cranial suture that fuses in vivo, fuses when plated with its dura in an organ-culture system. In such an organ-culture system, the sutures are free from both the influence of dural forces transmitted from the cranial base and from hormonal influences only available in a perfused system. For the cranial-suture fusion in vitro model study, the sagittal sutures (controls that remain patent in vivo) and posterior frontal sutures (that fuse in vivo) with the underlying dura were excised from 24-day-old euthanized mice, cut into 5 × 4 × 2-mm specimens, and cultured in a chemically defined, serum-free media. One hundred sutures were harvested at the day of sacrifice, then every 2 days thereafter until 30 days in culture, stained with H & E, and analyzed. A subsequent cranial-suture without dura in vitro study was performed in a similar fashion to the first study, but only the calvariae with the posterior frontal or sagittal sutures (without the underlying dura) were cultured. Results from the cranial-suture fusion in vitro model study showed that all sagittal sutures placed in organ culture with the underlying dura remained patent. More importantly, the posterior frontal sutures with the underlying dura, which were plated-down as patent at 24 days of age, demonstrated fusion after various growth periods in organ culture. In vitro posterior frontal mouse-suture fusion occurred in an anterior-to-posterior direction but in a delayed fashion, 4 to 7 days later than in vivo posterior frontal mouse-suture fusion. In contrast, the subsequent cranial-suture without dura in vitro study showed patency of all sutures, including the posterior frontal suture. These data from in vitro experiments indicate that: (1) mouse calvariae, sutures, and the underlying dura survive and grow in organ-culture systems for 30 days; (2) the local dura, free from external influences transmitted from the cranial base and hormones from distant sites, influences the cells of its overlying suture to cause fusion; and (3) without dura influence, all in vitro cranial sutures remained patent. By first identifying the factors involved in dural-suture signaling and then regulating these factors and their receptors, the biologic basis of suture fusion and craniosynostosis may be unraveled and used in the future to manipulate pathologic (premature) suture fusion.

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Cytotoxic and genotoxic potential of Bulgarian Rosa alba L. essential oil – in vitro model study

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2017.1423245 ◽

2018 ◽

Vol 32 (2) ◽

pp. 513-519 ◽

Cited By ~ 4

Author(s):

Gabrielle Jovtchev ◽

Alexander Stankov ◽

Almira Georgieva ◽

Anna Dobreva ◽

Rumiana Bakalova ◽

...

Keyword(s):

Essential Oil ◽

In Vitro Model ◽

Genotoxic Potential ◽

Model Study ◽

Vitro Model

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High-throughput, Label-Free Quantitative Proteomic Studies of the Anticancer Effects of Electrical Pulses with Turmeric Silver Nanoparticles: an in vitro Model Study

Scientific Reports ◽

10.1038/s41598-020-64128-8 ◽

2020 ◽

Vol 10 (1) ◽

Cited By ~ 4

Author(s):

Lakshya Mittal ◽

Ignacio G. Camarillo ◽

Gowri Sree Varadarajan ◽

Hemalatha Srinivasan ◽

Uma K. Aryal ◽

...

Keyword(s):

Silver Nanoparticles ◽

High Throughput ◽

In Vitro Model ◽

Label Free ◽

Anticancer Effects ◽

Model Study ◽

Electrical Pulses ◽

Vitro Model

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The Effect of Angle and Flow Rate Upon Hemodynamics in Distal Vascular Graft Anastomoses: An In Vitro Model Study

Journal of Biomechanical Engineering ◽

10.1115/1.2895427 ◽

1991 ◽

Vol 113 (4) ◽

pp. 458-463 ◽

Cited By ~ 92

Author(s):

R. S. Keynton ◽

S. E. Rittgers ◽

M. C. S. Shu

Keyword(s):

Stagnation Point ◽

Flow Rate ◽

In Vitro Model ◽

Laser Doppler Anemometry ◽

Bypass Graft ◽

Outer Wall ◽

Model Study ◽

Vitro Model ◽

Junction Angle

A steady flow, in vitro model of distal arterial bypass graft junctions was used to examine the effects of junction angle and flow rate on the local velocity field. Three test sections were fabricated from Plexiglas™ tubing having anastomotic junction angles of either 30, 45, or 60 deg. Flow visualization revealed velocity profiles skewed toward the outer wall with a flow split around a clear stagnation point along the outer wall. Laser Doppler anemometry [LDA] measurements confirmed a distinct stagnation point at the outer wall and both reverse and forward shear were detected immediately upstream and downstream, respectively, of this site. Axial velocities and shear rates along the outer wall were higher than along the inner wall and occurred in the junction angle order: 45, 60, and 30 deg. This study clearly identified changes in wall shear which varied with the anastomotic angle and flow rate.

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