scholarly journals Effect of Free Ammonia, Free Nitrous Acid, and Alkalinity on the Partial Nitrification of Pretreated Pig Slurry, Using an Alternating Oxic/Anoxic SBR

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Marisol Belmonte ◽  
Chia-Fang Hsieh ◽  
José Luis Campos ◽  
Lorna Guerrero ◽  
Ramón Méndez ◽  
...  
Keyword(s):  
Nitrous Acid ◽  
Batch Reactor ◽  
Inhibitory Effect ◽  
Free Ammonia ◽  
Partial Nitrification ◽  
Total Alkalinity ◽  
Pig Slurry ◽  
Solid Retention Time ◽  
Free Nitrous Acid ◽  
Nitrite Oxidizing Bacteria

The effect of free ammonia (NH3or FA), free nitrous acid (HNO2or FNA), and total alkalinity (TA) on the performance of a partial nitrification (PN) sequencing batch reactor (SBR) treating anaerobically pretreated pig slurry was studied. The SBR was operated under alternating oxic/anoxic (O/A) conditions and was fed during anoxic phases. This strategy allowed using organic matter to partially remove nitrite (NO2-) and nitrate (NO3-) generated during oxic phases. The desiredNH4+toNO2-ratio of 1.3 g N/g N was obtained when an Ammonium Loading Rate (ALR) of 0.09 gNH4+-N/L·d was applied. The system was operated at a solid retention time (SRT) of 15–20 d and dissolved oxygen (DO) levels higher than 3 mg O2/L during the whole operational period. PN mainly occurred caused by the inhibitory effect of FNA on nitrite oxidizing bacteria (NOB). Once HNO2concentration was negligible,NH4+was fully oxidized toNO3-in spite of the presence of FA. The use of biomass acclimated to ammonium as inoculum avoided a possible effect of FA on NOB activity.

Free ammonia and free nitrous acid inhibition on the anabolic and catabolic processes of Nitrosomonas and Nitrobacter

2007 ◽  
Vol 56 (7) ◽  
pp. 89-97 ◽  
Author(s):  
V.M. Vadivelu ◽  
J. Keller ◽  
Z. Yuan
Keyword(s):  
Nitrous Acid ◽  
Energy Production ◽  
Inhibitory Effect ◽  
Free Ammonia ◽  
Fish Analysis ◽  
Batch Reactors ◽  
Inhibitory Effects ◽  
Free Nitrous Acid ◽  
Production Capability ◽  
Batch Tests

The inhibitory effects of free ammonia (FA) and free nitrous acid (FNA) on the catabolic and anabolic processes of Nitrosomonas and Nitrobacter were investigated using a method that allows decoupling the growth and energy generation processes. Lab-scale sequencing batch reactors (SBRs) were operated for the enrichment of Nitrosomonas and Nitrobacter. Fluorescent In-Situ Hybridization (FISH) analysis showed that the reactors were 82% and 73% enriched with Nitrosomonas and Nitrobacter, respectively. Batch tests were carried out to measure the oxygen uptake rate (OUR) by the enriched cultures at various FA and FNA levels, in the presence (OURwithCO2) or absence (OURwithoutCO2) of inorganic carbon (CO2, HCO3− and CO32−). FA up to 16.0 mgNH3-N.L−1 was not found to have any inhibitory effect on either the catabolic or anabolic processes of the Nitrosomonas culture, but both these processes were inhibited by FNA. While an FNA level of 0.40–0.63 mgHNO2-N.L−1 inhibited the energy production capability of Nitrosomonas by 50%, the growth process of the culture was completely inhibited by FNA at a concentration of 0.40 mgHNO2-N.L−1. Both FA and FNA were found to have strong inhibition on the anabolic processes of Nitrobacter, but with limited inhibitory effects on the catabolism of this culture. The biosynthesis of Nitrobacter was totally inhibited at an FA level of 6.0 mgNH3-N.L−1 (or above) or an FNA level of 0.02 mgHNO2-N.L−1 (or above). At the same level of FA, the energy production capability of Nitrobacter was only inhibited by 12%, whereas an FNA level of up to 0.024 mgHNO2-N.L−1 did not show any inhibition on the energy production of Nitrobacter. Further, these inhibitory effects appears to be much stronger on Nitrobacter than on Nitrosomonas, supporting that FA and FNA inhibition may play a major role in the elimination of nitrite oxidizing bacteria in processes treating wastewater containing a high level of nitrogen.

