Endovenous Laser Ablation of Varicose Veins Preserves Biological Properties of Vascular Endothelium and Modulates Proinflammatory Agent Profile More Favorably Than Classic Vein Stripping

Here we compared effect of serum from varicose patients undergoing endovenous laser ablation (EVLA) and classic vein stripping (CVS) on biological properties of endothelial cells and on the local and systemic profiles of proinflammatory agents. Results showed that serum from EVLA patients improved proliferation and reduced senescence and oxidative stress in the endothelial cells, as compared with the serum from CVS patients. These effects were related to a suppressed activity of TGF-β1, the level of which in the serum from the EVLA patients was decreased. Medium generated by the cells subjected to EVLA serum contained decreased amounts of ICAM-1, VCAM-1, and E-selectin and increased amount of uPA, whereas the serum itself contained decreased concentrations of ICAM-1, E-selectin, and P-selectin and increased concentrations of uPA, PAI-1, and TFPI. Both EVLA and CVS resulted in diversified patients’ reaction with respect to a direction of postprocedure changes in proinflammatory factors’ serum level. Analysis of proportions showed that the groups differed remarkably in case of ICAM-1 and ET-1, the level of which declined in a higher fraction of patients treated endovenously. Our findings indicate that EVLA preserves better than CVS the functionality of vascular endothelium and modulates better both local and systemic profile of proinflammatory mediators.

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Serum from Varicose Patients Induces Senescence-Related Dysfunction of Vascular Endothelium Generating Local and Systemic Proinflammatory Conditions

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/2069290 ◽

2016 ◽

Vol 2016 ◽

pp. 1-14 ◽

Cited By ~ 8

Author(s):

Justyna Mikuła-Pietrasik ◽

Paweł Uruski ◽

Krzysztof Aniukiewicz ◽

Patrycja Sosińska ◽

Zbigniew Krasiński ◽

...

Keyword(s):

Endothelial Cells ◽

Vascular Endothelium ◽

Varicose Veins ◽

Neutralizing Antibody ◽

Biological Properties ◽

Oxygen Species ◽

Healthy Donors ◽

Pai 1

Although the role of endothelium in varicose vein development is indisputable, the effect of the pathology on biological properties of endothelial cells remains unclear. Here we examined if the presence of varicose veins affects senescence of endothelial cells (HUVECs) and, if so, what will be the local and systemic outcome of this effect. Experiments showed that HUVECs subjected to serum from varicose patients display improved proliferation, increased expression of senescence marker, SA-β-Gal, and increased generation of reactive oxygen species (ROS), as compared with serum from healthy donors. Both increased SA-β-Gal activity and ROS release were mediated by TGF-β1, the concentration of which in varicose serum was elevated and the activity of which in vitro was prevented using specific neutralizing antibody. Senescent HUVECs exposed to varicose serum generated increased amounts of ICAM-1, VCAM-1, P-selectin, uPA, PAI-1, and ET-1. Direct comparison of sera from varicose and healthy donors showed that pathological serum contained increased level of ICAM-1, VCAM-1, P-selectin, uPA, and ET-1. Calendar age of healthy subjects correlated positively with serum uPA and negatively with P-selectin. Age of varicose patients correlated positively with ICAM-1, VCAM-1, and ET-1. Collectively, our findings indicate that the presence of varicose veins causes a senescence-related dysfunction of vascular endothelium, which leads to the development of local and systemic proinflammatory environment.

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The Management of Varicose Veins

International Surgery ◽

10.9738/intsurg-d-14-00084.1 ◽

2015 ◽

Vol 100 (1) ◽

pp. 185-189 ◽

Cited By ~ 12

Author(s):

Fan Lin ◽

Shiyi Zhang ◽

Yan Sun ◽

Shiyan Ren ◽

Peng Liu

Keyword(s):

Radiofrequency Ablation ◽

Laser Ablation ◽

Varicose Veins ◽

Controlled Study ◽

High Ligation ◽

Foam Sclerotherapy ◽

Current Management ◽

Endovenous Laser Ablation ◽

Randomized Controlled ◽

Better Than

Abstract This study aimed to review the current management modalities for varicose veins. There are a variety of management modalities for varicose veins. The outcomes of the treatment of varicose veins are different. The papers on the management of varicose veins were reviewed and the postoperative complications and efficacy were compared. Foam sclerotherapy and radiofrequency ablation were associated with less pain and faster recovery than endovenous laser ablation and surgical stripping. Patients undergoing endovenous laser ablation and radiofrequency ablation are most likely to have a faster recovery time and earlier return to work in comparison with those undergoing conventional high ligation and stripping. A randomized controlled study in multiple centers is warranted to verify which approach is better than others for the treatment of varicose veins.

