Trueperella pyogenesandBrucella abortusCoinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of Mastitis and Abortion

Trueperella pyogeneswas isolated from a dog and a cat with a mixed infection withBrucella abortus. Both lived on a dairy cattle farm with a history of regular cases of abortion and mastitis. Identification of the bacteria was done by means of MALDI-TOF MS, loop-mediated isothermal amplification (LAMP) based oncpn60, partial 16S rRNA sequencing, and growth on Loeffler Serum Medium. Isolation ofTrueperella pyogeneson the dairy farm highlights its neglected role in reproduction failure and draws attention to its effects in the dairy industry in Egypt. Diagnosis and control of abortion in Egypt should includeTrueperella pyogenesas one of possible causes of abortion.

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Reproductive Disorders and Seroprevalence of Brucellosis in Yak

Nepal Agriculture Research Journal ◽

10.3126/narj.v8i0.11608 ◽

2014 ◽

Vol 8 ◽

pp. 130-132 ◽

Cited By ~ 3

Author(s):

Sitaram Aryal ◽

Krishna P. Paudel

Keyword(s):

Adult Female ◽

Brucella Abortus ◽

Random Sampling ◽

Elisa Test ◽

Reproductive Disorders ◽

Serum Samples ◽

Retained Placenta ◽

History Of ◽

And Control

A study on reproductive disorders among yaks in Mustang and Solukhumbu districts and seroprevalence of brucellosis among yaks in Mustang and Myagdi districts was conducted. Eleven farmers having 383 female and 72 male yaks in Mustang and 129 female and 27 male yaks in Solukhumbu districts were taken by purposive random sampling for the study on reproductive disorders. Likewise Sixty-seven serum samples were also collected from adult female yaks having history of abortion and retained placenta, and from male yaks used for breeding purpose from Mustang and Myagdi districts. An average of 12.63% of female yaks aborted, 7.26% had had retention of placenta and 6.6% repeat breeders in Mustang district. Similarly, 6.3% had abortion, 2.09% retained placenta and 6.5% repeat breeders in Solukhumbu district. However, all the 67 serum samples examined were found negative to the Brucella abortus antibody on ELISA test suggesting that the yak population was free from brucellosis in these areas. Thus abortion and retention of placenta in Yak in Mustang district may not be due to brucellosis. A detail study should be done to find the specific cause of abortion and repeat breeding to recommend preventive and control measures.Nepal Agric. Res. J. Vol. 8, 2007, pp. 130-132 DOI: http://dx.doi.org/10.3126/narj.v8i0.11608

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MALDI-TOF MS versus 16S rRNA sequencing: Minor discrepancy between tools in identification of Bacteroides isolates

Acta Microbiologica et Immunologica Hungarica ◽

10.1556/030.64.2017.025 ◽

2018 ◽

Vol 65 (2) ◽

pp. 173-181

Author(s):

Károly Péter Sárvári ◽

József Sóki ◽

Miklós Iván ◽

Cecilia Miszti ◽

Krisztina Latkóczy ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Sequencing ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Rrna Sequencing ◽

Tof Ms

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Comparision of two identification methods: MALDI-TOF-MS - 16s rRNA sequencing in enterococci isolated from raw milk cheeses (Sikma cheeses) and evaluating their antibiotic susceptibility and antimicrobial activity

Turkish Bulletin of Hygiene and Experimental Biology ◽

10.5505/turkhijyen.2020.92332 ◽

2020 ◽

Vol 77 (4) ◽

pp. 399-412

Author(s):

Furkan AYDIN ◽

Halil İbrahim KAHVE ◽

Mustafa ARDIÇ ◽

İbrahim ÇAKIR

Keyword(s):

Antimicrobial Activity ◽

16S Rrna ◽

Antibiotic Susceptibility ◽

Raw Milk ◽

16S Rrna Sequencing ◽

Identification Methods ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Rrna Sequencing ◽

Tof Ms

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Identification of Lysinibacillus fusiformis Isolated from Cosmetic Samples Using MALDI-TOF MS and 16S rRNA Sequencing Methods

Journal of AOAC International ◽

10.5740/jaoacint.18-0092 ◽

2018 ◽

Vol 101 (6) ◽

pp. 1757-1762 ◽

Cited By ~ 1

Author(s):

Irshad M Sulaiman ◽

Ying-Hsin Hsieh ◽

Emily Jacobs ◽

Nancy Miranda ◽

Steven Simpson ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Sequencing ◽

Gram Positive ◽

Maldi Tof Ms ◽

Lysinibacillus Fusiformis ◽

Maldi Tof ◽

Microbiological Methods ◽

Rrna Sequencing ◽

Level Identification ◽

Tof Ms

Abstract Background: Lysinibacillus fusiformis is a Gram-positive, rod-shaped bacterium that can cause tropical ulcers, severe sepsis, and respiratory illnesses in humans. Objective: In this study, we analyzed cosmetic samples for the presence of human pathogenic microorganisms. Methods: Five unopened jars of exfoliating cream were examined initially by microbiological methods. Afterward, matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) MS and 16S ribosomal RNA (rRNA) sequencing techniques were applied to characterize the recovered isolates. Results: Of the eight recovered Gram-positive bacterial subs, the VITEK® MS could provide genus-level identification to five subs and species-level identification to two subs (L. fusiformis with a 99.9% confidence value); one sub was unidentified. Subsequently, the deoxyriboneucleic acid sequencing of the 16S rRNA gene was done on an ABI 3500XL Genetic Analyzer for the confirmation of species identification. An analysis of sequencing data revealed a complete absence of genetic variation among the eight subs sequenced at this locus and confirmed the eight bacterial subs to be L. fusiformis, as their respective 16S rRNA sequences were identical to the available sequence in public domain (GenBank accession No. KU179364). Conclusions: Our results suggest that the VITEK MS and the 16S rRNA sequencing can be used for the identification of human pathogenic bacteria of public health importance. Highlights: We characterized eight isolates of Lysinibacillus spp. from cosmetics by MALDI-TOF MS and 16S rRNA sequence analyses.

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