Effect of influent nitrogen concentration on feasibility of short-cut nitrification during wastewater treatment in activated sludge systems

2015 ◽  
Vol 69 (7) ◽  
Author(s):  
Pavel Svehla ◽  
Josef Radechovsky ◽  
Helena Hrncirova ◽  
Lukas Pacek ◽  
Jan Bartacek
Keyword(s):  
Wastewater Treatment ◽  
Nitrous Acid ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Nitrogen Concentration ◽  
Inhibitory Effect ◽  
Microbial Culture ◽  
Dissolved Oxygen Concentration ◽  
Free Nitrous Acid ◽  
Activated Sludge Systems

AbstractThe inhibitory effect of free ammonia and free nitrous acid on nitrite-oxidising bacteria (NOB) was studied in a laboratory-scale sequencing batch reactor with a suspended microbial culture. The reactor was operated at 15°C, with a dissolved oxygen concentration in excess of 5 mg L

Inhibition effect of free ammonia and free nitrous acid on nitrite-oxidising bacteria during sludge liquor treatment: influence of feeding strategy

2014 ◽  
Vol 68 (7) ◽  
Author(s):  
Pavel Svehla ◽  
Jan Bartacek ◽  
Lukas Pacek ◽  
Helena Hrncirova ◽  
Josef Radechovsky ◽  
...  
Keyword(s):  
Nitrous Acid ◽  
Feeding Strategy ◽  
Batch Reactor ◽  
Ammonia Nitrogen ◽  
Free Ammonia ◽  
Reactor Operation ◽  
High Concentration ◽  
Free Nitrous Acid ◽  
Operation Regime ◽  
Inhibition Effect

AbstractThe importance of feeding strategy for the long-term selective inhibition of nitrite-oxidising bacteria (NOB) was demonstrated by comparison of laboratory-scale bioreactors: Completely Stirred Tank Reactor (CSTR) and Sequencing Batch Reactor (SBR). Moreover, the effect of the change of reactor operation regime from CSTR to SBR was demonstrated. Sludge liquor containing ammonia nitrogen in a range of 970–1500 mg L−1 was the influent of the reactors. The experiments were performed at (23 ± 2)°C, with high concentration of dissolved oxygen (up to 8 mg L−1) and with unlimited sludge retention time. In the SBR, permanent restriction of NOB activity was achieved for more than 700 days by the strong inhibition effect of fluctuating concentrations of free ammonia and free nitrous acid during the operational cycles of SBR. In contrast, nitrite-oxidising bacteria were able to gradually adapt to the conditions prevailing in CSTR and produce nitrate although the concentration of free ammonia and free nitrous acid significantly exceeded inhibition limits for NOB activity in this system. Transferring the reactor operation regime from CSTR to SBR resulted in immediate and permanent inhibition of NOB activity in the reactor.

Comprehensive assessment of free nitrous acid-based technology to establish partial nitrification

2018 ◽  
Vol 4 (12) ◽  
pp. 2113-2124
Author(s):  
Cancan Jiang ◽  
Shengjun Xu ◽  
Rui Wang ◽  
Sining Zhou ◽  
Shanghua Wu ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Nitrous Acid ◽  
Comprehensive Assessment ◽  
Partial Nitrification ◽  
Free Nitrous Acid ◽  
Promising Technology ◽  
Nitrite Oxidizing Bacteria

Treating activated sludge with free nitrous acid (FNA) to selectively eliminate nitrite-oxidizing bacteria (NOB) and retain ammonium-oxidizing bacteria (AOB) is increasingly regarded as a promising technology for achieving partial nitrification.