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Complications and Failure of Endovenous Laser Ablation and Radiofrequency Ablation Procedures in Patients With Lower Extremity Varicose Veins in a 5-Year Follow-Up

Vascular and Endovascular Surgery ◽

10.1177/1538574416671247 ◽

2016 ◽

Vol 50 (7) ◽

pp. 475-483 ◽

Cited By ~ 13

Author(s):

Witold Woźniak ◽

R. Krzysztof Mlosek ◽

Piotr Ciostek

Keyword(s):

Radiofrequency Ablation ◽

Laser Ablation ◽

Lower Extremity ◽

Varicose Veins ◽

Endovenous Laser Ablation

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Endovenous laser ablation of varicose veins with the 1470-nm diode laser

Journal of Vascular Surgery ◽

10.1016/j.jvs.2010.01.027 ◽

2010 ◽

Vol 51 (6) ◽

pp. 1474-1478 ◽

Cited By ~ 89

Author(s):

Thomas Schwarz ◽

Eva von Hodenberg ◽

Christian Furtwängler ◽

Aljoscha Rastan ◽

Thomas Zeller ◽

...

Keyword(s):

Laser Ablation ◽

Diode Laser ◽

Varicose Veins ◽

Endovenous Laser Ablation

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Randomized clinical trial of endovenous laser ablation compared with conventional surgery for great saphenous varicose veins

British Journal of Surgery ◽

10.1002/bjs.7394 ◽

2011 ◽

Vol 98 (4) ◽

pp. 501-510 ◽

Cited By ~ 99

Author(s):

D. Carradice ◽

A. I. Mekako ◽

F. A. K. Mazari ◽

N. Samuel ◽

J. Hatfield ◽

...

Keyword(s):

Clinical Trial ◽

Laser Ablation ◽

Randomized Clinical Trial ◽

Varicose Veins ◽

Conventional Surgery ◽

Endovenous Laser Ablation

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Heat conduction from the exceedingly hot fiber tip contributes to the endovenous laser ablation of varicose veins

Lasers in Medical Science ◽

10.1007/s10103-008-0639-y ◽

2009 ◽

Vol 24 (2) ◽

pp. 247-251 ◽

Cited By ~ 31

Author(s):

Renate R. van den Bos ◽

Michael A. Kockaert ◽

H. A. Martino Neumann ◽

Rolf H. Bremmer ◽

Tamar Nijsten ◽

...

Keyword(s):

Heat Conduction ◽

Laser Ablation ◽

Varicose Veins ◽

Endovenous Laser Ablation

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Successful lysis in a stroke following endovenous laser ablation and extensive miniphlebectomy of varicose veins

Phlebology The Journal of Venous Disease ◽

10.1177/0268355515610235 ◽

2015 ◽

Vol 31 (4) ◽

pp. 296-298 ◽

Cited By ~ 2

Author(s):

Luca Spinedi ◽

Daniel Staub ◽

Heiko Uthoff

Keyword(s):

Ischemic Stroke ◽

Laser Ablation ◽

Thermal Ablation ◽

Patent Foramen Ovale ◽

Great Saphenous Vein ◽

Varicose Veins ◽

Foramen Ovale ◽

Fatal Complication ◽

Endovenous Laser Ablation ◽

Endovenous Thermal Ablation

Stroke is a very rare but potential fatal complication of endovenous thermal treatment in patients with a right-to-left shunt. To our best knowledge, there are only two reports in the literature of stroke after endovenous thermal ablation of varicose veins, one after endovenous laser ablation and one after radiofrequency ablation and phlebectomy, both treated conservatively. This report describes a successful lysis in a patient with an ischemic stroke associated with bilateral endovenous heat-induced thrombosis class I after endovenous laser ablation of both great saphenous vein and extensive miniphlebectomy in a patient with an unknown patent foramen ovale.

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