Biological Treatment of High Ammonium Content Urban Landfill Leachate Using an Anaerobic-Aerobic Process

2013 ◽  
Vol 781-784 ◽  
pp. 2095-2099
Author(s):  
Hong Wei Sun ◽  
Yong Jun You ◽  
Ying Guo
Keyword(s):  
Landfill Leachate ◽  
Inhibitory Effect ◽  
Nitrifying Bacteria ◽  
Anaerobic Sludge ◽  
Nitrite Accumulation ◽  
Fish Analysis ◽  
Free Nitrous Acid ◽  
Nitrite Oxidizing Bacteria ◽  
Anaerobic Sludge Blanket

Biological system consisting of an up-flow anaerobic sludge blanket (UASB) and anoxic-oxic (A/O) reactor was applied to treat high ammonium content urban landfill leachate. Inhibitory effect of free ammonia (FA) and free nitrous acid (FNA) on nitrifying bacteria activity was used to achieve nitrogen removal via nitrite pathway in the A/O. Results demonstrated that removed efficiencies of COD, total nitrogen (TN) and NH4+-N were 95.3%, 84.6 %and 99.2%, respectively. Stable nitrite pathway with above 90% nitrite accumulation was successfully achieved in the A/O reactor by synergetic inhibition of FA and FNA on the activity of nitrite oxidizing bacteria (NOB). Moreover, Fluorescence in situ hybridization (FISH) analysis showed that AOB was dominant microorganism.

Achieving nitrogen removal via nitrite pathway from urban landfill leachate using the synergetic inhibition of free ammonia and free nitrous acid on nitrifying bacteria activity

2013 ◽  
Vol 68 (9) ◽  
pp. 2035-2041 ◽  
Author(s):  
H. W. Sun ◽  
Y. Bai ◽  
Y. Z. Peng ◽  
H. G. Xie ◽  
X. N. Shi
Keyword(s):  
Landfill Leachate ◽  
Biological System ◽  
Nitrous Acid ◽  
Free Ammonia ◽  
Nitrifying Bacteria ◽  
Anaerobic Sludge ◽  
Nitrite Accumulation ◽  
Fish Analysis ◽  
Ammonia Oxidizing Bacteria ◽  
Free Nitrous Acid

In this study, a biological system consisting of an up-flow anaerobic sludge blanket (UASB) and anoxic–oxic (A/O) reactor was established for the advanced treatment of high ammonium urban landfill leachate. The inhibitory effect of free ammonia (FA) and free nitrous acid (FNA) on the nitrifying bacterial activity was used to achieve stable nitritation in the A/O reactor. The results demonstrated that the biological system achieved chemical oxygen demand (COD), total nitrogen (TN) and NH4+-N removal efficiencies of 95.3, 84.6 and 99.2%, respectively at a low carbon-to-nitrogen ratio of 3:1. Simultaneous denitritation and methanogenesis in the UASB could improve the removal of COD and TN. Nitritation with above 90% nitrite accumulation was successfully achieved in the A/O reactor by synergetic inhibition of FA and FNA on the activity of nitrite oxidizing bacteria (NOB). Fluorescence in situ hybridization (FISH) analysis showed that ammonia oxidizing bacteria (AOB) was dominant and was considered to be responsible for the satisfactory nitritation performance.

Nitrogen removal from pharmaceutical manufacturing wastewater with high concentration of ammonia and free ammonia via partial nitrification and denitrification

2004 ◽  
Vol 50 (6) ◽  
pp. 31-36 ◽  
Author(s):  
Y.Z. Peng ◽  
Y.Z. Li ◽  
C.Y. Peng ◽  
S.Y. Wang
Keyword(s):  
Nitrogen Removal ◽  
Accumulation Rate ◽  
Batch Reactor ◽  
Free Ammonia ◽  
Pharmaceutical Manufacturing ◽  
Partial Nitrification ◽  
High Concentration ◽  
Wastewater Stream ◽  
Nitrification And Denitrification ◽  
Study Laboratory

In this study, laboratory-scale experiments were conducted applying a Sequencing Batch Reactor (SBR) activated sludge process to a wastewater stream from a pharmaceutical factory. Nitrogen removal can be achieved via partial nitrification and denitrification and the efficiency was above 99% at 23°C ± 1. The experimental results indicated that the nitrite oxidizers were more sensitive than ammonia oxidizers to the free ammonia in the wastewater. The average accumulation rate of nitrite was much higher than that of nitrate. During nitrogen removal via the nitrite pathway, the end of nitrification and denitrification can be exactly decided by monitoring the variation of pH. Consequently, on-line control for nitrogen removal from the pharmaceutical manufacturing wastewater can be achieved and the cost of operation can be reduced.

Model-based study of nitrite accumulation with OUR control in two continuous nitrifying activated sludge configurations

2009 ◽  
Vol 60 (10) ◽  
pp. 2685-2693 ◽  
Author(s):  
Irene Jubany ◽  
Juan Antonio Baeza ◽  
Javier Lafuente ◽  
Julian Carrera
Keyword(s):  
Partial Nitrification ◽  
Nitrite Accumulation ◽  
Good Tool ◽  
Free Nitrous Acid ◽  
Stirred Tank Reactors ◽  
Inhibitory Compounds ◽  
Nitrite Oxidizing Bacteria ◽  
In Series ◽  
Continuous Stirred Tank ◽  
Different Characteristics

Achievement of partial nitrification relies on the different characteristics of the microorganisms involved in the two steps of nitrification (AOB, ammonium oxidizing bacteria and NOB, nitrite oxidizing bacteria). Several configurations and conditions have been used to achieve partial nitrification taking into account their different responses in front of some environmental conditions like pH, temperature (T), dissolved oxygen (DO) and the inhibitory compounds free ammonia (FA) and free nitrous acid (FNA). This work is a theoretical study about the utilization of a control strategy based on controlling OUR by manipulating the influent flow-rate together with additional changes in T, pH or DO for achieving stable partial nitrification. Two different configurations were simulated. Configuration A consisted of three continuous stirred-tank reactors in series plus a settler, while configuration B consisted of a single reactor with the same total volume and a settler. The developed control system was shown as a good tool to achieve partial nitrification with both configurations. Nevertheless, configuration A showed better results and partial nitrification was obtained even under theoretically unfavourable conditions as T = 15°C, pH = 8.3 and DO = 3 mg O2 L−1.

Partial Nitrification to Nitrite with Real-Time Aeration Duration Control in an SBR Treating Domestic Wastewater

2011 ◽  
Vol 356-360 ◽  
pp. 1046-1049 ◽  
Author(s):  
Yu Chen ◽  
Jun Li ◽  
C .W Wang ◽  
X.F Zhao ◽  
B.H Zhao
Keyword(s):  
Real Time ◽  
Batch Reactor ◽  
Domestic Wastewater ◽  
Partial Nitrification ◽  
Nitrite Accumulation ◽  
Ammonia Oxidizing Bacteria ◽  
Term Operation ◽  
Nitrite Oxidizing Bacteria ◽  
Low Strength

Sustainable partial nitrification to nitrite has been proven difficult in treating low strength nitrogenous wastewater. Real-time aeration duration control was used to achieve efficient partial nitrification to nitrite in a sequencing batch reactor (SBR) to treat low strength domestic wastewater. Above 95% nitrite accumulation ratio was maintained for long-term operation at normal condition. Partial nitrification established by controlling aeration duration showed good performance and robustness even though some time encountering long-term extended aeration. Process control enhanced the successful accumulation of ammonia oxidizing bacteria (AOB) and washout of nitrite oxidizing bacteria (NOB).

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